β-Casomorphin, human (Human β-casomorphin 7) (Synonyms: Human β-casomorphin 7) |
| Catalog No.GC33784 |
is an opioid peptide, acts as an agonist of opioid receptor.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 102029-74-3
Sample solution is provided at 25 µL, 10mM.
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β-Casomorphin, human is an opioid peptide, acts as an agonist of opioid receptor.
β-Casomorphin, human (TyrPro-Phe-Val-Glu-Pro-Ile) is also called human B-casein-(51-57), with the sequenceequivalent to bovine B-casein-(60-66) (Tyr-ProPhe-Pro-G]y-Pro-Ile or B-casomorphin-7)[1]. β-Casomorphin, human (100 μM) increases the expression of rat mucin (rMuc)2 and rMuc3 but not rMuc1, rMuc4, and rMuc5AC in rat DHE cells, also elevates increased MUC5AC mRNA levels[2].
[1]. Brantl V. Novel opioid peptides derived from human beta-casein: human beta-casomorphins. Eur J Pharmacol. 1984 Oct 30;106(1):213-4. [2]. Zoghbi S, et al. beta-Casomorphin-7 regulates the secretion and expression of gastrointestinal mucins through a mu-opioid pathway. Am J Physiol Gastrointest Liver Physiol. 2006 Jun;290(6):G1105-13.
Cell experiment: | To study the effect of β-Casomorphin, human and DAMGO, cells are seeded in 12-well culture plates. Experiments are performed 3 (DHE cells) or 21 days (HT29-MTX cells) after confluency. Twenty-four hours before the studies, the culture medium is replaced by serum-free medium to starve the cells from serum and to eliminate any interference from extraneous proteins or hormones. The experimental protocol is then the following: the serum-free medium is removed, and the monolayer cultures of DHE or HT29-MTX are washed twice with PBS (37°C). Serum-free medium with or without β-Casomorphin, human or DAMGO is added to the cells and incubated at 37°C for 30 min to 24 h in a humidified atmosphere. μ-Opioid receptor blockade is performed by preincubating the cells with cyprodime for 30 min before agonist addition. The supernatants are then collected, frozen, and stored at 20°C. Cells are processed with trypsin. The cell numbers per well are determined, and total RNA is isolated. All experiments are performed at least three times in triplicate[2]. |
References: [1]. Brantl V. Novel opioid peptides derived from human beta-casein: human beta-casomorphins. Eur J Pharmacol. 1984 Oct 30;106(1):213-4. | |
| Cas No. | 102029-74-3 | SDF | |
| Synonyms | Human β-casomorphin 7 | ||
| Canonical SMILES | Tyr-Pro-Phe-Val-Glu-Pro-Ile | ||
| Formula | C44H61N7O11 | M.Wt | 864 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.1574 mL | 5.787 mL | 11.5741 mL |
| 5 mM | 0.2315 mL | 1.1574 mL | 2.3148 mL |
| 10 mM | 0.1157 mL | 0.5787 mL | 1.1574 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >98.00%
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Average Rating: 5 (Based on Reviews and 39 reference(s) in Google Scholar.)
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