BAM-22P (8-22) (human, mouse, rat, bovine) (trifluoroacetate salt) (Synonyms: BAM 8-22, Bovine Adrenal Medulla 8-22) |
Catalog No.GC52409 |
A neuropeptide and MRGPRX1 agonist
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
BAM-22P (8-22) is an endogenous neuropeptide derived from proenkephalin A and an agonist of MAS-related G protein-coupled receptor family member X1 (MRGPRX1), previously known as sensory neuron-specific G protein-coupled receptor 4 (SNSR4).1 It is involved in prurition and nociception. BAM-22P (8-22) induces calcium mobilization in HEK293S cells expressing human MRGPRX1 or SNSR3 (EC50s = 14 and 28 nM, respectively) and inhibits voltage-induced calcium currents in isolated rat neurons expressing human MRGPRX1 (EC50 = 0.6 µM).2 Intrathecal administration of BAM-22P (8-22) (30 nmol/animal) decreases the tail-flick latency in rats stimulated with the Gαi inhibitor pertussin toxin and increases the tail-flick latency in rats stimulated with the phospholipase C (PLC) inhibitor U-73122, indicating changes in pain sensitivity.3 BAM-22P (8-22) (100 µg/animal) increases scratching behavior in wild-type mice and enhances bile-duct ligation-induced scratching behavior in a mouse model of cholestasis.4
1.Lembo, P.M.C., Grazzini, E., Groblewski, T., et al.Proenkephalin A gene products activate a new family of sensory neuron-specific GPCRsNat. Neurosci.5(3)201-209(2002) 2.Chen, H., and Ikeda, S.R.Modulation of ion channels and synaptic transmission by a human sensory neuron-specific G-protein-coupled receptor, SNSR4/mrgX1, heterologously expressed in cultured rat neuronsJ. Neurosci.24(21)5044-5053(2004) 3.Chen, T., Wang, D., and Hong, Y.Dual modulation effects of Mas-related gene (Mrg) receptors on pain sensitivity in ratsNeurosci. Lett.514(1)82-85(2012) 4.Sanjel, B., Maeng, H.-J., and Shim, W.-S.BAM8-22 and its receptor MRGPRX1 may attribute to cholestatic pruritusSci. Rep.9(1)10888(2019)
Cas No. | SDF | Download SDF | |
Synonyms | BAM 8-22, Bovine Adrenal Medulla 8-22 | ||
Canonical SMILES | OC(CNC([C@H](CC1=CC=C(O)C=C1)NC([C@H](CCCNC(N)=N)NC([C@H](CCCCN)NC([C@H](CCC(N)=O)NC([C@H](CC2=CC=C(O)C=C2)NC([C@H](CC(O)=O)NC([C@H](CCSC)NC([C@H](CC3=CNC4=CC=CC=C34)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@H]5N(CCC5)C([C@H](CCCNC(N)=N)NC(CNC([C@@H](N)C(C)C)=O)=O)=O)=O)=O)CC6=CNC7=CC=CC=C67)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O.OC(C(F)(F)F)=O | ||
Formula | C91H127N25O23S ? XCF3COOH | M.Wt | 1971.2 |
Solubility | Water: 1mg/ml | Storage | -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.5073 mL | 2.5365 mL | 5.0731 mL |
5 mM | 0.1015 mL | 0.5073 mL | 1.0146 mL |
10 mM | 0.0507 mL | 0.2537 mL | 0.5073 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
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