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Atrial Natriuretic Peptide (ANP) (1-28), human, porcine (Synonyms: ANF, ANP, Atrial Natriuretic Factor, α-Atriopeptin (human))

Catalog No.GC34023

Atrial Natriuretic Peptide (ANP) (1-28), human, porcine (Atrial Natriuretic Peptide (ANP) (1-28), human, porcine) is a 28-amino acid hormone, that is normally produced and secreted by the human heart in response to cardiac injury and mechanical stretch.

Products are for research use only. Not for human use. We do not sell to patients.

Atrial Natriuretic Peptide (ANP) (1-28), human, porcine Chemical Structure

Cas No.: 89213-87-6

Size Price Stock Qty
500μg
$166.00
In stock
1mg
$258.00
In stock
5mg
$901.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Animal experiment [1]:

Animal models

Female beagle dogs weighing 10–14 kg

Protocol

Protocol: Effects of carperitide on coronary vasodilation in nonischemic hearts.

Five dogs were used. Coronary arterial and venous blood was sampled for blood gas analysis. Myocardial oxygen consumption (ml per 100 g per min) is calculated by coronary blood flow (CBF) (ml per 100 g per min) the oxygen difference between coronary arterial and venous blood (ml dl-1). Measured coronary perfusion pressure (CPP) and CBF after dogs were randomly administered carperitide at a dose of 0.025, 0.05, 0.1 or 0.2 μg kg-1 min-1 into the left anterior descending coronary artery (LAD).

Dosage form

0.025, 0.05, 0.1 or 0.2 μg kg-1 min-1

Applications

CBF increased based on the dose of carperitide, with saturation of coronary vasodilation at a dose of 0.1μg kg1 min1despite no changes in CPP (102±2 mm Hg) or myocardial oxygen consumption.

References:

[1]. Asanuma H, Sanada S, et,al. Carperitide induces coronary vasodilation and limits infarct size in canine ischemic hearts: role of NO. Hypertens Res. 2014 Aug;37(8):716-23. doi: 10.1038/hr.2014.70. Epub 2014 Apr 3. PMID: 24694647.

Background

Atrial Natriuretic Peptide (ANP) (1-28), human, porcine (Carperitide) is a hormone containing 28 amino acids that is produced and secreted by the heart in response to cardiac injury and mechanical stretching[1]. Carperitide is widely used in patients with Heart failure(HF)[2].

Carperitide(1 mM;10min)significantly reduced infarct size in isolated rat hearts[3]. Carperitide inhibits immunoreactive (ir)-endothelin-1 secretion and increases cyclic GMP in the human umbilical-vein endothelial cells[4].

Carperitide dose increased, coronary blood flow (CBF) increased, and coronary artery diastole reached saturation at 0.1µg kg -1 min -1, but there was no change in CPP or myocardial oxygen consumption in dog[5].

References:
[1]. Kohno M, Yokokawa K, et,al. Atrial and brain natriuretic peptides inhibit the endothelin-1 secretory response to angiotensin II in porcine aorta. Circ Res. 1992 Feb;70(2):241-7. doi: 10.1161/01.res.70.2.241. PMID: 1310444.
[2]. Mukoyama M, Nakao K, et al. Brain natriuretic peptide as a novel cardiac hormone in humans. Evidence for an exquisite dual natriuretic peptide system, atrial natriuretic peptide and brain natriuretic peptide. J Clin Invest. 1991 Apr;87(4):1402-12. doi: 10.1172/JCI115146. PMID: 1849149; PMCID: PMC295184.
[3]. Okawa H, Horimoto H, et,al.Preischemic infusion of alpha-human atrial natriuretic peptide elicits myoprotective effects against ischemia reperfusion in isolated rat hearts. Mol Cell Biochem. 2003 Jun;248(1-2):171-7. doi: 10.1023/a:1024148621505. PMID: 12870670.
[4].Kohno M, Yasunari K, et,al. Inhibition by atrial and brain natriuretic peptides of endothelin-1 secretion after stimulation with angiotensin II and thrombin of cultured human endothelial cells. J Clin Invest. 1991 Jun;87(6):1999-2004. doi: 10.1172/JCI115228. PMID: 1645748; PMCID: PMC296954.
[5].Asanuma H, Sanada S, et,al.Carperitide induces coronary vasodilation and limits infarct size in canine ischemic hearts: role of NO. Hypertens Res. 2014 Aug;37(8):716-23. doi: 10.1038/hr.2014.70. Epub 2014 Apr 3. PMID: 24694647.

Chemical Properties

Cas No. 89213-87-6 SDF
Synonyms ANF, ANP, Atrial Natriuretic Factor, α-Atriopeptin (human)
Canonical SMILES Ser-Leu-Arg-Arg-Ser-Ser-Cys-Phe-Gly-Gly-Arg-Met-Asp-Arg-Ile-Gly-Ala-Gln-Ser-Gly-Leu-Gly-Cys-Asn-Ser-Phe-Arg-Tyr (Disulfide bridge: Cys7-Cys23)
Formula C127H203N45O39S3 M.Wt 3080.44
Solubility Water: 1 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 0.3246 mL 1.6231 mL 3.2463 mL
5 mM 0.0649 mL 0.3246 mL 0.6493 mL
10 mM 0.0325 mL 0.1623 mL 0.3246 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 15 reference(s) in Google Scholar.)

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