BODIPY 505/515 |
| Catalog No.: GC42960 |
BODIPY 505/515, a lipophilic fluorescence dye, emits fluorescence has been used extensively for lipid droplet labeling (Ex/Em: 505/515 nm).
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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Purity: >98.00%
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- SDS (Safety Data Sheet)
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Procedure for BODIPY 505/515 staining of Aurantiochytrium sp. [1]
A 5 mM BODIPY 505/515 stock was prepared by dissolving in dimethyl sulfoxide and stored in the dark.
For efficient staining of the intracellular lipid bodies in Aurantiochytrium sp., an aliquot of 0.2% DMSO (vol/vol) was added to the microalgal suspension. 2 μL of BODIPY 505/515 solution was added into the l ml of algal-DMSO suspension and gently shaken for 1 min. Samples were then incubated in darkness for 5–10 min at room temperature. Then the samples were directly used for FACS and microscopic analyses
Procedure for BODIPY 505/515 staining of HepG2 and MCF7 cells. [2]
HepG2 and MCF7 cells were washed once with PBS and fixed in 4% paraformaldehyde for 15 min. Cells were washed three times with PBS and incubated with 0.2 μM BODIPY 505/515 for 30 min. The cells were then counterstained with 1.5 μg/mL DAPI for 5 min. Cells were examined and photographed using the fluorescence microscopy.
This protocol only provides a guideline, and should be modified according to your specific needs
References:
[1]. Velmurugan N, Sathishkumar Y, Yim S S, et al. Study of cellular development and intracellular lipid bodies accumulation in the thraustochytrid Aurantiochytrium sp. KRS101[J]. Bioresource technology, 2014, 161: 149-154.
[2]. Jhu J W, Yan J B, Lin Z H, et al. SREBP1-Induced Glutamine Synthetase Triggers a Feedforward Loop to Upregulate SREBP1 through Sp1 O-GlcNAcylation and Augments Lipid Droplet Formation in Cancer Cells[J]. International journal of molecular sciences, 2021, 22(18): 9814.
BODIPY 505/515, a lipophilic fluorescence dye, emits fluorescence has been used extensively for lipid droplet labeling (Ex/Em: 505/515 nm) [1，2] . BODIPY 505/515 is compatible with epifluorescent, confocal, and two-photon microscopy, and flow cytometry, and can be used for live and fixed cell applications. Bodipy 505/515 is used for labeling a broad variety of lipids, e.g., phospholipids, fatty acids, cholesteryl esters, cholesterol, and ceramides
References:
[1]. Velmurugan, N., Sathishkumar, Y., Yim, S.S., et al. Study of cellular development and intracellular lipid bodies accumulation in the thraustochytrid Aurantiochytrium sp. KRS101. Bioresour. Technol. 161, 149-154 (2014).
[2]. Rumin, J., Bonnefond, H., Saint-Jean, B., et al. The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae. Biotechnol. Biofuels 8, 42 (2015).
[3]. Elle I C, Olsen L C B, Pultz D, et al. Something worth dyeing for: molecular tools for the dissection of lipid metabolism in Caenorhabditis elegans[J]. FEBS letters, 2010, 584(11): 2183-2193.
| Cas No. | 21658-70-8 | SDF | |
| Chemical Name | (T-4)-[2-[(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-3,5-dimethyl-1H-pyrrolato-κN]difluoro-boron | ||
| Canonical SMILES | [F-][B+3]([N-]1C2=C(C)C=C1C)([N](C(C(C)=C3)=C2)=C3C)[F-] | ||
| Formula | C13H15BF2N2 | M.Wt | 248.1 |
| Solubility | 1.7mg/ml in DMSO; 25mg/ml in DMF | Storage | Store at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
| Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining
Bioresour Technol 2013 Jan;127:386-90.PMID:23138061DOI:10.1016/j.biortech.2012.09.068.
BODIPY 505/515, a lipophilic bright green fluorescent dye was tested for lipid detection in the microalga Tetraselmis subcordiformis. A concentration of 0.28 μg ml(-1) and staining for 6 min was optimal. Lipid bodies stained with BODIPY505/515 had a characteristic green fluorescence. Their volumes were determined using the sphere volume formula. Lipid accumulation under different nitrogen concentrations was analyzed. With an increase in NaNO(3) concentration from 0 to 240 mg L(-1), the maximum algal concentration increased from 8.23 ± 0.62 (× 10(5) cells ml(-1)) to 1.61 ± 0.13 (×10(6) cells ml(-1)), while the maximum volume of intracellular neutral lipid decreased from 9.78 ± 1.77 μm(3) cell(-1) to 6.00 ± 0.59 μm(3) cell(-1). A comparison of the lipid contents measured by BODIPY 505/515 staining and the gravimetric method showed a positive correlation coefficient of R(2) = 0.93. BODIPY 505/515 staining is a promising method in lipid quantitation in T. subcordiformis.
Confocal microscopic analysis of morphogenetic movements
Methods Cell Biol 1999;59:179-204.PMID:9891361DOI:10.1016/s0091-679x(08)61826-9.
Confocal microscopy is an excellent means of imaging cellular dynamics within living zebrafish embryos because it provides a means of optically sectioning tissues that have been labeled with specific fluorescent probe molecules. In order to study genetically encoded patterns of cell behavior that are involved in the formation of germ layers and various organ primordia, it is possible to vitally stain an entire zebrafish embryo with one or more fluorescent probe molecules and then examine morphogenetic behaviors within specific cell populations of interest using time-lapse confocal microscopy. There are two major advantages to this "bulk-labeling" approach: (1) the applied fluorescent probe (a contrast-enhancing agent) allows all of the cells within an intact zebrafish embryo to be rapidly stained; (2) the morphogenetic movements and shape changes of hundreds of cells can then be examined simultaneously in vivo using time-lapse confocal microscopy. The neutral fluorophore BODIPY 505/515 and its sphingolipid-derivative Bodipy-C5-ceramide are particularly useful, nonteratogenic vital stains for imaging cellular dynamics in living zebrafish embryos. These photostable fluorescent probes (when applied with 2% DMSO) percolate through the enveloping layer epithelium of the embryo, and localize in yolk-containing cytoplasm and interstitial space, respectively, owing to their different physiochemical characteristics. Bodipy-ceramide, for instance, remains highly localized to interstitial fluid once it accumulates within a zebrafish embryo, allowing the boundaries of deep cells to be clearly discerned throughout the entire embryo. Through the use of either of these fluorescent vital stains, it is possible to rapidly convert a developing zebrafish embryo into a strongly fluorescent specimen that is ideally suited for time-lapse confocal imaging. For zebrafish embryos whose deep cells have been intentionally "scatter-labeled" with fluorescent lineage tracers (e.g., fluorescent dextrans), sequential confocal z-series (i.e., focus-throughs) of the embryo can be rendered into uniquely informative 3D time-lapse movies using readily available image-processing programs. Similar time-lapse imaging, combined with rapidly advancing computer-assisted visualization techniques, may soon be applied to study the dynamics of GFP-fusion proteins in vivo, as well as other types of synthetic probe molecules designed to reveal the cytological processes associated with the patterning and morphological transformations of the zebrafish's embryonic tissues.
Advances in techniques for assessment of microalgal lipids
Crit Rev Biotechnol 2017 Aug;37(5):566-578.PMID:27417693DOI:10.1080/07388551.2016.1206058.
Microalgae are a varied group of organisms with considerable commercial potential as sources of various biochemicals, storage molecules and metabolites such as lipids, sugars, amino acids, pigments and toxins. Algal lipids can be processed to bio-oils and biodiesel. The conventional method to estimate algal lipids is based on extraction using solvents and quantification by gravimetry or chromatography. Such methods are time consuming, use hazardous chemicals and are labor intensive. For rapid screening of prospective algae or for management decisions (e.g. decision on timing of harvest), a rapid, high throughput, reliable, accurate, cost effective and preferably nondestructive analytical technique is desirable. This manuscript reviews the application of fluorescent lipid soluble dyes (Nile Red and BODIPY 505/515), nuclear magnetic resonance (NMR), Raman, Fourier transform infrared (FTIR) and near infrared (NIR) spectroscopy for the assessment of lipids in microalgae.
Evaluation of intracellular lipid bodies in Chlamydomonas reinhardtii strains by flow cytometry
Bioresour Technol 2013 Jun;138:30-7.PMID:23612159DOI:10.1016/j.biortech.2013.03.078.
A comparative study of Chlamydomonas reinhardtii wild type CC124 and a cell wall-less mutant sta6-1 is described using FACS in conjunction with two different lipophilic fluorescent dyes, Nile Red and BODIPY 505/515. The results indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies and single cell sorting than Nile Red. While BODIPY 505/515 stained cells continued to grow after single cell sorting using FACS, Nile Red stained cells failed to recover from sorting. In addition, a comprehensive study was performed to establish a quantitative baseline for future studies for either lipid accumulation and/or microalgal growth by measuring various parameters such as cell count, size, fatty acid contents/composition, and optical/confocal images of the wild type and mutant.
Visualizing "green oil" in live algal cells
J Biosci Bioeng 2010 Feb;109(2):198-201.PMID:20129108DOI:10.1016/j.jbiosc.2009.08.004.
We report here that BODIPY 505/515, a green lipophilic fluorescent dye, serves as an excellent vital stain for the oil-containing lipid bodies of live algal cells. BODIPY 505/515 vital staining can be used in combination with fluorescent activated cell sorting to detect and isolate algal cells possessing high lipid content.
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