الصفحة الرئيسية>>Signaling Pathways>> Cell Cycle/Checkpoint>> Cytoskeleton & Motor Proteins>>(S)-3'-amino Blebbistatin

(S)-3'-amino Blebbistatin (Synonyms: (-)-3'-amino Blebbistatin, m-amino Blebbistatin, meta-amino Blebbistatin)

رقم الكتالوجGC41557

(S)-3'-amino Blebbistatin is a more stable and less phototoxic form of (-)-blebbistatin, which is a selective cell-permeable inhibitor of non-muscle myosin II ATPases.

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(S)-3'-amino Blebbistatin التركيب الكيميائي

Cas No.: 2097141-18-7

الحجم السعر المخزون الكميّة
500μg
170٫00
متوفر
1mg
321٫00
متوفر
5mg
1526٫00
متوفر

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Sample solution is provided at 25 µL, 10mM.

Description of (S)-3'-amino Blebbistatin

(S)-3'-amino Blebbistatin is a more stable and less phototoxic form of (-)-blebbistatin , which is a selective cell-permeable inhibitor of non-muscle myosin II ATPases. (-)-Blebbistatin rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC50s = 0.5-5 µM), while poorly inhibiting smooth muscle myosin (IC50 = 80 µM). Through these effects, it blocks apoptosis-related bleb formation, directed cell migration, and cytokinesis in vertebrate cells. However, prolonged exposure to blue light (450-490 nm) results in degradation of blebbistatin to an inactive product via cytotoxic intermediates, which may be problematic for its use in fluorescent live cell imaging applications. The addition of a 3'-amino group decreases the inherent fluorescence while retaining the activity of (-)-blebbistatin. (S)-3'-amino Blebbistatin has the same stereochemistry as the active (-)-blebbistatin enantiomer.

Chemical Properties of (S)-3'-amino Blebbistatin

Cas No. 2097141-18-7 SDF
المرادفات (-)-3'-amino Blebbistatin, m-amino Blebbistatin, meta-amino Blebbistatin
Canonical SMILES O=C1[C@@]2(O)C(N(C3=CC=CC(N)=C3)CC2)=NC4=C1C=C(C)C=C4
Formula C18H17N3O2 M.Wt 307.3
الذوبان DMF: 20 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 12.5 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of (S)-3'-amino Blebbistatin

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1 mg 5 mg 10 mg
1 mM 3.2541 mL 16.2707 mL 32.5415 mL
5 mM 0.6508 mL 3.2541 mL 6.5083 mL
10 mM 0.3254 mL 1.6271 mL 3.2541 mL
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In vivo Formulation Calculator (Clear solution) of (S)-3'-amino Blebbistatin

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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