Nicotinamide riboside |
| رقم الكتالوجGC44401 |
يتم تحويل النيكوتيناميد رايبوسايد، وهو شكل من أشكال فيتامين ب3 ومادة مسبقة لـ NAD+، إلى NAD+ المتاح حيوية عبر نزع فسفات من قِبَل إنزيم NRK (nicotinamide riboside kinase) و NMNAT، أو عبر عملية الإضافة التأرجحية لفسفات النُّكْلِئُوزِ وإعادة استخدام NAM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1341-23-7
Sample solution is provided at 25 µL, 10mM.
Nicotinamide riboside, a form of vitamin B3 and NAD+ precursor, is converted to bioavailable NAD+, via nicotinamide riboside kinase (NRK) and NMNAT, or by the action of nucleoside phosphorylase and NAM salvage[1-2].
In in vitro experiments ,nicotinamide riboside attenuates alcohol induced liver injuries via activation of SirT1/PGC-1α/mitochondrial biosynthesis pathway [3]. Nicotinamide Riboside Enhances In Vitro Beta-adrenergic Brown Adipose Tissue Activity in Humans[4].
Nicotinamide Riboside was able to enhance the skeletal muscle NAD+ metabolome, inducing gene expression signatures implicated in downregulation of energy metabolism pathways, but did not affect muscle mitochondrial bioenergetics or metabolism[5]. Nicotinamide Riboside enhances deacetylase activity in vivo, deacetylates PGC-1α in muscle, liver, and BAT, and it induces deacetylase activity in tissues where NAD+ accumulates[6].
References:
[1] Bieganowski P., Brenner C. Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans. Cell.2004;117:495–502.
[2] Nikiforov A., Dolle C., Niere M. Pathways and subcellular compartmentation of NAD biosynthesis in human cells: from entry of extracellular precursors to mitochondrial NAD generation. J. Biol. Chem. 2011;286:21767–21778.
[3] Wang S, Wan T, et al. Nicotinamide riboside attenuates alcohol induced liver injuries via activation of SirT1/PGC-1α/mitochondrial biosynthesis pathway. Redox Biol. 2018 Jul;17:89-98.
[4] Nascimento EBM, Moonen MPB, et al. Nicotinamide Riboside Enhances In Vitro Beta-adrenergic Brown Adipose Tissue Activity in Humans. J Clin Endocrinol Metab. 2021 Apr 23;106(5):1437-1447.
[5] Elhassan YS, Kluckova K, et al. Nicotinamide Riboside Augments the Aged Human Skeletal Muscle NAD+ Metabolome and Induces Transcriptomic and Anti-inflammatory Signatures. Cell Rep. 2019 Aug 13;28(7):1717-1728.e6.
[6] Cantó C, Houtkooper RH,et al. The NAD(+) precursor nicotinamide riboside enhances oxidative metabolism and protects against high-fat diet-induced obesity. Cell Metab. 2012 Jun 6;15(6):838-47.
| Cell experiment [1]: | |
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Cell lines |
Murine RAW 264.7 macrophages |
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Preparation Method |
The prepared mouse bone marrow-derived macrophages were cultured and stored at 37°C in 5% CO2. Murine RAW 264.7 macrophages were treated with EtOH (80 mM), MeCHO (200 μM), and nicotinamide riboside (1 mM), respectively, and ROS accumulation was determined. |
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Reaction Conditions |
1 mM ,72 h |
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Applications |
Ethanol and its metabolite, acetaldehyde, significantly increased cellular ROS levels, but Nicotinamide riboside completely abolished the increase to a basal level in RAW 264.7 macrophages. |
| Animal experiment [2]: | |
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Animal models |
Eight-week-old male C57BL/6 J mice |
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Preparation Method |
The reared mice were randomly divided into 3 groups: control group (CTRL), ethanol group (EtOH) and nicotinamide riboside supplementation group (EtOH+ Nicotinamide riboside ). Mice in the ethanol and nicotinamide riboside supplemented groups were fed a Lieber-DeCarli ethanol liquid diet, while control mice were paired as previously described. |
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Dosage form |
400 mg/kg, oral gavage once daily for 10 consecutive days |
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Applications |
Mice were fed in pairs and there was no difference in body weight between the three groups. Fat accumulation was observed in the ethanol group, while only a few tiny lipid droplets were observed in the nicotinamide riboside group. Nicotinamide riboside significantly decreased serum ALT and AST and liver triglyceride levels, and slightly decreased liver weight ratio. |
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References: [1]. Wang S, Wan T,et al. Nicotinamide riboside attenuates alcohol induced liver injuries via activation of SirT1/PGC-1α/mitochondrial biosynthesis pathway. Redox Biol. 2018 Jul;17:89-98. [2]. Kang H, Park YK, Lee JY. Nicotinamide riboside, an NAD+ precursor, attenuates inflammation and oxidative stress by activating sirtuin 1 in alcohol-stimulated macrophages. Lab Invest. 2021 Sep;101(9):1225-1237. |
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| Cas No. | 1341-23-7 | SDF | |
| Canonical SMILES | NC(C1=C[N+]([C@H]2[C@H](O)[C@H](O)[C@@H](CO)O2)=CC=C1)=O | ||
| Formula | C11H15N2O5 | M.Wt | 255.3 |
| الذوبان | Water:125mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.917 mL | 19.5848 mL | 39.1696 mL |
| 5 mM | 783.4 μL | 3.917 mL | 7.8339 mL |
| 10 mM | 391.7 μL | 1.9585 mL | 3.917 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 16 reference(s) in Google Scholar.)
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