Cy5-UTP, 10mM Sodium Solution
Sample solution is provided at 25 µL, 10mM.
GlpBio Products Cited In Reputable Papers
Cy5-UTP (Cyanine 5-aminoallyluridine-50-triphosphate) acts as a substrate for T7 RNA polymerase which could replace UTP with cyanine5-UTP. It is used to label RNA probes through in vitro transcription. The assembled mRNA will emit orange fluorescence and this incorporation allows RNA and mRNA detection independent of protein translation. Moreover, Cy5-labeled mRNA is visible under ultraviolet light without further staining after gel electrophoresis. Cy5-labeled RNA probes are used in a majority of molecular biology applications, such as mutagenesis, in situ hybridization, photo-cross linking studies and gene expression microarrays, etc.
.Custer, T C., et al. In vitro labeling strategies for in cellulo fluorescence microscopy of single ribonucleoprotein machines. PROTEIN SCIENCE 2017 vol. 26:1363—1379.
|Solubility||N/A||Storage||Store at -20°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
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Analysis of oligonucleotide microarrays by 3' end labeling using fluorescent nucleotides and terminal transferase
Biotechniques2006 Jul;41(1):53-6.PMID: 16869513DOI: 10.2144/000112182
A simple enzymatic labeling procedure is described to determine spot quality in oligonucleotide microarrays. By using fluorescently labeled dideoxynucleotides or ribonucleotides as substrate for terminal deoxynucleotidyl transferase (TdT), a single fluorophore can be covalently attached at the 3' end of each oligonucleotide probe molecule in the spot. Fluorescein-12-ddUTP CyTM3-ddUTP Cy5-UTP, and Cy3-UTP were compared as TdT substrates for 3' end labeling an array of 1273 hexamer probes. Cy5-UTP was found to show minimal bias toward probe base composition and is therefore well suited for quantitative analysis of microarray spots where the oligonucleotide probes are coupled via a 5' end linkage to the solid phase.
Average Rating: 5(Based on Reviews and 30 reference(s) in Google Scholar.)
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