Cy5-UTP, 10mM Sodium Solution |
Catalog No.GB20007 |
Cy5-UTP is a Cyanine5-labeled UTP analog that can replace UTP for RNA labeling in in vitro transcription reactions.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Cyanine5-UTP (Cy5-UTP) can replace UTP as the substrate of T7 RNA polymerase, and generate orange fluorescently labeled mRNA through in vitro transcription, excitation/emission (nm): 650/665nm[1]. Probes generated using Cy5-UTP as a substrate are suitable for multicolor fluorescence analysis, especially dual-color expression arrays combined with Cy5-UTP [2].
References:
[1]. Custer TC, et al. In vitro labeling strategies for in cellulo fluorescence microscopy of single ribonucleoprotein machines. Protein Sci. 2017 Jul;26(7):1363-1379.
[2]. Guerra (2006) Analysis of oligonucleotide microarrays by 3' end labeling using fluorescent nucleotides and terminal transferase. Biotechniques 41 (1):53.
Cas No. | SDF | ||
Chemical Name | O=S(C1=CC2=C(N(CCCCCC(NC/C=C/C3=CN([C@H]4[C@H](O)[C@H](O)[C@@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O4)C(NC3=O)=O)=O)/C(C2(C)C)=C/C=C/C=C/C(C5(C)C)=[N+](CC)C6=C5C=C(S([O-])(=O)=O)C=C6)C=C1)(O)=O | ||
Formula | C45H58N5O22P3S2 | M.Wt | 1178.01 |
Solubility | A solution in water | Storage | Store at -20°C,protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.8489 mL | 4.2444 mL | 8.4889 mL |
5 mM | 0.1698 mL | 0.8489 mL | 1.6978 mL |
10 mM | 0.0849 mL | 0.4244 mL | 0.8489 mL |
Average Rating: 5
(Based on Reviews and 30 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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