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BCA Protein Assay Kit

Catalog No.GK10009

BCA Protein Assay Kit is a ready-to-use total protein content determination kit with a detection range of 20-2000μg/mL.

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BCA Protein Assay Kit  Chemical Structure

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Sample solution is provided at 25 µL, 10mM.

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The BCA Protein Assay Kit is a ready-to-use total protein analysis reagent that can quickly determine total protein concentration by measuring the absorbance at 562 nm and comparing it with the absorbance-concentration curve of a protein standard.

This kit is suitable for measuring the total protein content of purified proteins, animal and plant tissue samples, and various cell samples. The detection concentration range is 20-2000 μg/mL. As the protein concentration increases, the absorbance has a linear relationship with the protein concentration.

This kit is developed based on the BCA (Bicin-choninic Acid) method, which enables fast, stable and sensitive concentration determination of protein. The principle is that under alkaline conditions, the protein reduces Cu2+ to Cu+, and Cu+ forms a purple complex with the BCA reagent. This water-soluble complex shows strong absorbance at 562 nm.

If you need to accurately quantify the protein to be tested, it is recommended to choose a protein standard with a quality similar to that of the protein to be tested. For example, when measuring immunoglobulin samples, bovine gamma globulin (BGG) standards can be used.

In addition, this kit provides a series of concentration gradient BSA (Bovine Serum Albumin, bovine serum albumin) standards with a concentration range of 125-2000μg/mL, eliminating the need for a series of gradient dilution processes and making the detection process faster.


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Average Rating: 5 ★★★★★ (Based on Reviews and 8 reference(s) in Google Scholar.)

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