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Cy5-UTP, 10mM Sodium Solution

Catalog No.GB20007

Cy5-UTP, 10mM Sodium Solution Chemical Structure

Cy5-UTP (Cyanine 5-aminoallyluridine-50-triphosphate) acts as a substrate for T7 RNA polymerase which could replace UTP with cyanine5-UTP.

Size Price Stock Qty
25μL
$180.00
In stock
1mL Please Inquire Please Inquire
10mL Please Inquire Please Inquire
100μL
$480.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Background

Cy5-UTP (Cyanine 5-aminoallyluridine-50-triphosphate) acts as a substrate for T7 RNA polymerase which could replace UTP with cyanine5-UTP. It is used to label RNA probes through in vitro transcription. The assembled mRNA will emit orange fluorescence and this incorporation allows RNA and mRNA detection independent of protein translation. Moreover, Cy5-labeled mRNA is visible under ultraviolet light without further staining after gel electrophoresis. Cy5-labeled RNA probes are used in a majority of molecular biology applications, such as mutagenesis, in situ hybridization, photo-cross linking studies and gene expression microarrays, etc.[1]

References:
[1].Custer, T C., et al. In vitro labeling strategies for in cellulo fluorescence microscopy of single ribonucleoprotein machines. PROTEIN SCIENCE 2017 vol. 26:1363—1379.

Chemical Properties

Cas No. N/A SDF N/A
Synonyms N/A
Chemical Name N/A
Canonical SMILES N/A
Formula C45H58N5O22P3S2(freeacid) M.Wt 1178.01(freeacid)
Solubility N/A Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Research Update

Analysis of oligonucleotide microarrays by 3' end labeling using fluorescent nucleotides and terminal transferase

Biotechniques2006 Jul;41(1):53-6.PMID: 16869513DOI: 10.2144/000112182

A simple enzymatic labeling procedure is described to determine spot quality in oligonucleotide microarrays. By using fluorescently labeled dideoxynucleotides or ribonucleotides as substrate for terminal deoxynucleotidyl transferase (TdT), a single fluorophore can be covalently attached at the 3' end of each oligonucleotide probe molecule in the spot. Fluorescein-12-ddUTP CyTM3-ddUTP Cy5-UTP, and Cy3-UTP were compared as TdT substrates for 3' end labeling an array of 1273 hexamer probes. Cy5-UTP was found to show minimal bias toward probe base composition and is therefore well suited for quantitative analysis of microarray spots where the oligonucleotide probes are coupled via a 5' end linkage to the solid phase.

Keywords:

Cy5 UTP

CY5-UTP

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