Lipo3000 is a type of transfection reagent which is lipid-based in nature and used to transfect cells by transporting the complex molecules into the cell. Due to its cationic nature, it is used in a wide variety of techniques i.e. gene expression or gene delivery.

Glioblastoma (GBM) is a high-grade glioma characterized by a high rate of invasiveness and rapid growth. Despite advances in surgical and medical treatment modalities, very few patients with GBM survive beyond 15 months after diagnosis. Currently, temozolomide (TMZ) is the main first-line drug for GBM chemotherapy, but it is prone to drug resistance. Therefore, it is urgent to identify other drugs with better efficacy to treat GBM.

It is a type of reagent that is cationic, liposome-based, and used in a wide variety of in-vitro techniques in mammalian cells for transfection, and transgene expressions. The efficiency of transfection and viability of cell depends upon many factors which are given as follows:

• Media components (antibiotics & serum level)

• Liposome

• Cell density

• DNA-liposome complexing time

• DNA concentration

D-Luciferin is basically a substrate for many metabolic enzymes. It is used in different techniques like assays in which it is used to measure luciferase enzymes in the presence of adenosine triphosphate (ATP). Almost all the bioluminescence insects have specific types of luciferase enzymes but all they share a common substrate called D luciferin which is present in almost 40 different species of beetles or firefly insects(White et al., 1963, De Wet et al., 1985). It has a wide variety of applications, but more commonly it is a bioluminescent agent which is used in different in-vivo and in-vitro research related to stem cells, gene expression, growth of cancer, etc.

As we know, in general, reactive Oxygen species (ROS) are essential for the normal biological functions of cells but on other hand, they are also involved in cellular degeneration and tissue death. So, there is a basic need for such chemical or compound which is antioxidant in nature and very specific in its functions to combat the production of ROS (which induces cellular death) without altering the production of normal ROS that are required for cellular functions. That’s why Ferostatin-1 is developed. It is a very potent chemical agent for inhibiting ferroptosis which is defined as an oxidative, somewhat regulated, and nonapoptotic death of a cell.

n6-methyladenosine (m6A) is an internal RNA-modifying enzyme primarily catalyzed by the METTL3-METTL14 methyltransferase complex. The m6A methyltransferase METTL3 has been implicated in the initiation and maintenance of acute myeloid leukemia (AML), but the potential application of targeting this enzyme remains unclear. Here, we present the identification and characterization of STM2457 and the crystal structure of STM2457 in complex with METTL3-METTL14. STM2457 is a potent and selective METTL3 catalytic inhibitor, and tumor treatment with STM2457 resulted in decreased AML growth and increased differentiation and apoptosis. Concomitant with selective reduction of m6A levels of leukemic mRNAs, expression consistent with translational defects was reduced. The investigators demonstrated that METTL3 inhibition in vivo resulted in impaired engraftment and prolonged survival in various mouse models of AMTL, particularly targeting key stem cell subsets of AML. Collectively, these results reveal METTL3 inhibition as a potential therapeutic strategy against AML, and targeting of RNA-modifying enzymes is a promising anticancer therapeutic avenue.

When people are hungry, the stomach produces Ghrelin, which makes people feel hungry and promotes appetite, and after eating, fat cells produce Leptin, which makes people feel full and suppresses appetite. This homeostasis between ghrelin and leptin has long been thought to be critical for metabolism and diet.

The Human Genome Project (HGP) has successfully mapped the human genome, and human beings have also obtained their own "natural book". However, in the following 20 years, the interpretation of this "book from the sky" has been very difficult, and until now, we still lack sufficient understanding of the expression patterns of the human genome.

Trigeminal neuralgia is the most common cranial nerve disease, characterized by recurrent paroxysmal severe pain in the distribution area of the trigeminal nerve on one side of the face. The patient's daily activities, such as eating, drinking, talking, washing face, brushing teeth, etc., can cause severe pain, and some patients can even cause excruciating pain when the breeze blows. Therefore, trigeminal neuralgia is also known as "the first pain in the world", and some patients even choose to commit suicide to end the pain. Those who endure pain also tend to have lower quality of life, anxiety and depression.

Nature has long used large biomolecular assemblies to convert chemical energy into quasi-mechanical motion to perform essential functions of life. For example, cells use translation machinery -- the ribosome -- to make proteins from mRNA templates. Cellular nanomachines regulate the extent of translation by using miRNAs to guide AGO protein assembly into RISC to the 3' untranslated region. Similar to other machines, the ribonucleoprotein complex (RNP) requires the correct assembly of the correct number and type of molecules to perform its function. Any single misfolding or genetic mutation can lead to loss of function or abnormality and lead to disease. Intracellular single-molecule fluorescence microscopy (SMFM) imaging can be used to probe the structure and activity of individual machine components in their cellular environment. But the lack of high-yield methods to fluorescently label RNA molecules has hindered the development of SMFM.