BCECF (2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein) (Synonyms: 2',7'-bis(carboxyethyl)-5(6)-Carboxyfluorescein) |
| Catalog No.GC30270 |
BCECF (2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein) is a pH-sensitive fluorescent dye.
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Cas No.: 85138-49-4
Sample solution is provided at 25 µL, 10mM.
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BCECF is a duorescent dye with λexcitation of 420~520 nm and λemission of 530 nm.
Fully treated cells show hydrogenosomes with an electron-dense deposit which aggregates to a variable extent.The staining is seen in the interior of hydrogenosomes in some instances. It is also observed by microscopy that the K+/H+ ionophor nigericin does not inhibit hydrogenosomal loading with BCECF[1].
[1]. Scott DA, et al. Analysis of the uptake of the fluorescent marker 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) by hydrogenosomes in Trichomonas vaginalis. Eur J Cell Biol. 1998 Jun;76(2):139-45.
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