【RNA Extraction】Upgraded products of traditional TRIZOL reagent, no need for Chloroform, room temperature, high extraction efficiency

In biological laboratory, RNA extraction is one of the most basal and commonly operation in molecular biology experiments. The total RNA extracted by TRIZOL reagent can be directly used in molecular biology experiments such as RT-PCR, qRT-PCR, Northern Blot, Dot Blot, in vitro translation, and high-throughput sequencing.

However, with the continuous upgrading of experimental requirements, although the traditional TRIzol (catalog number: GK20008) method is classic, it exposed problems gradually such as cumbersome operation, strong toxicity, and easy contamination. Especially due to the volatility and high toxicity of chloroform, which can damage the health of laboratory operators. People short-term inhaled of chloroform can cause symptoms such as dizziness, headache, nausea, fatigue, and drowsiness. If inhaled for a long time, it may cause damage to liver and kidney.

Now, a safer, more efficient, and purer RNA extraction reagent is available - the TRIzol NC Reagent produced by GLPbio (catalog number: GK20010) . This product has become the first choice for many laboratories to upgrade their RNA extraction operation.

Advantages of TRIzol NC Reagent product:

1. No need for chloroform or its substitutes, the operation becomes safer and more environment friendly

The TRIzol NC process requires no chloroform throughout the operation, that avoiding the harm caused by toxic and harmful gas. It is significantly reducing the health risks for operators.

2. The operation is more simple and friendly for beginners.

There is no three-phase separation during absorb RNA supernatant. When extracting the supernatant of the aqueous phase layer, there is no need to avoid touching the intermediate impurity layer additionally, which could greatly reduce the operation difficulty and improve the quality of RNA. The fault tolerance rate is high, and even beginners can successfully extract RNA at one attempt.

3. Full-process operation at room temperature, saving time and effort

TRIzol NC does not require low-temperature centrifugation or ice bath, that could reduce contamination contact. The entire process is completed at room temperature. Compared to the traditional TRIzol, it reduces the phase separation operation and can finish one extraction process in 1 hour approximately, making it more suitable for high-throughput method.

4. High RNA purity and few impurities, and more suitable for high-precision experiments followed

This product can effectively precipitate impurities such as proteins, polysaccharides, and DNA. The integrity of RNA products is high and the purity is also high. It is particularly suitable for analyses such as RT-qPCR, sequencing, and Northern Blot.

5. Wide application range

TRIzol NC could apply to many kinds of samples such as cultured cells, animal tissues, and plant tissues.

Comparison of RNA extraction using TRIzol NC Reagent and traditional TRIzol:

Fig1. Comparison of TRIzol NC operation process with conventional TRIzol operation process

Figure 2. Comparison of RNA extraction effects from 1×10⁷ HCT116 cells using traditional TRIzol Reagent (Catalog No.: GK20008) and TRIzol NC Reagent (Catalog No.: GK20010)