Artesunate (Synonyms: Artesunic Acid, NSC 712571, WR 256283) |
Katalog-Nr.GC10889 |
Ableitung des Naturprodukts Artemisinin
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 88495-63-0
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Kinase experiment: | After treatment with Artesunate for 24 h, cells are harvested and lysed in 1×cell lysis buffer. Total proteins of 15 to 25 μg are separated by SDS-PAGE and transferred to polyvinylidenedifluoride (PVDF) membranes. Membranes are blocked with 5% non-fat milk for 1 to 2 h at room temperature and then probed with primary antibodies and incubated at 4°C overnight. After extensive washing with TBS-T, membranes are incubated with appropriate HRP-conjugated secondary antibody for 1 h at room temperature, and then are detected by Western ECL-enhanced luminol reagent [3]. |
Cell experiment: | A2780 and HO8910 cells are cultured in RPMI 1640, Normal human fibroblasts (NHF) in DMEM, and FTE-187 in M199, supplemented with 10% fetal bovine serum, 100 units/mL penicillin, and 100 mg/mL streptomycin. All the cells are incubated in a humidified atmosphere of 95% air and 5% CO2. Artesunate is applied to the cultured cells at the concentration of 0, 5, 10, 25, or 50 µg/mL for various periods. The reactive oxygen species (ROS) production following Artesunate treatment is determined. Briefly, cells are loaded with 5 μM of CM-H2DCFDA and incubated at 37°C for 20 min after treatment with Artesunate. Cells are resuspended using preserving fluid and analyzed with a FACSCanto II. The peak excitation wavelength for oxidized CM-H2DCFDA is 490 nm and emission is 530 nm[3]. |
Animal experiment: | Four to six weeks old female athymic nude mice (BALB/c, nu/nu) are used. A2780 and HO8910 cells are harvested and resuspended in 0.1 ml of PBS, 5×106 cells/0.2 mL are injected subcutaneously into the left inguinal area of the mice. Two weeks later, mice bearing tumors (~70 mm3 for A2780 and HO8910) are randomly divided into 4 groups. Artesunate is administered daily via i.p. injection at doses of 50 mg/kg alone for 16 days. The tumor growth is monitored every other day. Tumor volume is determined by the formula 1/2a×b2 where a is the long diameter (mm) and b is the short diameter (mm)[3]. |
References: [1]. Ilamathi M, et al. Artesunate as an Anti-Cancer Agent Targets Stat-3 and Favorably Suppresses Hepatocellular Carcinoma. Curr Top Med Chem. 2016;16(22):2453-63. |
Artesunat ist ein Hemmstoff sowohl von STAT-3 als auch von exportiertem Protein 1 (EXP1).
Artesunat ist ein Inhibitor sowohl von STAT-3[1] als auch von exportiertem Protein 1 (EXP1)[2]. Eine Behandlung mit Artesunat für 24 Stunden führt in beiden Zelllinien dosisabhängig zu einer signifikanten Erhöhung der reaktiven Sauerstoffspezies (ROS). Darüber hinaus zeigt die Western-Blot-Analyse, dass die γ-H2AX-Spiegel signifikant erhöht sind, wenn Krebszellen im höheren Dosisbereich für 24 Stunden mit Artesunat behandelt werden. Artesunat zeigt auch eine zeitabhängige Wirkung auf den RAD51-Spiegel in A2780- und HO8910-Zellen. Bei zwei Arten von nicht-malignen Zellen, normalen menschlichen Fibroblasten und immortalisierten epithelialen Zellen FTE-187, wird der RAD51-Spiegel durch Artesunat nicht verändert. In A2780-Zellen wird der RAD51-mRNA-Spiegel tatsächlich durch Zugabe von Artesunat dosisabhängig verringert. Entsprechend wird die Promotoraktivität von RAD51 signifikant durch Artesunat gehemmt. Im Gegensatz dazu wird der RAD51-mRNA-Spiegel in H8910-Zellen nicht durch Artesunate beeinflusst[3].
Das Tumorwachstum wird signifikant reduziert in der Gruppe, die eine kombinierte Behandlung mit Artesunat und Cisplatin erhält (P<0.01). Im Vergleich dazu hat Artesunat allein keinen signifikanten Effekt auf das Wachstum von Tumor-Xenografts für beide Zelllinien[3].
References:
[1]. Ilamathi M, et al. Artesunate as an Anti-Cancer Agent Targets Stat-3 and Favorably Suppresses Hepatocellular Carcinoma. Curr Top Med Chem. 2016;16(22):2453-63.
[2]. Lisewski AM, et al. Supergenomic network compression and the discovery of EXP1 as a glutathione transferase inhibited by artesunate. Cell. 2014 Aug 14;158(4):916-928.
[3]. Wang B, et al. Artesunate sensitizes ovarian cancer cells to cisplatin by downregulating RAD51. Cancer Biol Ther. 2015;16(10):1548-56.
Cas No. | 88495-63-0 | SDF | |
Überlieferungen | Artesunic Acid, NSC 712571, WR 256283 | ||
Canonical SMILES | O=C(CCC(O)=O)O[C@H]1O[C@@H]2O[C@@]3(C)OO[C@]42[C@@H](CC3)[C@H](C)CC[C@H]4[C@H]1C | ||
Formula | C19H28O8 | M.Wt | 384.42 |
Löslichkeit | ≥ 16.3mg/mL in DMSO | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.6013 mL | 13.0066 mL | 26.0132 mL |
5 mM | 0.5203 mL | 2.6013 mL | 5.2026 mL |
10 mM | 0.2601 mL | 1.3007 mL | 2.6013 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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