Vimentin (139-159)-biotin Peptide (Synonyms: VIM (139-159)-biotin) |
| رقم الكتالوجGC52364 |
A biotinylated vimentin peptide
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Vimentin (139-159)-biotin peptide is a biotinylated version of the cytoskeletal intermediate filament protein vimentin, which undergoes citrullination and has been found as the citrullinated form in the synovial fluid of rheumatoid arthritis patients.1 Vimentin is subject to citrullination by peptidyl arginine deiminase 2 (PAD2) under high calcium concentrations, which can occur during macrophage apoptosis.2,3 's Vimentin (139-159)-biotin Peptide is intended for use as a negative control in the detection of autoantibodies against citrullinated vimentin and is the non-citrullinated form of Citrullinated Vimentin (R144) (139-159)-biotin Peptide .
1.Bang, H., Egerer, K., Gauliard, A., et al.Mutation and citrullination modifies vimentin to a novel autoantigen for rheumatoid arthritisArthritis Rheum.56(8)2503-2511(2007) 2.Li, F.J., Surolia, R., Li, H., et al.Citrullinated vimentin mediates development and progression of lung fibrosisSci. Transl. Med.13(585)eaba2927(2021) 3.Asaga, H., Yamada, M., and Senshu, T.Selective deimination of vimentin in calcium ionophore-induced apoptosis of mouse peritoneal macrophagesBiochem. Biophys. Res. Commun.243(3)641-646(1998)
| Cas No. | SDF | ||
| المرادفات | VIM (139-159)-biotin | ||
| Formula | M.Wt | 2777.0 | |
| الذوبان | Storage | -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.3601 mL | 1.8005 mL | 3.601 mL |
| 5 mM | 0.072 mL | 0.3601 mL | 0.7202 mL |
| 10 mM | 0.036 mL | 0.1801 mL | 0.3601 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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