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Calcimycin hemimagnesium

Catalog No.GC60668

Calcimycin (A-23187) hemimagnesium is an antibiotic and a unique divalent cation ionophore (like calcium and magnesium).

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Calcimycin hemimagnesium Chemical Structure

Cas No.: 72124-77-7

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1mg
$241.00
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5mg
$667.00
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Sample solution is provided at 25 µL, 10mM.

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Background

Calcimycin (A-23187) hemimagnesium is an antibiotic and a unique divalent cation ionophore (like calcium and magnesium). Calcimycin hemimagnesium induces Ca2+-dependent cell death by increasing intracellular calcium concentration. Calcimycin hemimagnesium inhibits the growth of Gram-positive bacteria and some fungi. Calcimycin hemimagnesium also inhibits the activity of ATPase and uncouples oxidative phosphorylation (OXPHOS) of mammalian cells. Calcimycin hemimagnesium induces apoptosis[1][2][3][4].

Calcimycin (A-23187) hemimagnesium mediates mycobacterial killing by inducing intracellular calcium-regulated autophagy in a P2RX7 dependent manner[4].

Calcimycin (A-23187) hemimagnesium (2.5 or 7.5 nM; intrapleurally) induces protein leakage[5]. Animal Model: Mice (ICR, 25-30 g)[5]

[1]. Wu Q, et al. Characterization of the biosynthesis gene cluster for the pyrrole polyether antibiotic calcimycin(A23187) in Streptomyces chartreusis NRRL 3882. Antimicrob Agents Chemother. 2011 Mar;55(3):974-82. [2]. Elliott JI, et al. IKCa1 activity is required for cell shrinkage, phosphatidylserine translocation and death in Tlymphocyte apoptosis. EMBO Rep. 2003 Feb;4(2):189-94. [3]. Engedal N, et al. Modulation of intracellular calcium homeostasis blocks autophagosome formation. Autophagy. 2013 Oct;9(10):1475-90. [4]. Mawatwal S, et al. Calcimycin mediates mycobacterial killing by inducing intracellular calcium-regulated autophagy in a P2RX7 dependent manner. Biochim Biophys Acta Gen Subj. 2017 Dec;1861(12):3190-3200. [5]. Wang JP, et al. Effect of norathyriol, isolated from Tripterospermum lanceolatum, on A23187-induced pleurisy and analgesia in mice. Naunyn Schmiedebergs Arch Pharmacol. 1994 Jul;350(1):90-5.

Chemical Properties

Cas No. 72124-77-7 SDF
Canonical SMILES CNC1=CC=C2OC(C[C@@H]3[C@@H](CC[C@@]4(O3)[C@@H](C[C@H]([C@H](O4)[C@@H](C(C5=CC=CN5)=O)C)C)C)C)=NC2=C1C([O-][Mg+2][O-]C(C6=C7N=C(C[C@H]8O[C@]9(O[C@H]([C@H](C)C(C%10=CC=CN%10)=O)[C@H](C)C[C@H]9C)CC[C@H]8C)OC7=CC=C6NC)=O)=O
Formula C58H72MgN6O12 M.Wt 1069.53
Solubility Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 0.935 mL 4.675 mL 9.3499 mL
5 mM 0.187 mL 0.935 mL 1.87 mL
10 mM 0.0935 mL 0.4675 mL 0.935 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 23 reference(s) in Google Scholar.)

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