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Chrysin Catalog No.GN10028

Anti-inflammatory and antitumor reagent

Size Price Stock Qty
10mM (in 1mL DMSO)
$35.00
In stock
20mg
$75.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & SDS

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Protocol

Cell experiment:

The proliferation assays are conducted using a cell proliferation enzyme-linked immunosorbent assay (ELISA) 5-bromo-2'-deoxyuridine (BrdU) kit. Briefly, ES2 and OV90 cells are seeded in a 96-well plate, and then treated with Chrysin (0, 5, 10, 20, 50, and 100 µM) with or without inhibitors (20 μM LY294002, PI3K/AKT; 10 μM U0126, ERK1/2; 10 μM SP600125, JNK; and 20 μM SB203580, p38) in a final volume of 100 μL/well. Aftera48-h incubation, 10 μM BrdU is added to the cell culture, followed by an additional 2-h incubation at 37°C. After labeling the cells with BrdU, they are fixed and then incubated with the anti-BrdU-peroxidase (POD) working solution for 90 min. The anti-BrdU-POD binds to the BrdU incorporated into newly synthesized cellular DNA and these immune complexes are detected by analyzing their reaction with the 3,3',5,5'-tetramethylbenzidine (TMB) substrate. The absorbance values of the reaction product are measured at 370 and 492 nm using an ELISA reader[1].

References:

[1]. Lim W, et al. Chrysin Attenuates Progression of Ovarian Cancer Cells by Regulating Signaling Cascades and Mitochondrial Dysfunction. J Cell Physiol. 2017 Aug 17.

Chemical Properties

Cas No. 480-40-0 SDF
Chemical Name 5,7-dihydroxy-2-phenylchromen-4-one
Canonical SMILES C1=CC=C(C=C1)C2=CC(=O)C3=C(C=C(C=C3O2)O)O
Formula C15H10O4 M.Wt 254.24
Solubility ≥12.4mg/mL in DMSO Storage
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
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Background

Chrysin is one of the most well known estrogen blockers.

Chrysin is mainly found in passion flowers, honey, and propolis acts as a potential therapeutic and preventive agent to inhibit proliferation and invasion of various human cancer cells. Although Chrysin has anti-carcinogenic effects in several cancers, little is known about its functional roles in ovarian cancer which shows poor prognosis and chemoresistance to traditional therapeutic agents. Chrysin inhibits ovarian cancer cell proliferation and induced cell death by increasing reactive oxygen species (ROS) production and cytoplasmic Ca2+ levels as well as inducing loss of mitochondrial membrane potential (MMP). Chrysin activates MAPK and PI3K/AKT pathways in ES2 and OV90 cells in concentration-response experiments. Chrysin suppresses tumor growth byregulating canonical Wnt and nuclear factor NF-κB signaling cascades cancer cells. Chrysin stimulates the phosphorylation of AKT and P70S6K proteins in both ES2 and OV90 cells compared tothe untreated control cells. In addition, Chrysin activates the phospho-ERK1/2, p38,and JNK proteins as members of the MAPK pathway in the ovarian cancer cells[1].

References:
[1]. Lim W, et al. Chrysin Attenuates Progression of Ovarian Cancer Cells by Regulating Signaling Cascades and Mitochondrial Dysfunction. J Cell Physiol. 2017 Aug 17.