Podophyllotoxin-d6 (Synonyms: PPT-d6) |
| رقم الكتالوجGC49077 |
An internal standard for the quantification of podophyllotoxin and picropodophyllotoxin
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: N/A
Sample solution is provided at 25 µL, 10mM.
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Podophyllotoxin-d6 is intended for use as an internal standard for the quantification of podophyllotoxin and picropodophyllotoxin by GC- or LC-MS. Podophyllotoxin is a lignan that has been found in Podophyllum and has diverse biological activities.1,2,3,4,5 It inhibits polymerization of isolated chicken brain tubulin (IC50 = 0.6 µM) and induces mitotic arrest at the G2/M phase in CCRF CEM lymphoblastic leukemia cells when used at concentrations ranging from 0.01 to 5 µg/ml.2,3 Podophyllotoxin (5 µM) is protective against herpes simplex virus 1 (HSV-1) infection in Vero cells.4 It inhibits the proliferation of several diffuse large B cell lymphoma (DLBCL) cell lines (IC50s = 0.0098-0.017 µM).5 Podophyllotoxin is a starting material in the semisynthesis of the anticancer compounds etoposide and teniposide .6 Formulations containing podophyllotoxin have been used in the treatment of external genital warts.
1.Imbert, T.F.Discovery of podophyllotoxinsBiochimie80(3)207-222(1998) 2.Loike, J.D., Brewer, C.F., Sternlicht, H., et al.Structure-activity study of the inhibition of microtubule assembly in vitro by podophyllotoxin and its congenersCancer Res.38(9)2688-2693(1978) 3.Krishan, A., Paika, K., and Frei, E., IIICytofluorometric studies on the action of podophyllotoxin and epipodophyllotoxins (VM-26, VP-16-213) on the cell cycle traverse of human lymphoblastsJ. Cell Biol.66(3)521-530(1975) 4.Hammonds, T.R., Denyer, S.P., Jackson, D.E., et al.Studies to show that with podophyllotoxin the early replicative stages of herpes simplex virus type 1 depend upon functional cytoplasmic microtubulesJ. Med. Microbiol.45(3)167-172(1996) 5.StrÖmberg, T., Feng, X., Delforoush, M., et al.Picropodophyllin inhibits proliferation and survival of diffuse large B-cell lymphoma cellsMed. Oncol.32(7)188(2015) 6.Xu, H., Lv, M., and Tian, X.A review on hemisynthesis, biosynthesis, biological activities, mode of action, and structure-activity relationship of podophyllotoxins: 2003-2007Curr. Med. Chem.16(3)327-349(2009)
| Cas No. | N/A | SDF | |
| المرادفات | PPT-d6 | ||
| Canonical SMILES | O=C1[C@]2([H])[C@@](C3=CC(OC([2H])([2H])[2H])=C(C(OC)=C3)OC([2H])([2H])[2H])([H])C4=CC(OCO5)=C5C=C4[C@@H]([C@@]2([H])CO1)O | ||
| Formula | C22H16D6O8 | M.Wt | 420.4 |
| الذوبان | Acetonitrile: soluble,DMF: soluble,DMSO: soluble,Ethanol: soluble,Methanol: soluble | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3787 mL | 11.8934 mL | 23.7869 mL |
| 5 mM | 0.4757 mL | 2.3787 mL | 4.7574 mL |
| 10 mM | 0.2379 mL | 1.1893 mL | 2.3787 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 4 reference(s) in Google Scholar.)
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