Bromazolam |
Catalog No.GC42980 |
Bromazolam is a triazolobenzodiazepine that is known as research chemical XLI-268.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 71368-80-4
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Kinase experiment [1]: | |
Preparation Method |
They were treated with pHLS9 (2 mg protein /mL), 25 μg/mL Alamethicin (UGT reaction mixture B), 90 mM phosphate buffer (pH 7.4), 2.5 mM mg 2+, 2.5 mM isocitrate, and 0.6 mM at 37°C NADP +, 0.9 U/mL isocitrate dehydrogenase, 100 U/mL superoxide dismutase and 0.1 mM acetyl-CoA. Then, 2.5 mM UDP-glucuronic acid (UGT reaction mixture solution A), 40 μM PAPS, 1.2 mM SAM, 1 mM dithiothreitol, 10 mM glutathione, and 50 μM clobromazolam or Bromazolam were added. |
Reaction Conditions |
50 μM Bromazolam for 360min in 37°C |
Applications |
Bromazolam metabolites were identified in pHLS9 incubation. |
References: [1]: Wagmann L, Manier SK, Felske C, Gampfer TM, Richter MJ, Eckstein N, Meyer MR. Flubromazolam-Derived Designer Benzodiazepines: Toxicokinetics and Analytical Toxicology of Clobromazolam and Bromazolam. J Anal Toxicol. 2021 Nov 9;45(9):1014-1027. doi: 10.1093/jat/bkaa161. PMID: 33048135. |
Bromazolam is a triazolobenzodiazepine that is known as research chemical XLI-268[1]. Bromazolam is structurally similar to alprazolam but is the bromo rather than the chloro analog[2]. Bromazolam metabolites were identified in pHLS9 incubation[3].Bromazolam (XLI-268) is a triazolobenzodiazepine (TBZD),It is the bromo instead of chloro analogue of alprazolam and has similar sedative and anxiolytic effects to it and other benzodiazepines.Bromazolam is a non subtype selective agonist at the benzodiazepine site of GABAA receptors, with a binding affinity of 2.81nM at the α1 subtype, 0.69nM at α2 and 0.62nM at α5.
References:
[1]: PubChem. 8-Bromo-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine. pubchem.ncbi.nlm.nih.gov website. Accessed August 18, 2021. https://pubchem.ncbi.nlm.nih.gov/compound/12562546
[2]: McDermott S, Johnson BE, Balasanova AA. Phenibut and Bromazolam Use Disorders Requiring Hospitalization for Medically Supervised Withdrawal. Prim Care Companion CNS Disord. 2022 Jul 12;24(4):21cr03119. doi: 10.4088/PCC.21cr03119. PMID: 35830740.
[3]: Wagmann L, Manier SK, Felske C, Gampfer TM, Richter MJ, Eckstein N, Meyer MR. Flubromazolam-Derived Designer Benzodiazepines: Toxicokinetics and Analytical Toxicology of Clobromazolam and Bromazolam. J Anal Toxicol. 2021 Nov 9;45(9):1014-1027. doi: 10.1093/jat/bkaa161. PMID: 33048135.
Cas No. | 71368-80-4 | SDF | |
Chemical Name | 8-bromo-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine | ||
Canonical SMILES | CC1=NN=C(N1C2=CC=C(Br)C=C23)CN=C3C4=CC=CC=C4 | ||
Formula | C17H13BrN4 | M.Wt | 353.2 |
Solubility | 5mg/mL in DMSO | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.8313 mL | 14.1563 mL | 28.3126 mL |
5 mM | 0.5663 mL | 2.8313 mL | 5.6625 mL |
10 mM | 0.2831 mL | 1.4156 mL | 2.8313 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
(Based on Reviews and 17 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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