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Bz 423 (BZ48) Catalog No.GC31806

Size Price Stock Qty
10mM*1mLinDMSO
$1,299.00
In stock
1mg
$268.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Quality Control

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Protocol

Kinase experiment:

To detect ASK1 activity, inactive GST-MKK6 (0.1 mg) is incubated (15 min, 25°C) with immunoprecipitated ASK1 in kinase assay buffer. Subsequently, GST-p38α (0.5 µg) is added to this reaction and incubated (15 min, 25°C) followed by detection of phospho-GST-p38 via immunoblot.

References:

[1]. Blatt NB, et al. Bz-423 superoxide signals B cell apoptosis via Mcl-1, Bak, and Bax. Biochem Pharmacol. 2009 Oct 15;78(8):966-73.
[2]. Blatt NB, et al. Bz-423 superoxide signals apoptosis via selective activation of JNK, Bak, and Bax. Free Radic Biol Med. 2008 Nov 1;45(9):1232-42.

Chemical Properties

Cas No. 216691-95-1 SDF Download SDF
Synonyms N/A
Chemical Name N/A
Canonical SMILES O=C1N(C)C2=CC=C(Cl)C=C2C(C3=CC=C(O)C=C3)=NC1CC4=CC=C5C=CC=CC5=C4
Formula C27H21ClN2O2 M.Wt 440.92
Solubility DMSO : ≥ 125 mg/mL (283.50 mM) Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
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Background

Bz 423 is a pro-apoptotic 1,4-benzodiazepine with therapeutic properties in murine models of lupus demonstrating selectivity for autoreactive lymphocytes, and activates Bax and Bak.

Bz-423 induces a larger superoxide response in Ramos cells, and causes rapid death of Ramos B cells. Bz-423 activates Bax and Bak, but does not activate ASK1/JNK in Ramos cells. In Ramos cells Bz-423-induced superoxide leads to Bax and Bak activation via changes in Mcl-1 expression and functional activation of BH3-only proteins[1]. Bz-423 rapidly increases superoxide levels in MEFs within 1 h and the magnitude of the increase is concentration-dependent. Bz-423-induced apoptosis dependents upon superoxide[2].

[1]. Blatt NB, et al. Bz-423 superoxide signals B cell apoptosis via Mcl-1, Bak, and Bax. Biochem Pharmacol. 2009 Oct 15;78(8):966-73. [2]. Blatt NB, et al. Bz-423 superoxide signals apoptosis via selective activation of JNK, Bak, and Bax. Free Radic Biol Med. 2008 Nov 1;45(9):1232-42.