Home>>Modified Nucleotides>>Cy5-UTP, 10mM Sodium Solution

Cy5-UTP, 10mM Sodium Solution

Catalog No.GB20007

Cy5-UTP is a Cyanine5-labeled UTP analog that can replace UTP for RNA labeling in in vitro transcription reactions.

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Cy5-UTP, 10mM Sodium Solution Chemical Structure

Size Price Stock Qty
25μL
$180.00
In stock
100μL
$480.00
In stock
500μL
$1,500.00
In stock
1mL
$2,400.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Cyanine5-UTP (Cy5-UTP) can replace UTP as the substrate of T7 RNA polymerase, and generate orange fluorescently labeled mRNA through in vitro transcription, excitation/emission (nm): 650/665nm[1]. Probes generated using Cy5-UTP as a substrate are suitable for multicolor fluorescence analysis, especially dual-color expression arrays combined with Cy5-UTP [2].

References:
[1]. Custer TC, et al. In vitro labeling strategies for in cellulo fluorescence microscopy of single ribonucleoprotein machines. Protein Sci. 2017 Jul;26(7):1363-1379.
[2]. Guerra (2006) Analysis of oligonucleotide microarrays by 3' end labeling using fluorescent nucleotides and terminal transferase. Biotechniques 41 (1):53.

Chemical Properties

Cas No. SDF
Chemical Name O=S(C1=CC2=C(N(CCCCCC(NC/C=C/C3=CN([C@H]4[C@H](O)[C@H](O)[C@@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O4)C(NC3=O)=O)=O)/C(C2(C)C)=C/C=C/C=C/C(C5(C)C)=[N+](CC)C6=C5C=C(S([O-])(=O)=O)C=C6)C=C1)(O)=O
Formula C45H58N5O22P3S2 M.Wt 1178.01
Solubility A solution in water Storage Store at -20°C,protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 0.8489 mL 4.2444 mL 8.4889 mL
5 mM 0.1698 mL 0.8489 mL 1.6978 mL
10 mM 0.0849 mL 0.4244 mL 0.8489 mL
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