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Ac-VDVAD-AFC (Synonyms: N-Acetyl-Val-Asp-Val-Ala-Asp-7-amino-4-Trifluoromethylcoumarin Caspase-2 Substrate (Fluorogenic))

Catalog No.GC13400

Ac-VDVAD-AFC is a caspase-specific fluorescent substrate. Ac-VDVAD-AFC can measure caspase-3-like activity and caspase-2 activity and can be used for the research of tumor and cancer.

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Ac-VDVAD-AFC Chemical Structure

Cas No.: 210344-94-8

Size Price Stock Qty
1mg
$106.00
In stock
5mg
$288.00
In stock
10mg
$524.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Description Chemical Properties Product Documents Related Products

Ac-VDVAD-AFC is a fluorogenic substrate whose amino acids VDVAD have been shown to be a preferred cleavage site for caspase-2.1,2 Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm. Increases in Ac-VDVAD-AFC cleavage have been reported to correlate with nitric oxide-induced activation of caspase-2 and subsequent apoptosis in human neuroblastoma lines.1

Product Description References

1. Moriya, R., Uehara, T., and Nomura, Y. Mechanism of nitric oxide-induced apoptosis in human neuroblastoma SH-SY5Y cells. FEBS Lett. 484(3), 253-260 (2000).

2. Talanian, R.V., Quinlan, C., Trautz, S., et al. Substrate specificities of caspase family proteases. The Journal of Biological Chemisty 272(15), 9677-9682 (1997).

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