Bleomycin A5 (hydrochloride) (Synonyms: Pingyangmycin) |
Catalog No.GC10909 |
glycopeptide antibiotic that lead to nucleic acid cleavage
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 55658-47-4
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >85.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Bleomycin A5 is an anticancer chemotherapeutic that binds DNA and induces DNA cleavage and strand breaks, it is highly toxic. Additionally, this compound induces apoptosis, alters cell cycle regulation, inhibits proliferation, and decreases tumor size in cellular and animal models of hemangioma. Bleomycin may also inhibit thioredoxin reductase.
In vitro: The IC50 of BLM alone to the cells was about 2.63 μg/ml. The IC50 of BLM was decreased to 1/3.8 and 1/9.5 of 2.63 μg/ml when used in combination with 2.63 μg/ml and 5 μg/ml W-131 [1]. Bleomycin A5 induced the activation of a JNK pathway in a dose-dependent manner. After 24h treatment, Bleomycin A5 induced dramatic cell death. DAPI nuclear staining assay revealed that PD98059 (10 μM) markedly enhanced bleomycin A5-induced apoptosis [2].
In vivo: In the rats, Bleomycin A5 induced pulmonary inflammation and fibrosis, increased the levels of malondialdehyde and tumour necrosis factor-α and enhanced accumulation of collagen in the lungs. Rolipram administration significantly attenuated these effects [3].
Clinical Trials: Bleomycin is clinically used to treat lymphomas, squamous cell carcinomas, and testicular cancer.
References:
[1]. Zhang H Q, He N G, Xue S B. Effect of bleomycin A5 with calmodulin inhibitor on the proliferation of S-180 cells in vitro[J]. Zhongguo yao li xue bao= Acta pharmacologica Sinica, 1990, 11(5): 470-473.
[2]. Yang L C, Yang S H, Tai K W, et al. MEK inhibition enhances bleomycin A5‐induced apoptosis in an oral cancer cell line: signaling mechanisms and therapeutic opportunities[J]. Journal of oral pathology & medicine, 2004, 33(1): 37-45.
[3]. Pan J B, Hou Y H, Zhang G J. Rolipram attenuates bleomycin A5‐induced pulmonary fibrosis in rats[J]. Respirology, 2009, 14(7): 975-982.
Cas No. | 55658-47-4 | SDF | |
Synonyms | Pingyangmycin | ||
Chemical Name | N1-[3-[(4-aminobutyl)amino]propyl]-bleomycinamide, hydrochloride | ||
Canonical SMILES | CC(C(N1)=N)=C(N=C1[C@](NC[C@@](N)([H])C(O)=N)([H])CC(O)=N)/C(O)=N/[C@@](/C(O)=N/[C@]([C@](O)([H])[C@@](/C(O)=N/[C@@](/C(O)=N/CCC2=NC(C3=NC(/C(O)=N/CCCNCCCCN)=CS3)=CS2)([H])[C@@](O)([H])C)([H])C)([H])C)([H])[C@](O[C@@]4([H])[C@](O[C@]5([H])[C@](O)([H])[C@] | ||
Formula | C57H89N19O21S2 • XHCl | M.Wt | 1477 |
Solubility | DMSO: 0.1 mg/mL,PBS (pH 7.2): 10 mg/mL | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.677 mL | 3.3852 mL | 6.7705 mL |
5 mM | 0.1354 mL | 0.677 mL | 1.3541 mL |
10 mM | 0.0677 mL | 0.3385 mL | 0.677 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
(Based on Reviews and 35 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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