3X FLAG Peptide (Synonyms: H-Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH) |
Katalog-Nr.GP10149 |
Synthetisches Peptid-Tag
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 402750-12-3
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Protocol for Protein expression and purification by 3×FLAG peptide [1]: |
This protocol only provides a guideline, and should be modified according to your specific needs. |
Protocol for Co-IP and co-IP–MS analyses using 3×FLAG peptide [2]: |
This protocol only provides a guideline, and should be modified according to your specific needs. |
References: [1]. Gao XK, Rao XS, et al. Phase separation of insulin receptor substrate 1 drives the formation of insulin/IGF-1 signalosomes. Cell Discov. 2022 Jun 28;8(1):60. doi: 10.1038/s41421-022-00426-x. PMID: 35764611; PMCID: PMC9240053. [2]. Cong M, Wang Y, et al. MTSS1 suppresses mammary tumor-initiating cells by enhancing RBCK1-mediated p65 ubiquitination. Nat Cancer. 2020 Feb;1(2):222-234. doi: 10.1038/s43018-019-0021-y. Epub 2020 Jan 20. PMID: 35122005. |
Das FLAG-Tag-System verwendet ein kurzes, hydrophiles 8-Aminosäure-Peptid, das mit dem Protein von Interesse fusioniert wird. Das FLAG-Peptid bindet an den Antikörper M1. Ob die Bindung calciumabhängig oder -unabhängig ist, bleibt umstritten. Ein Nachteil des Systems besteht darin, dass die Monoklonalantikörper-Reinigungsmatrix nicht so stabil ist wie andere. Im Allgemeinen können kleine Tags mit spezifischen monoklonalen Antikörpern nachgewiesen werden.
Um die Erkennung des FLAG-Tags zu verbessern, wurde das 3x-FLAG-System entwickelt. Dieses dreifache Flag-Epitop ist hydrophil, 22 Aminosäuren lang und kann bis zu 10 fmol exprimiertes Fusionsprotein erkennen. Das mit einem FLAG-Tag versehene Malto-Dextrin-bindende Protein von Pyrococcus furiosus wurde kristallisiert und die Qualität der Kristalle war sehr ähnlich wie bei Kristallen des unmarkierten Proteins.
Schließlich kann das FLAG-Tag durch Behandlung mit Enterokinase entfernt werden, die spezifisch für die fünf C-terminalen Aminosäuren der Peptidsequenz ist.
Referenzen:
1. Hopp TP, Prickett KS, Price VL, Libby RT, March CJ, Ceretti DP, Urdal DL, Conlon PJ (1988) Eine kurze Polypeptid-Markersequenz zur Identifikation und Reinigung von rekombinanten Proteinen. Bio/Technology 6:1204每1210.
2. Hopp TP, Gallis B, Prikett KS (1996) Metallbindende Eigenschaften eines calciumabhängigen monoklonalen Antikörpers. Mol Immunol 33:601每608.
3. Einhauer A., Jungbauer A (2000) Kinetik und thermodynamische Eigenschaften des monoklonalen Antikörpers M1 gegen das FLAG-Peptid. 20th International symposium on the separation of proteins, peptides and polynucleotides (ISPPP). Lublijana Slowenien vom 5.-8.November 2000.
4. Bucher MH., Evdokimov AG., Waugh DS.(2002) Unterschiedliche Auswirkungen kurzer Affinitätsmarkierungen auf die Kristallisation des Pyrococcus furiosus Maltodextrin-bindenden Proteins.Biol Cryst58:392每397.
5.Maroux S., Baratti J., Desnuelle P.(1971) Reinigung und Spezifität der Schweine-Enterokinase.J Biol Chem246:5031每5039.
Cas No. | 402750-12-3 | SDF | |
Überlieferungen | H-Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH | ||
Chemical Name | 3X FLAG Peptide | ||
Canonical SMILES | CCC(C)C(C(=O)NC(CC(=O)O)C(=O)NC(CC1=CC=C(C=C1)O)C(=O)NC(CCCCN)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CCCCN)C(=O)O)NC(=O)C(CC(=O)O)NC(=O)C(CC2=CNC=N2)NC(=O)C(CC(=O)O)NC(=O)C(CCCCN)NC(=O)C(CC3=CC=C(C=C3)O)NC(=O)C(CC(=O)O)NC( | ||
Formula | C120H169N31O49S | M.Wt | 2861.87 |
Löslichkeit | ≥143.1mg/mL in DMSO, <14.35mg/mL in EtOH, ≥143.4mg/mL in Water with gentle warming | Storage | Desiccate at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.3494 mL | 1.7471 mL | 3.4942 mL |
5 mM | 0.0699 mL | 0.3494 mL | 0.6988 mL |
10 mM | 0.0349 mL | 0.1747 mL | 0.3494 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
(Based on Reviews and 29 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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