TBHQ (tert-Butylhydroquinone) |
| رقم الكتالوجGC34057 |
TBHQ (ثلاثي بوتيل هيدروكينون) (ثلاثي بوتيل هيدروكينون) هو منشط Nrf2 واسع الاستخدام ، يحمي من السمية القلبية التي يسببها دوكسوروبيسين (DOX) من خلال تنشيط Nrf2. TBHQ (ثلاثي بوتيل هيدروكينون) (ثلاثي بوتيل هيدروكينون) هو أيضًا منشط ERK ؛ ينقذ تثبيط تكاثر الخلايا الناجم عن Dehydrocorydaline (DHC) في سرطان الجلد.
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Cas No.: 1948-33-0
Sample solution is provided at 25 µL, 10mM.
TBHQ(tert-Butylhydroquinone) is a powerful phenolic antioxidant capable of reducing oxidative stress and inflammatory reactions. It triggers the activation of Nrf2 and is recognized for its anti-inflammatory, anti-apoptotic, and neuroprotective properties[1-3].
TBHQ(tert-Butylhydroquinone) treatment(Pre-treating with 40μM) for 16 hours reduced paraquat (PQ)-induced apoptosis in cells [4]. TBHQ(tert-Butylhydroquinone) inhibits apoptosis and mitigates oxidative stress by activating the Nrf2/HO-1 pathway in HepG2 cells[5].
TBHQ(tert-Butylhydroquinone) (16.7mg/kg/d;daily for 5 days ;i.p) markedly ameliorated depression-like behavior induced by LPS and suppressed inflammatory responses along with microglial activation in mice [6]. TBHQ(tert-Butylhydroquinone) (50 mg/kg body weight in 1% DMSO; p.o; 14days) shields the testicular tissue and maintains steroidogenesis and spermatogenesis in rats treated with DOX by suppressing oxidative stress, inflammation, and apoptosis [7].
References:
[1]. Lu XY, Wang HD, et,al. Pretreatment with tert-butylhydroquinone attenuates cerebral oxidative stress in mice after traumatic brain injury. J Surg Res. 2014 May 1;188(1):206-12. doi: 10.1016/j.jss.2013.11.1106. Epub 2013 Dec 1. PMID: 24387843.
[2]. Bai J, Yu XJ, et,al. Tert-butylhydroquinone attenuates oxidative stress and inflammation in hypothalamic paraventricular nucleus in high salt-induced hypertension. Toxicol Lett. 2017 Nov 5;281:1-9. doi: 10.1016/j.toxlet.2017.08.018. Epub 2017 Aug 24. PMID: 28844481.
[3]. Sukumari-Ramesh S, Alleyne CH Jr. Post-Injury Administration of Tert-butylhydroquinone Attenuates Acute Neurological Injury After Intracerebral Hemorrhage in Mice. J Mol Neurosci. 2016 Apr;58(4):525-31. doi: 10.1007/s12031-016-0722-y. Epub 2016 Feb 11. PMID: 26867538.
[4]. Li H, Wu S, et,al. Neuroprotective effects of tert-butylhydroquinone on paraquat-induced dopaminergic cell degeneration in C57BL/6 mice and in PC12 cells. Arch Toxicol. 2012 Nov;86(11):1729-40. doi: 10.1007/s00204-012-0935-y. Epub 2012 Sep 15. PMID: 22983789.
[5]. Li R, Zhang P, et,al. Tert-butylhydroquinone mitigates Carbon Tetrachloride induced Hepatic Injury in mice. Int J Med Sci. 2020 Jul 29;17(14):2095-2103. doi: 10.7150/ijms.45842. PMID: 32922170; PMCID: PMC7484658.
[6]. Bian H, Yan F, et,al.Tert-butylhydroquinone prevents neuroinflammation and relieves depression via regulation of NLRP3 signaling in mice. Int Immunopharmacol. 2022 Jun;107:108723. doi: 10.1016/j.intimp.2022.108723. Epub 2022 Mar 23. PMID: 35338961.
[7]. Ujah GA, Nna VU, et,al. Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats. Sci Rep. 2021 Mar 9;11(1):5522. doi: 10.1038/s41598-021-85026-7. PMID: 33750916; PMCID: PMC7970903.
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Cell experiment [1]: |
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Cell lines |
PC12 cells |
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Preparation method |
PC12 cells were incubated with or without 40 μM Tert-butylhydroquinone for 16 hours, followed by exposure to 100 / 300 μM paraquat (PQ) for 24 hours. |
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Reaction Conditions |
40 μM;16 h |
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Applications |
TBHQ(tert-Butylhydroquinone) pretreatment had protective effect on PQ-induced apoptosis. |
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Animal experiment [2]: |
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Animal models |
C57BL/6J male mice (weight 20-25g) |
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Preparation method |
LPS was administered intraperitoneally at a daily dose of 1 mg/kg for five consecutive days. TBHQ(tert-Butylhydroquinone), dissolved in dimethyl sulfoxide and then diluted with PBS, was injected intraperitoneally at a daily dose of 16.7 mg/kg for five days. |
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Dosage form |
16.7mg/kg/d;daily for 5 days ;i.p |
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Applications |
TBHQ(tert-Butylhydroquinone) ameliorated LPS-induced caspase-1 expression in mice. |
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References: [1]. Li H, Wu S, et,al. Neuroprotective effects of tert-butylhydroquinone on paraquat-induced dopaminergic cell degeneration in C57BL/6 mice and in PC12 cells. Arch Toxicol. 2012 Nov;86(11):1729-40. doi: 10.1007/s00204-012-0935-y. Epub 2012 Sep 15. PMID: 22983789. [2]. Bian H, Yan F, et,al. Tert-butylhydroquinone prevents neuroinflammation and relieves depression via regulation of NLRP3 signaling in mice. Int Immunopharmacol. 2022 Jun;107:108723. doi: 10.1016/j.intimp.2022.108723. Epub 2022 Mar 23. PMID: 35338961. |
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| Cas No. | 1948-33-0 | SDF | |
| Canonical SMILES | OC1=CC=C(O)C=C1C(C)(C)C | ||
| Formula | C10H14O2 | M.Wt | 166.22 |
| الذوبان | DMSO : ≥ 56.66 mg/mL (340.87 mM) | Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.0161 mL | 30.0806 mL | 60.1612 mL |
| 5 mM | 1.2032 mL | 6.0161 mL | 12.0322 mL |
| 10 mM | 0.6016 mL | 3.0081 mL | 6.0161 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 6 reference(s) in Google Scholar.)
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