S-Adenosyl-L-methionine disulfate tosylate (Synonyms: AdoMet, FO 1561, SAM, SAMe) |
| رقم الكتالوجGC30843 |
S-Adenosyl-L-methionine disulfate tosylate (Ademetionine disulfate tosylate) هو المتبرع البيولوجي الرئيسي للميثيل الذي تم تصنيعه في جميع خلايا الثدييات ولكن بكثرة في الكبد
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 97540-22-2
Sample solution is provided at 25 µL, 10mM.
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S-Adenosyl-L-methionine disulfate tosylate is a methyl donor and cofactor. It is involved in a variety of biological processes, including epigenetic regulation, post-translational modification of proteins and metabolism. Preparations containing S-Adenosyl-L-methionine disulfate tosylate have been used as dietary supplements. S-Adenosyl-L-methionine disulfate tosylate also exhibits antiproliferative, pro-apoptotic and anti-metastatic effects in human cancers[1-2].
S-Adenosyl-L-methionine disulfate tosylate (300 μM) can regulate the cell cycle progression of Cal-33 and JHU-SCC-011 cells and reduce the expression of cyclins B1, E1, and D1. S-Adenosyl-L-methionine disulfate tosylate (200 μM and 300 μM) can inhibit the migration of Cal-33 and JHU-SCC-011 cells in a dose-dependent manner after 24 hours and 48 hours, respectively[2]. The combination of 5‑fluorouracil (5-FU) with 5-Adenosyl-L-methionine disulfate tosylate at 5-40 μg/ml can protect the anticancer effect of 5-FU by regulating the expression of DNA methyltransferases (DNMTs)[3].
In the elevated plus maze test, rats given S-Adenosyl-L-methionine disulfate tosylate (100 mg/kg) showed the greatest reduction in transfer latency at day 26, confirming the memory-improving effect of S-Adenosyl-L-methionine disulfate tosylate. Animals treated with 100 mg/kg S-Adenosyl-L-methionine disulfate tosylate showed decreased malondialdehyde and increased glutathione levels, suggesting that S-Adenosyl-L-methionine disulfate tosylate has antioxidant properties in the PTZ-induced rat model. The antioxidant properties of S-Adenosyl-L-methionine disulfate tosylate may improve cognitive impairment caused by oxidative stressl[4]. S-Adenosyl-L-methionine disulfate tosylate (30 mg/kg) can prevent autism spectrum disorder (ASD)-like behaviors caused by early postnatal valproic acid treatment in rat pups[5].
References:
[1] Loenen, W.A.M.S-Adenosylmethionine: Jack of all trades and master of everything?Biochem. Soc. Trans.34(2)330-333(2006).
[2] Mosca L, Minopoli M, Pagano M, Vitiello F, Carriero MV, Cacciapuoti G, Porcelli M. Effects of S-adenosyl-L-methionine on the invasion and migration of head and neck squamous cancer cells and analysis of the underlying mechanisms. Int J Oncol. 2020 May;56(5):1212-1224.
[3] Ham MS, et al. S-adenosyl methionine specifically protects the anticancer effect of 5-FU via DNMTs expression in human A549 lung cancer cells. Mol Clin Oncol. 2013 Mar;1(2):373-378.
[4] Dhediya RM, Joshi SS, Gajbhiye SV, Jalgaonkar SV, Biswas M. Evaluation of antiepileptic effect of S-adenosyl methionine and its role in memory impairment in pentylenetetrazole-induced kindling model in rats. Epilepsy Behav. 2016 Aug;61:153-157.
[5] Ornoy A, et al. S-adenosyl methionine prevents ASD like behaviors triggered by early postnatal valproic acid exposure in very young mice. Neurotoxicol Teratol. 2019 Jan-Feb;71:64-74.
| Cell experiment [1]: | |
Cell lines | Cal-33 and JHU-SCC-011 cells |
Preparation Method | Cal-33 and JHU-SCC-011 cells were treated with 300 μM S-Adenosyl-L-methionine disulfate tosylate and the cells were harvested after 24 and 48 hours, respectively, for flow cytometry and western blot analysis. |
Reaction Conditions | 300μM, 24h or 48h |
Applications | S-Adenosyl-L-methionine disulfate tosylate can regulate the cell cycle progression of Cal-33 and JHU-SCC-011 cells and reduce the expression of cyclins B1, E1 and D1. |
| Animal experiment [2]: | |
Animal models | Pentylenetetrazole (PTZ)-induced kindling model in rats |
Preparation Method | Out of 48 rats, 24 rats were used for standardization of the pentylenetetrazole (PTZ) kindling model. The remaining 24 rats were divided into 4 groups with 6 rats in each group as follows: 1. disease control: 0.9% normal saline 1 ml intraperitoneally (ip) + PTZ 35 mg/kg ip; 2. positive control: sodium valproate 200 mg/kg ip + PTZ 35 mg/kg ip; 3. S-Adenosyl-L-methionine disulfate tosylate 50 mg/kg oral + PTZ 35 mg/kg ip; 4. S-Adenosyl-L-methionine disulfate tosylate 100 mg/kg oral+ PTZ 35 mg/kg ip. |
Dosage form | 50 and 100 mg/kg, oral |
Applications | A higher dose of S-Adenosyl-L-methionine disulfate tosylate (100 mg/kg) exhibited an increase in seizure latency and a decrease in seizure severity score. |
References: [1] Mosca L, Minopoli M, Pagano M, Vitiello F, Carriero MV, Cacciapuoti G, Porcelli M. Effects of S-adenosyl-L-methionine on the invasion and migration of head and neck squamous cancer cells and analysis of the underlying mechanisms. Int J Oncol. 2020 May;56(5):1212-1224. [2] Dhediya RM, Joshi SS, Gajbhiye SV, Jalgaonkar SV, Biswas M. Evaluation of antiepileptic effect of S-adenosyl methionine and its role in memory impairment in pentylenetetrazole-induced kindling model in rats. Epilepsy Behav. 2016 Aug;61:153-157. | |
| Cas No. | 97540-22-2 | SDF | |
| المرادفات | AdoMet, FO 1561, SAM, SAMe | ||
| Canonical SMILES | N[C@@H](CC[S+](C[C@@H]1[C@H]([C@H]([C@H](N2C=NC3=C2N=CN=C3N)O1)O)O)C)C(O)=O.O=S(O)([O-])=O.O=S(O)(O)=O.OS(=O)(C4=CC=C(C)C=C4)=O | ||
| Formula | C22H34N6O16S4 | M.Wt | 766.8 |
| الذوبان | DMSO : ≥ 50 mg/mL (65.21 mM);Water : 33.33 mg/mL (43.47 mM) | Storage | Store at -20°C,protect from light, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.3041 mL | 6.5206 mL | 13.0412 mL |
| 5 mM | 0.2608 mL | 1.3041 mL | 2.6082 mL |
| 10 mM | 0.1304 mL | 0.6521 mL | 1.3041 mL |
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- Purity: >98.00%
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Average Rating: 5 (Based on Reviews and 33 reference(s) in Google Scholar.)
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