BMP signaling agonist sb4 |
رقم الكتالوجGC50593 |
ناهض إشارات BMP sb4 عبارة عن ناهض للإشارات من بروتين بنزوكسازول لتشكيل العظام 4 (BMP4) بقيمة EC50 تبلغ 74 نانومتر ، وينشط إشارات BMP عن طريق تثبيت p-SMAD-1/5/9 داخل الخلاياناهض إشارات BMP sb4 ينشط الجينات المستهدفة BMP4 (مثبطات ربط الحمض النووي ، Id1 و Id3) إشارات BMP الأساسية
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Cas No.: 100874-08-6
Sample solution is provided at 25 µL, 10mM.
BMP signaling agonist sb4 is a potent benzoxazole bone morphogenetic protein 4 (BMP4) signaling agonist with a EC50 value of 74 nM, activates BMP signaling by stabilizing intracellular p-SMAD-1/5/9. BMP signaling agonist sb4 activates BMP4 target genes (inhibitors of DNA binding, Id1 and Id3) canonical BMP signaling[1].
BMP signaling agonist sb4 (0.05 μM-1 μM; 24 hours) induces phosphorylated SMAD-1/5/9 in a dose-dependent manner in PRECs cells under serum-starved conitions[1]. BMP signaling agonist sb4 (1 μM; 0-60 mins) enhances the efficacy of signaling at each concentration of rhBMP4 tested. This effect is most pronounced at low concentrations of rhBMP4, whereby sb4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4[1].
Reference:
[1]. Bradford STJ, et al. High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds.J Biol Chem. 2019 Mar 1;294(9):3125-3136.
Cas No. | 100874-08-6 | SDF | |
Canonical SMILES | BrC1=CC=C(C=C1)CSC2=NC3=C(C=CC=C3)O2 | ||
Formula | C14H10BrNOS | M.Wt | 320.2 |
الذوبان | Soluble in DMSO | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
1 mM | 3.123 mL | 15.6152 mL | 31.2305 mL |
5 mM | 0.6246 mL | 3.123 mL | 6.2461 mL |
10 mM | 0.3123 mL | 1.5615 mL | 3.123 mL |
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.50%
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Average Rating: 5
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