Biotin-16-UTP |
| Katalog-Nr.GB20150 |
Biotin-16-UTP is a biotin-labeled UTP used for in vitro transcription to prepare RNA probes.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 280549-77-1 (free acid)
Sample solution is provided at 25 µL, 10mM.
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Biotin-16-UTP is a biotin-labeled UTP used for in vitro transcription to prepare RNA probes[1]. Biotin-16-UTP is an active substrate for RNA polymerase and can be incorporated by a variety of RNA polymerases (including SP6, T3, and T7 RNA polymerases) to form labeled RNA[2, 3]. Biotin-16-UTP-labeled RNA can be detected by reporter molecules linked to streptavidin, avidin, or anti-biotin antibodies[4, 5]. Biotin-16-UTP can be used for molecular hybridization and RNA-protein interaction studies[6].
References:
[1]McKenney K M, Connacher R P, Dunshee E B, et al. Chemi-Northern: a versatile chemiluminescent northern blot method for analysis and quantitation of RNA molecules[J]. RNA, 2024, 30(4): 448-462.
[2]Chien H, Alston C I, Dix R D. Suppressor of cytokine signaling 1 (SOCS1) and SOCS3 are stimulated within the eye during experimental murine cytomegalovirus retinitis in mice with retrovirus-induced immunosuppression[J]. Journal of Virology, 2018, 92(18): 10.1128/jvi. 00526-18.
[3]Chen M, Zhang L, Guo Y, et al. A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1‐RhoA/Rac1[J]. Journal of Cellular and Molecular Medicine, 2021, 25(13): 5988-6005.
[4]Ritchey B, Hai Q, Han J, et al. Genetic variant in 3’untranslated region of the mouse pycard gene regulates inflammasome activity[J]. Elife, 2021, 10: e68203.
[5]Bratthauer G L. The avidin–biotin complex (ABC) method and other avidin–biotin binding methods[J]. Immunocytochemical methods and protocols, 2010: 257-270.
[6]Siprashvili Z, Webster D E, Kretz M, et al. Identification of proteins binding coding and non-coding human RNAs using protein microarrays[J]. BMC genomics, 2012, 13: 1-13.
This plan only provides a guide, please modify it to meet your specific needs.
1. Solution preparation
(1) Stock solution: This product is provided in 10mM stock liquid form.
Note: After the unused stock solution is aliquoted, store it at -20°C or -80°C in the dark to avoid repeated freezing and thawing.
(2) Working solution: Dilute the stock solution with the experimental buffer to prepare a working concentration of 0.5mM.
Note: Please adjust the working solution concentration according to the actual situation and prepare it before use.
2. Preparation of RNA probe with Biotin-16-UTP[1](from the literature, for reference only)
(1) Template DNA preparation: In a 0.5mL test tube, add 1μg purified template DNA or 4μL control DNA to sterile, RNase-free diethyl pyrocarbonate (DEPC) water, and the total sample volume is 13μL.
(2) Preparation of in vitro transcription reaction system: For each sample, add 2μL 10× deoxynucleoside triphosphate mixture, 2μL 10× transcription buffer, 1μL Protector RNase inhibitor and 2μL RNA polymerase SP6 or RNA polymerase T7.
(3) Labeling reaction: Add Biotin-16-UTP (final concentration is usually 0.5mM) and incubate at 37°C for 2h to prepare RNA probe.
(4) Termination reaction: Add 2mL RNase-free-DNase I to each tube to remove template DNA and incubate at 37°C for 10min. Add 2mL 0.2M EDTA (pH 8.0) to each tube to terminate the DNase I reaction.
(5) Isolation of RNA probe: Isolate RNA probe by lithium chloride precipitation and resuspend in 25μL DEPC water.
References:
[1] Bharati B K, Swetha R K, Chatterji D. Identification and characterization of starvation induced msdgc-1 promoter involved in the c-di-GMP turnover[J]. Gene, 2013, 528(2): 99-108.
| Cas No. | 280549-77-1 (free acid) | SDF | |
| Formula | C32H52N7O19P3S (free acid) | M.Wt | 963.8 (free acid) |
| Löslichkeit | Storage | Store at -20°C or below,always avoid freeze-thaw cycles and avoid direct sunlight. | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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