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Bleomycin A5 (hydrochloride) (Synonyms: Pingyangmycin)

Catalog No.GC10909

glycopeptide antibiotic that lead to nucleic acid cleavage

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Bleomycin A5 (hydrochloride) Chemical Structure

Cas No.: 55658-47-4

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5mg
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10mg
$142.00
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25mg
$332.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Bleomycin A5 is an anticancer chemotherapeutic that binds DNA and induces DNA cleavage and strand breaks, it is highly toxic. Additionally, this compound induces apoptosis, alters cell cycle regulation, inhibits proliferation, and decreases tumor size in cellular and animal models of hemangioma. Bleomycin may also inhibit thioredoxin reductase.

In vitro: The IC50 of BLM alone to the cells was about 2.63 μg/ml. The IC50 of BLM was decreased to 1/3.8 and 1/9.5 of 2.63 μg/ml when used in combination with 2.63 μg/ml and 5 μg/ml W-131 [1]. Bleomycin A5 induced the activation of a JNK pathway in a dose-dependent manner. After 24h treatment, Bleomycin A5 induced dramatic cell death. DAPI nuclear staining assay revealed that PD98059 (10 μM) markedly enhanced bleomycin A5-induced apoptosis [2].

In vivo: In the rats, Bleomycin A5 induced pulmonary inflammation and fibrosis, increased the levels of malondialdehyde and tumour necrosis factor-α and enhanced accumulation of collagen in the lungs. Rolipram administration significantly attenuated these effects [3].

Clinical Trials: Bleomycin is clinically used to treat lymphomas, squamous cell carcinomas, and testicular cancer.

References:
[1].  Zhang H Q, He N G, Xue S B. Effect of bleomycin A5 with calmodulin inhibitor on the proliferation of S-180 cells in vitro[J]. Zhongguo yao li xue bao= Acta pharmacologica Sinica, 1990, 11(5): 470-473.
[2].  Yang L C, Yang S H, Tai K W, et al. MEK inhibition enhances bleomycin A5‐induced apoptosis in an oral cancer cell line: signaling mechanisms and therapeutic opportunities[J]. Journal of oral pathology & medicine, 2004, 33(1): 37-45.
[3].  Pan J B, Hou Y H, Zhang G J. Rolipram attenuates bleomycin A5‐induced pulmonary fibrosis in rats[J]. Respirology, 2009, 14(7): 975-982.

Chemical Properties

Cas No. 55658-47-4 SDF
Synonyms Pingyangmycin
Chemical Name N1-[3-[(4-aminobutyl)amino]propyl]-bleomycinamide, hydrochloride
Canonical SMILES CC(C(N1)=N)=C(N=C1[C@](NC[C@@](N)([H])C(O)=N)([H])CC(O)=N)/C(O)=N/[C@@](/C(O)=N/[C@]([C@](O)([H])[C@@](/C(O)=N/[C@@](/C(O)=N/CCC2=NC(C3=NC(/C(O)=N/CCCNCCCCN)=CS3)=CS2)([H])[C@@](O)([H])C)([H])C)([H])C)([H])[C@](O[C@@]4([H])[C@](O[C@]5([H])[C@](O)([H])[C@]
Formula C57H89N19O21S2 • XHCl M.Wt 1477
Solubility DMSO: 0.1 mg/mL,PBS (pH 7.2): 10 mg/mL Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 0.677 mL 3.3852 mL 6.7705 mL
5 mM 0.1354 mL 0.677 mL 1.3541 mL
10 mM 0.0677 mL 0.3385 mL 0.677 mL
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Reviews

Review for Bleomycin A5 (hydrochloride)

Average Rating: 5 ★★★★★ (Based on Reviews and 35 reference(s) in Google Scholar.)

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