Solutions of High-performance Molecular Probes-1

1. Molecular probes for lipid droplets

Lipid droplets (LDs) are composed of a chemical substance called "lipids". Lipid droplets are storage organelles in the center of lipid and energy homeostasis. They have a unique structure composed of a hydrophobic core of neutral lipids, which is surrounded by a phospholipid monolayer modified by a specific set of proteins. Due to the diversity of lipid droplet functions, their abnormalities are associated with many pathological states.

Cat. No.	Product	Introduction
GC40165	C11 BODIPY 581/591	Ratio fluorescence indicator for lipid oxidation
GC42959	BODIPY 493/503	A green fluorescent probe widely used for lipid droplet labeling.
GC42960	BODIPY 505/515	A green fluorescent probe widely used for lipid droplet labeling.
GC42961	BODIPY 558/568 C12	Red neutral lipid droplet fluorescent probe
GC15539	Nile Red	Red neutral lipid droplet fluorescent probe
GC25676	Oil Red O	Commonly used tissue staining dyes can identify cholesterol esters.

Application Cases

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Figure 1. The lipid ROS levels of the target cells were detected using flow cytometry and C11 BODIPY 581/591.

Cells 11.22 (2022) 3580.

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Figure 2. Detection of lipid reactive oxygen species levels using BODIPY 581/591 C11 dye

Plos one 17.11 (2022)e0276083.

2. Molecular probes for cytomembrane

DiD, DiO, Dil and DiR are a group of lipophilic fluorescent dyes, mainly used for labeling cell membranes and other lipid-soluble biological structures. When these dyes bind to the cell membrane, their fluorescence intensity significantly increases. This is because they have high quenching constants and excited state lifetimes. Using appropriate concentrations, these dyes can uniformly stain the entire cell membrane. Each dye has its unique fluorescence color: Dil produces orange fluorescence, DiO produces green fluorescence, DiD produces red fluorescence, and DiR produces dark red fluorescence. This color difference makes them very suitable for multi-color imaging and flow cytometry analysis of living cells. The fluorescence of Dil and DiO can be observed through standard FITC and TRITC filters. The fluorescence of DiD can be excited by a 633 nm He-Ne laser, with its excitation and emission wavelengths being longer than those of Dil, making it more important in cell and tissue staining. The infrared fluorescence of DiR can penetrate cells and tissues, so it is often used for tracing in live imaging.

Cat. No.	Product	Ex/Em（nm）	Introduction
GC19515	DiO（DiOC18(3)）	484/510	Lipophilic fluorescent probe
GC33425	DiI (DiIC18(3))	550/567	Lipophilic fluorescent probe
GC30543	DiD perchlorate	644/663	Lipophilic fluorescent probe
GC35773	Cy7 DiC18（DiR ）	748/780	Near-infrared fluorescent dye

Application Cases

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Figure 3. Precisely identifying the DiR-labeled multipotent cells in the liver and spleen at different time points

World J Gastroenterol. 2012 Feb 14; 18(6): 507–516.

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Figure 4. Image of cells labeled with DID and DiO

3. Molecular probes for cell nucleus

Cat. No.	Product	Introduction
GC14228	Propidium Iodide	Fluorescent probes used for identifying dead cells.
GC43378	DAPI (hydrochloride)	Fluorescent DNA probe
GC10939	Hoechst 33342 trihydrochloride	Fluorescent DNA probe
GC12187	Hoechst 33258 trihydrochloride	Fluorescent DNA probe
GC36247	Hoechst 34580 tetrahydrochloride	Fluorescent DNA probe
GC33577	7-Aminoactinomycin D (7-AAD)	Fluorescent DNA probe
GC12913	Acridine Orange hydrochloride	Fluorescent nucleic acid dyes that can penetrate cells.
GC30066	NucPE1 (Nuclear Peroxy Emerald 1)	Nuclear localization fluorescent hydrogen peroxide probe.
GC36238	HOE 32021	An intracellular DNA dye.
GC26442	SYTO 9 Green Fluorescent Nucleic Acid Stain(5mM in DMSO)	It is a green fluorescent cell nucleus and chromosome counterstain.
GC30149	Pyronin Y (Pyronine G)	The targeting involves cellular structures including RNA, DNA and organelles.
GC64792	TO-PRO 1	DNA-binding fluorescent dyes are used as fluorescent dyes for necrotic cells.

Application Cases

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Figure 5. DAPI Detection Kit was used for TUNEL staining.

Life Sciences,Volume 271, 15 April 2021, 119199

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Figure 5. The Hoechst33342 was used to stain the nuclei.

Int J Mol Sci. 2023 May; 24(10): 8719.

4. Molecular probes for mitochondrion

Cat. No.	Product	Introduction
GC12331	JC-1	It is widely used for detecting mitochondrial membrane potential.
GC26229	Mito-Tracker Green	Mito-Tracker Green is a green fluorescent mitochondrial dye.
GC50454	MitoMark Red I	MitoMark Red I is a red fluorescent marker for mitochondria.
GC68230	MitoSOX Red	A novel fluorescent probe specifically targeting mitochondria in living cells.
GC15795	Rhodamine 123	Rhodamine dyes can bind to mitochondria and produce bright fluorescence.
GC30090	Rhodamine 6G	Rhodamine dyes can bind to mitochondria and produce bright fluorescence.
GC19476	Tetramethylrhodamine methyl ester (perchlorate)	Rhodamine dyes can bind to mitochondria and produce bright fluorescence.
GC30591	Rhodamine 800	Rhodamine dyes can bind to mitochondria and produce bright fluorescence.
GC38730	2-Di-1-ASP	Extensively used as fluorescent probes for mitochondrial staining and groove binding of double-stranded DNA.
GC30183	Acridine Orange 10-Nonyl Bromide (Nonylacridine orange)	Fluorescent probe for phosphatidylcholine (λex: 489 nm, λem: 525 nm).

Application Cases

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Figure 6. Mitochondrial morphology was detected by MitoTracker Red.

J. Agric. Food Chem. 2021, 69, 45, 13557-13567

5. Molecular probes for other cell structure

	Cat. No.	Product	Introduction
Endoplasmic reticulum	GB30171	ER-Tracker Blue-White DPX	A blue fluorescent dye with high selectivity for endoplasmic reticulum.
	GC64788	ER-Tracker Green	A green fluorescent dye with high selectivity for endoplasmic reticulum.
	GB30168	ER-Tracker Red	A red fluorescent dye with high selectivity for endoplasmic reticulum.
Golgi complex	GC19881	Golgi-Tracker Red	A red fluorescent dye with high selectivity for Golgi complex.
Golgi complex	GC43096	C6 NBD Ceramide (d18:1/6:0)	A green fluorescent dye with high selectivity for Golgi complex.
Lysosome	GC19882	Lyso-Tracker Red	A red fluorescent dye with high selectivity for lysosome.
	GC20101	Lyso-Tracker Green	A green fluorescent dye with high selectivity for lysosome.
	GC64677	Green DND-26	Green DND-26 is a green fluorescent dye for lysosomes.
Cellular skeleton	GC19997	FITC-Phalloidin	FITC-Phalloidin is a green fluorescent probe with high affinity and selectivity for filamentous actin (F-actin).
	GC18292	TRITC Phalloidin	TRITC-Phalloidin is a fluorescent probe for the orange-red form of F-actin.
	GC50523	Phalloidin-TFAX 488	Green fluorescent cytoskeletal staining,combined with actin.

Application Cases

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Figure 7. CLSM images show the co-localization of CRT and ER (ER-Tracker Green).

https://doi.org/10.1002/adfm.202214998

August 8, 2025 | Von: GLPBIO

Solutions of High-performance Molecular Probes-1

1. Molecular probes for lipid droplets

2. Molecular probes for cytomembrane

3. Molecular probes for cell nucleus

4. Molecular probes for mitochondrion

5. Molecular probes for other cell structure

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