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Bromazolam

Catalog No.GC42980

Bromazolam is a triazolobenzodiazepine that is known as research chemical XLI-268.

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Bromazolam Chemical Structure

Cas No.: 71368-80-4

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1mg
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5mg
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100mg
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500mg
$1,630.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Kinase experiment [1]:

Preparation Method

They were treated with pHLS9 (2 mg protein /mL), 25 μg/mL Alamethicin (UGT reaction mixture B), 90 mM phosphate buffer (pH 7.4), 2.5 mM mg 2+, 2.5 mM isocitrate, and 0.6 mM at 37°C NADP +, 0.9 U/mL isocitrate dehydrogenase, 100 U/mL superoxide dismutase and 0.1 mM acetyl-CoA. Then, 2.5 mM UDP-glucuronic acid (UGT reaction mixture solution A), 40 μM PAPS, 1.2 mM SAM, 1 mM dithiothreitol, 10 mM glutathione, and 50 μM clobromazolam or Bromazolam were added.

Reaction Conditions

50 μM Bromazolam for 360min in 37°C

Applications

Bromazolam metabolites were identified in pHLS9 incubation.

References:

[1]: Wagmann L, Manier SK, Felske C, Gampfer TM, Richter MJ, Eckstein N, Meyer MR. Flubromazolam-Derived Designer Benzodiazepines: Toxicokinetics and Analytical Toxicology of Clobromazolam and Bromazolam. J Anal Toxicol. 2021 Nov 9;45(9):1014-1027. doi: 10.1093/jat/bkaa161. PMID: 33048135.

Background

Bromazolam is a triazolobenzodiazepine that is known as research chemical XLI-268[1]. Bromazolam is structurally similar to alprazolam but is the bromo rather than the chloro analog[2]. Bromazolam metabolites were identified in pHLS9 incubation[3].Bromazolam (XLI-268) is a triazolobenzodiazepine (TBZD),It is the bromo instead of chloro analogue of alprazolam and has similar sedative and anxiolytic effects to it and other benzodiazepines.Bromazolam is a non subtype selective agonist at the benzodiazepine site of GABAA receptors, with a binding affinity of 2.81nM at the α1 subtype, 0.69nM at α2 and 0.62nM at α5.

References:
[1]: PubChem. 8-Bromo-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine. pubchem.ncbi.nlm.nih.gov website. Accessed August 18, 2021. https://pubchem.ncbi.nlm.nih.gov/compound/12562546
[2]: McDermott S, Johnson BE, Balasanova AA. Phenibut and Bromazolam Use Disorders Requiring Hospitalization for Medically Supervised Withdrawal. Prim Care Companion CNS Disord. 2022 Jul 12;24(4):21cr03119. doi: 10.4088/PCC.21cr03119. PMID: 35830740.
[3]: Wagmann L, Manier SK, Felske C, Gampfer TM, Richter MJ, Eckstein N, Meyer MR. Flubromazolam-Derived Designer Benzodiazepines: Toxicokinetics and Analytical Toxicology of Clobromazolam and Bromazolam. J Anal Toxicol. 2021 Nov 9;45(9):1014-1027. doi: 10.1093/jat/bkaa161. PMID: 33048135.

Chemical Properties

Cas No. 71368-80-4 SDF
Chemical Name 8-bromo-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine
Canonical SMILES CC1=NN=C(N1C2=CC=C(Br)C=C23)CN=C3C4=CC=CC=C4
Formula C17H13BrN4 M.Wt 353.2
Solubility 5mg/mL in DMSO Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.8313 mL 14.1563 mL 28.3126 mL
5 mM 0.5663 mL 2.8313 mL 5.6625 mL
10 mM 0.2831 mL 1.4156 mL 2.8313 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Average Rating: 5 ★★★★★ (Based on Reviews and 17 reference(s) in Google Scholar.)

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