Biotin-tyramide (Synonyms: Biotin-Phenol,Biotin Phenol) |
Catalog No.GC13376 |
Biotin-tyramide is a biotin derivative used for tyramide signal amplification (TSA), as a reagent to amplify both immunohistochemical signals and in situ hybridization protocols.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 41994-02-9
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1]: | |
Tissue |
Brain tissue from Long–Evans rats |
Preparation Method |
Brain tissue from six animals was processed in one run to fluorescently co-label ARs and NeuN, with and without Biotin-tyramide, as well as ARs and GFAP, with and without Biotin-tyramide. |
Reaction Conditions |
Biotin-tyramide for 30min |
Applications |
Biotin-tyramide signal amplification (TSA) dramatically increases AR immunoreactivity in the rat brain, including critical regions of the PFC such as the medial PFC (mPFC) and orbitofrontal cortex (OFC). Biotin-tyramide signal amplification is useful for AR detection with both chromogenic and immunofluorescent immunohistochemistry. |
References: [1]. Low KL, Ma C, et,al. Tyramide Signal Amplification Permits Immunohistochemical Analyses of Androgen Receptors in the Rat Prefrontal Cortex. J Histochem Cytochem. 2017 May;65(5):295-308. doi: 10.1369/0022155417694870. Epub 2017 Mar 1. PMID: 28438093; PMCID: PMC5407533. |
Biotin-tyramide is used for tyramide signal amplification (TSA) which is a powerful, patented technology that significantly enhances both chromogenic and fluorescent signals. TSA is easily integrated into standard nonradioactive in situ hybridization (ISH) or IHC protocols.
TSA is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to catalyze the deposition of a fluorophore-labeled tyramide amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins.
Biotin-tyramide use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.
Cas No. | 41994-02-9 | SDF | |
Synonyms | Biotin-Phenol,Biotin Phenol | ||
Chemical Name | N-(4-hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide | ||
Canonical SMILES | O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(NCCC3=CC=C(O)C=C3)=O | ||
Formula | C18H25N3O3S | M.Wt | 363.47 |
Solubility | ≥ 72.6mg/mL in DMSO, ≥ 8.18 mg/mL in EtOH with ultrasonic | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.7513 mL | 13.7563 mL | 27.5126 mL |
5 mM | 0.5503 mL | 2.7513 mL | 5.5025 mL |
10 mM | 0.2751 mL | 1.3756 mL | 2.7513 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
(Based on Reviews and 3 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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