Citrullinated Histone H3 (R2 + R8 + R17) (2-22)-biotin Peptide (Synonyms: Biotin-A-Cit-TKQTA-Cit-KSTGGKAP-Cit-KQLA, Biotin-AXTKQTAXKSTGGKAPXKQLA (X=Citrulline), Citrullinated Histone H3.1-biotin) |
| Catalog No.GC52363 |
A biotinylated and citrullinated histone H3 peptide
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Citrullinated histone H3 (R2 + R8 + R17) (2-22)-biotin peptide is a biotinylated version of tri-citrullinated histone H3, a nuclear protein that interacts with linker DNA regions between nucleosomes to provide nucleosome core structure stabilization.1 Histone H3 is subject to citrullination by protein-arginine deiminase 4 (PAD4) at arginine 2 (R2), R8, and R17 during neutrophil extracellular trap formation (NETosis).2 Citrullination of histone H3 at these residues reduces the positive charge of histone H3 and leads to decondensed chromatin, which is expelled into the extracellular space when the neutrophil membrane ruptures.3,2 Increased serum levels of histone H3 citrullinated at R2, R8, and R17 have been found in patients with asthma and in septic shock patients where they are positively associated with a sequential organ failure assessment (SOFA) score and poor survival.4,5 Sputum levels of histone H3 citrullinated at R2, R8, and R17 are increased in patients with rheumatoid arthritis, as well as in individuals identified as at risk for developing rheumatoid arthritis based on their seropositivity for IgA anti-citrullinated protein antibodies (ACPAs).6 's Citrullinated Histone H3 (R2 + R8 + R17) (2-22)-biotin Peptide is intended for use in the detection of autoantibodies against histone H3 citrullinated at R2, R8, and R17.
1.Roque, A., Ponte, I., and Suau, P.Interplay between histone H1 structure and functionBiochim. Biophys. Acta1859(3)444-454(2016) 2.Wang, Y., Li, M., Stadler, S., et al.Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formationJ. Cell Biol.184(2)205-213(2009) 3.Li, M., Lin, C., Leso, A., et al.Quantification of citrullinated histone H3 bound DNA for detection of neutrophil extracellular trapsCancers (Basel)12(11)3424(2020) 4.Kuczia, P., Zuk, J., Iwaniec, T., et al.Citrullinated histone H3, a marker of extracellular trap formation, is increased in blood of stable asthma patientsClin. Transl. Allergy1031(2020) 5.Tian, Y., Russo, R.M., Li, Y., et al.Serum citrullinated histone H3 concentrations differentiate patients with septic verses non-septic shock and correlate with disease severityInfection49(1)83-93(2021) 6.Okamoto, Y., Devoe, S., Seto, N., et al.Association of sputum neutrophil extracellular trap subsets with IgA anti-citrullinated protein antibodies in subjects at risk for rheumatoid arthritisArthritis Rheumatol.74(1)38-48(2022)
| Cas No. | SDF | ||
| Synonyms | Biotin-A-Cit-TKQTA-Cit-KSTGGKAP-Cit-KQLA, Biotin-AXTKQTAXKSTGGKAPXKQLA (X=Citrulline), Citrullinated Histone H3.1-biotin | ||
| Formula | M.Wt | 0 | |
| Solubility | Storage | -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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