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Caffeic Acid Phenethyl Ester (Synonyms: CAPE, 2Phenylethyl Caffeate, βPhenylethyl Caffeate)

Catalog No.GC10692

NF-κB activation inhibitor

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Caffeic Acid Phenethyl Ester Chemical Structure

Cas No.: 104594-70-9

Size Price Stock Qty
10mM (in 1mL DMSO)
$43.00
In stock
50mg
$52.00
In stock
200mg
$135.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

The human histiocytic cell line U937 cells

Preparation method

The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

0, 5, 10, 15, 25 and 30 μg/ml; 2 h

Applications

In U937 cells, Caffeic Acid Phenethyl Ester (CAPE) inhibited the TNF-dependent activation of NF-KB in a dose-dependent manner, with maximum effect occurring at 25 ug/ml. CAPE (25 ug/ml) also blocked NF-KB activation induced by Phorbol Ester, Ceramide, Okadaic Acid, and Hydrogen Peroxide.

Animal experiment [2]:

Animal models

CT26-bearing BALB/c male mice with pulmonary metastases

Dosage form

10 mg/kg/day, intraperitoneal injection

Application

In CT26-bearing BALB/c male mice with pulmonary metastases, CAPE decreased tumor colonization in the lung. Mice treated with CAPE survived longer than untreated controls. CAPE reduced plasma VEGF levels by 53.2%.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1] Natarajan K, Singh S, Burke TR Jr, Grunberger D, Aggarwal BB. Caffeic acid phenethyl ester is a potent and specific inhibitor of activation of nuclear transcription factor NF-kappa B. Proc Natl Acad Sci U S A. 1996 Aug 2;93(17):9090-5.

[2] Liao HF, Chen YY, Liu JJ, Hsu ML, Shieh HJ, Liao HJ, Shieh CJ, Shiao MS, Chen YJ. Inhibitory effect of caffeic acid phenethyl ester on angiogenesis, tumor invasion, and metastasis. J Agric Food Chem. 2003 Dec 31;51(27):7907-12.

Background

Caffeic acid phenethyl ester (CAPE) is an inhibitor of NF-κB activation [1].

CAPE derives from honeybee propolis and has several biological activities. It is found to be an inhibitor of NF-κB activation. In U937 cells, CAPE inhibits the TNF-dependent activation of NF-κB dose-dependently. The maximum effect occurs at dose of 25μg/ml. CAPE also inhibits NF-κB activation induced by phorbol ester, ceramide, okadaic acid and hydrogen peroxide. It is reported that CAPE prevents NF-κB from binding to DNA without affecting other transcription factors. CAPE also has angiogenesis efficacy. It shows inhibition effect on the formation of capillary-like tubes. Besides, it reduces the production of VEGF both in CT26 cell culture and in animal model. In addition, CAPE effectively prevents CT26 cells from secreting MMP-2 and MMP-9 [1, 2].

References:
[1] Natarajan K, Singh S, Burke TR Jr, Grunberger D, Aggarwal BB. Caffeic acid phenethyl ester is a potent and specific inhibitor of activation of nuclear transcription factor NF-kappa B. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9090-5.
[2] Liao HF, Chen YY, Liu JJ, Hsu ML, Shieh HJ, Liao HJ, Shieh CJ, Shiao MS, Chen YJ. Inhibitory effect of caffeic acid phenethyl ester on angiogenesis, tumor invasion, and metastasis. J Agric Food Chem. 2003 Dec 31;51(27):7907-12.

Chemical Properties

Cas No. 104594-70-9 SDF
Synonyms CAPE, 2Phenylethyl Caffeate, βPhenylethyl Caffeate
Chemical Name 2-phenylethyl (E)-3-(3,4-dihydroxyphenyl)prop-2-enoate
Canonical SMILES C1=CC=C(C=C1)CCOC(=O)C=CC2=CC(=C(C=C2)O)O
Formula C17H16O4 M.Wt 284.31
Solubility ≥ 28.4mg/mL in DMSO Storage Store at -20° C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 3.5173 mL 17.5864 mL 35.1729 mL
5 mM 0.7035 mL 3.5173 mL 7.0346 mL
10 mM 0.3517 mL 1.7586 mL 3.5173 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 17 reference(s) in Google Scholar.)

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