Brassinolide |
| Catalog No.GC11632 |
A plant growth regulator
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 72962-43-7
Sample solution is provided at 25 µL, 10mM.
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IC50: N/A
Brassinolide is a plant growth regulator. Plant growth regulators are naturally produced by plants and are critical for regulating their own growth. Plant growth regulators act via modifying or controlling plant growth processes, such as leave and flower formation, stem elongation, as well as fruit development and ripening.
In vitro: Brassinolide induced the time and concentration-dependent cytotoxicity in PC-3 cells. The mode of such cell death appeared to be apoptosis predominately, as demonstrated by fluorescence, flow-cytometric analyses and transmission electron microscopes. Moreover, Caspase-3 activity was increased after brassinolide treatment obviously. Western blot studies showed brassinolide treatment triggered a time-dependent decrease in the expression of Bcl-2 [1].
In vivo: Brassinolide had the similar function of reducing the blood glucose levels as phenformin, but without the dose-dependent manner. The blood glucose levels showed significant differences after brassinolide treatment with different doses (200, 100, and 50 mg/kg). These results indicated that brassinolide could reduce the blood glucose levels without toxicity [2].
Clinical trial: So far, no clinical study has been reported.
References:
[1] Wu YD,Lou YJ. Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells. Pharmazie.2007 May;62(5):392-5.
[2] CHEN Shi-ping, HE Jia, WANG Qi-jing, WANG Jian-dong, YE Yan-li, XIAN Li-jian. Effect of Brassinolide on Levels of Blood Glucose in Alloxan—induced Diabetes Rats. Acta Metallurgica Sinica, 2009,V26(03): 21-23
| Cell experiment [1]: | |
|
Cell lines |
Human prostate cancer PC-3 cell |
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Preparation method |
The solubility of this compound in DMSO is >24.1mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
|
Reacting condition |
10, 20 and 40 μM; 12, 24 and 36 h |
|
Applications |
Brassinolide induced a time and concentration-dependent cytotoxicity in PC-3 cells. Brassinolide (10, 20 and 40 μM, 12 h) induced a concentration-dependent increase in the apoptotic rate and marked accumulation in G2/M phase of cell cycle. PC-3 cells treated with brassinolide (20 μM, 24 h) showed characteristic apoptotic alterations: shrinking cellular figure, decreasing cell surface microvilli, cytoplasmic vacuoles, chromatin condensation. PC-3 cells treated with brassinolide (20 μM) for 6, 12 and 18 h showed a time-dependent increase in the activity of caspases-3. |
| Animal experiment [2]: | |
|
Animal models |
Diabetes rats |
|
Dosage form |
Oral administration; 200,100,and 50 mg/kg; once every day for 7 days |
|
Application |
Oral administration of Brassinolide decreased the levels of blood glucose from 19.71-24.10 mmol/L to 9.89 mmol/L-12.70 mmol/L. The levels of blood glucose displayed significant differences after treatment with different dose of brassinolide. Brassinolide can still reduce the blood glucose levels without toxicity effect even at a lower dose. |
|
Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Wu Y D, Lou Y J. Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells[J]. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 2007, 62(5): 392-395. [2]. CHEN S, HE J, WANG Q, et al. Effect of Brassinolide on Levels of Blood Glucose in Alloxan—induced Diabetes Rats [J][J]. Laboratory Animal Science, 2009, 3: 007. |
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| Cas No. | 72962-43-7 | SDF | |
| Chemical Name | (3aS,5S,6R,7aR,7bS,9aS,10R,12aS,12bS)-10-((2S,3R,4R,5S)-3,4-dihydroxy-5,6-dimethylheptan-2-yl)-5,6-dihydroxy-7a,9a-dimethyltetradecahydro-1H-benzo[c]indeno[5,4-e]oxepin-3(12bH)-one | ||
| Canonical SMILES | CC(C)[C@H](C)[C@@H](O)[C@H](O)[C@@H](C)[C@@]1([H])CC[C@@]2([H])[C@]3([H])COC([C@@]4([H])C[C@H](O)[C@H](O)C[C@]4(C)[C@@]3([H])CC[C@@]21C)=O | ||
| Formula | C28H48O6 | M.Wt | 480.68 |
| Solubility | ≥ 48.1 mg/mL in DMSO with gentle warming, ≥ 52.3 mg/mL in EtOH with ultrasonic and warming | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0804 mL | 10.4019 mL | 20.8039 mL |
| 5 mM | 0.4161 mL | 2.0804 mL | 4.1608 mL |
| 10 mM | 0.208 mL | 1.0402 mL | 2.0804 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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