SynaptoRedTM C2 (Synonyms: FM 4-64,FM1-43,SynaptoRedC2,Lipophilic probe,) |
| Katalog-Nr.GC14835 |
SynaptoRedTM C2, ein membranselektiver roter Fluoreszenzfarbstoff, gehÖrt zur FM-Familie der Styrylpyridinium-Farbstoffe.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 162112-35-8
Sample solution is provided at 25 µL, 10mM.
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SynaptoRedTM C2 is a membrane-selective red fluorescent dye and a highly lipophilic water-soluble styrene dye that can specifically bind to cell membranes and endomembranous organelles to produce fluorescence with an excitation wavelength of 530-560nm and an emission wavelength of 590-650nm[1, 2]. SynaptoRedTM C2 can be used as a marker for endocytic and exocytic membrane structures and is also used to track synaptic activity by staining synaptic vesicles at synapses or neuromuscular junctions[3]. SynaptoRedTM C2 can be used to stain bacteria (Gram-positive or Gram-negative) and yeast, but is rapidly internalized into the vacuole membrane in yeast[4, 5].
References:
[1] Betz W J, Mao F, Bewick G S. Activity-dependent fluorescent staining and destaining of living vertebrate motor nerve terminals[J]. Journal of Neuroscience, 1992, 12(2): 363-375.
[2] Kang S Y, Pokhrel A, Bratsch S, et al. Engineering Bacillus subtilis for the formation of a durable living biocomposite material[J]. Nature communications, 2021, 12(1): 7133.
[3] Fischer‐Parton S, Parton R M, Hickey P C, et al. Confocal microscopy of FM4‐64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae[J]. Journal of microscopy, 2000, 198(3): 246-259.
[4] Wang H Y, Hua X W, Jia H R, et al. Universal cell surface imaging for mammalian, fungal, and bacterial cells[J]. ACS Biomaterials Science & Engineering, 2016, 2(6): 987-997.
[5] Fischer‐Parton S, Parton R M, Hickey P C, et al. Confocal microscopy of FM4‐64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae[J]. Journal of microscopy, 2000, 198(3): 246-259.
This plan only provides a guide, please modify it to meet your specific needs.
1. Solution preparation
(1) Stock solution: Prepare SynaptoRedTM C2 stock solution with DMSO or EtOH at a concentration of 1-5mM.
Note: After unused stock solution is aliquoted, store it at -20°C or -80°C away from light to avoid repeated freezing and thawing.
(2) Working solution: Dilute the stock solution with a suitable buffer (such as serum-free culture medium, HBSS or PBS) to prepare SynaptoRedTM C2 working solution with a concentration of 5-20μM.
Note: The working solution must be prepared and used immediately. The final concentration of the working solution needs to be optimized according to different cell lines and experimental systems. It is recommended to start from the recommended concentration and explore the optimal concentration in a 10-fold range.
2. Suspended cell staining
(1) Suspended cells are centrifuged at 4°C and 1000rpm for 3-5min and the supernatant is discarded. Wash twice with PBS for 5min each time.
(2) Add 1mL of SynaptoRedTM C2 working solution and incubate at room temperature in the dark for 5-30min. The optimal culture time varies for different cells.
(3) After incubation, centrifuge at 1000-1500rpm for 5min, remove the supernatant, and wash 2-3 times with PBS, 5min each time.
(4) Resuspend the cells in pre-warmed serum-free cell culture medium or PBS. Observe by fluorescence microscopy or flow cytometry.
3. Staining of adherent cells
(1) Culture adherent cells on sterile coverslips.
(2) Remove the coverslip from the culture medium, aspirate the excess culture medium, and place the coverslip in a humid environment.
(3) Add 100μL SynaptoRedTM C2 working solution from one corner of the coverslip and gently shake to evenly cover all cells with the dye.
(4) Incubate at room temperature in the dark for 5-30min. The optimal culture time varies for different cells.
(5) Aspirate the dye working solution and wash the coverslip 2-3 times with culture medium. Observe by fluorescence microscopy.
Note: All fluorescent dyes have quenching problems. Please try to avoid light to slow down fluorescence quenching. For your safety and health, please wear a lab coat and disposable gloves when operating.
| Cas No. | 162112-35-8 | SDF | |
| Überlieferungen | FM 4-64,FM1-43,SynaptoRedC2,Lipophilic probe, | ||
| Chemical Name | 4-((1E,3E,5E)-6-(4-(diethylamino)phenyl)hexa-1,3,5-trien-1-yl)-1-(3-(triethylammonio)propyl)pyridin-1-ium bromide | ||
| Canonical SMILES | CC[N+](CC)(CC)CCC[N+]1=CC=C(/C=C/C=C/C=C/C2=CC=C(C=C2)N(CC)CC)C=C1.[Br-].[Br-] | ||
| Formula | C30H45Br2N3 | M.Wt | 607.51 |
| Löslichkeit | DMSO : 50 mg/mL (82.30 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.6461 mL | 8.2303 mL | 16.4606 mL |
| 5 mM | 329.2 μL | 1.6461 mL | 3.2921 mL |
| 10 mM | 164.6 μL | 823 μL | 1.6461 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 39 reference(s) in Google Scholar.)
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