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O4I2

Catalog No.GC17042

Oct3/4 inducer

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O4I2 Chemical Structure

Cas No.: 165682-93-9

Size Price Stock Qty
10mg
$53.00
In stock
50mg
$158.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Target: Oct3; Oct4

IC50: N/A

O4I2, a potent Oct3/4 inducer, is suitable for iPSC generation [1]. The octamer-binding transcription factor 4 (Oct3/4), a member of the POU family of transcription factors, is expressed specifically in pluripotent cells such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs). Oct3/4 plays a critical role in regulation of the cellular pluripotency core network. The loss-of-function mutation or suppression of Oct3/4 in embryonic stem cells (ESCs) leads to differentiation of ESCs into trophoblast and loss of pluripotency, whereas gain-of-function or forced overexpression Oct3/4, can reprogram somatic cells back into pluripotent stem cells (iPSCs) [1].

In vitro: O4I2 (5, 10, and 20 μM) dose-dependently activated Oct3/4 expression in HEK293 cells, embryonal NCCIT cells, and also in Oct-deficient HeLa cells [1]. In addition, O4I2 (10, and 20 μM) activated Oct3/4 on the transcriptional and translational level in human neonatal foreskin fibroblasts (HFFs), and promoted pluripotency-associated genes (Nanog and Lin28) expression [1].

In vivo: N/A

Reference:
1.  Cheng X, Yoshida H, Raoofi D, Saleh S, Alborzinia H, Wenke F, et al. Ethyl 2-((4-Chlorophenyl)amino)thiazole-4-carboxylate and Derivatives Are Potent Inducers of Oct3/4. J Med Chem. 2015;58(15):5742-50.

Chemical Properties

Cas No. 165682-93-9 SDF
Chemical Name ethyl 2-((4-chlorophenyl)amino)thiazole-4-carboxylate
Canonical SMILES CCOC(C1=CSC(NC2=CC=C(Cl)C=C2)=N1)=O
Formula C12H11ClN2O2S M.Wt 282.75
Solubility ≥ 28.3mg/mL in DMSO Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 3.5367 mL 17.6835 mL 35.3669 mL
5 mM 0.7073 mL 3.5367 mL 7.0734 mL
10 mM 0.3537 mL 1.7683 mL 3.5367 mL
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 3 reference(s) in Google Scholar.)

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