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Crosstide (trifluoroacetate salt) (Synonyms: Gly-Arg-Pro-Arg-Thr-Ser-Ser-Phe-Ala-Glu-Gly-OH, GRPRTSSFAEG-OH)

Catalog No.GC52387

A peptide substrate for Akt

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Crosstide (trifluoroacetate salt) Chemical Structure

Size Price Stock Qty
1 mg
$270.00
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5 mg
$675.00
In stock
10 mg
$1,078.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Crosstide is a synthetic peptide substrate for Akt.1 It corresponds to the amino acid sequence of glycogen synthase kinase 3 (GSK3) that surrounds the serine residue phosphorylated by p90 ribosomal S6 kinase 1 (RSK1) or p70 ribosomal S6 kinase (p70S6K). Crosstide has been used in the study of Akt activity.1,2 It is also a substrate for serum/glucocorticoid-regulated kinase family member 3 (SGK3).3

1.Cross, D.A.E., Slessi, D.R., Cohen, P., et al.Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase BNature378(6559)785-789(1995) 2.Tanti, J.-F., Grillo, S., Gremeaux, T., et al.Potential role of protein kinase B in glucose transporter 4 translocation in adipocytesEndocrinology138(5)2005-2010(1997) 3.Dai, F., Yu, L., He, H., et al.Human serum and glucocorticoid-inducible kinase-like kinase (SGKL) phosphorylates glycogen syntheses kinase 3 beta (GSK-3β) at serine-9 through direct interactionBiochem. Biophys. Res. Commun.293(4)1191-1196(2002)

Chemical Properties

Cas No. SDF Download SDF
Synonyms Gly-Arg-Pro-Arg-Thr-Ser-Ser-Phe-Ala-Glu-Gly-OH, GRPRTSSFAEG-OH
Canonical SMILES N=C(N)NCCC[C@H](NC(CN)=O)C(N1[C@@H](CCC1)C(N[C@@H](CCCNC(N)=N)C(N[C@@H]([C@H](O)C)C(N[C@@H](CO)C(N[C@@H](CO)C(N[C@H](C(N[C@@H](C)C(N[C@@H](CCC(O)=O)C(NCC(O)=O)=O)=O)=O)CC2=CC=CC=C2)=O)=O)=O)=O)=O)=O.OC(C(F)(F)F)=O
Formula C48H77N17O17 ? XCF3COOH M.Wt 1164.2
Solubility DMF: insol,DMSO: insol,Ethanol: insol,PBS (pH 7.2): 1 mg/ml Storage -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 0.859 mL 4.2948 mL 8.5896 mL
5 mM 0.1718 mL 0.859 mL 1.7179 mL
10 mM 0.0859 mL 0.4295 mL 0.859 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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