Biotin-16-dUTP trisodium |
| Catalog No.GC71740 |
Biotin-16- dUTP (Biotin-16-deoxyuridine-5'-triphosphate) trisodium can be used to replace its natural counterpart dTTP by enzymatically incorporating it into DNA/cDNA.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Biotin-16- dUTP trisodium can be used for in situ detection of DNA fragments by nick end labelling (TUNEL assay) for the study of apoptosis[1].
Biotin-16-dUTP trisodium can be used for replication foci labelling in permeabilised HeLa cell nuclei and detected by fluorescent streptavidin[2].
References:
[1]. C Charriaut-Marlangue, et al. A cautionary note on the use of the TUNEL stain to determine apoptosis. Neuroreport. 1995 Dec 29;7(1):61-4.
[2]. T Krude, et al. Chromatin assembly factor 1 (CAF-1) colocalizes with replication foci in HeLa cell nuclei. Exp Cell Res. 1995 Oct;220(2):304-11.
| Cas No. | SDF | ||
| Formula | C32H49N7Na3O18P3S | M.Wt | 1013.72 |
| Solubility | Storage | Solution, -20°C, protect from light, 2 years | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.9865 mL | 4.9323 mL | 9.8647 mL |
| 5 mM | 0.1973 mL | 0.9865 mL | 1.9729 mL |
| 10 mM | 0.0986 mL | 0.4932 mL | 0.9865 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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