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Cisplatin Verkauf (Synonyms: CDDP)

Katalog-Nr.GC11908

Cisplatin ist eines der besten und ersten metallbasierten Chemotherapeutika, das für eine breite Palette von soliden Krebsarten wie Hoden-, Eierstock-, Blasen-, Lungen-, Gebärmutterhals-, Kopf- und Halskrebs, Magenkrebs und einigen anderen Krebsarten verwendet wird.

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Cisplatin Chemische Struktur

Cas No.: 15663-27-1

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Sample solution is provided at 25 µL, 10mM.

Product has been cited by 5 publications

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

H69 SCLC cells

Reaction Conditions

H69 SCLC cells were treated with100 ng/ml cisplatin, to produce the H69-CP or 200 ng/ml cisplatin to obtain the H69CIS200 cells. These doses are below an IC50 for cisplatin and are within the range achieved in the clinical use of cisplatin.

Applications

The cells were 2- to 4-fold resistant to cisplatin, which could be used to further study the resistance mechanism.

Animal experiment [2]:

Animal models

Adult male Wistar rats, weighing 160-200 g

Preparation Method

The rats were kept at 25 °C on a 12/12 h light/dark cycle, in single plastic cages with bedding, with access to standard rat food and water ad libitum. Rats were randomly assigned to one of three groups: 1) Control group, who received no intervention and maintained a regular diet; 2) Gentamicin group, who were administered 100 mg/kg BW IP gentamicin daily for 7 days; 3) Cisplatin group, who were administered 1.5 mg/kg BW IP cisplatin twice a week for 3 weeks.

Dosage form

1.5 mg/kg

Applications

Cisplatin and gentamicin could significantly elevate serum levels of creatinine, uric acid, and urea, with cisplatin showing higher elevation. Cisplatin could also significantly decrease the GSH and GPx levels.

References:

[1]. Stordal B, et al. Understanding cisplatin resistance using cellular models. IUBMB Life. 2007 Nov;59(11):696-9.

[2]. Abouzed TK, et al. Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats. BMC Vet Res. 2021 Nov 16;17(1):350.

Background

Cisplatin ist eines der besten und ersten metallbasierten Chemotherapeutika, das für eine Vielzahl von soliden Krebsarten wie Hoden-, Eierstock-, Blasen-, Lungen-, Gebärmutterhals- und Kopf-Hals-Krebs, Magenkrebs und einige andere Krebsarten eingesetzt wird. Studien haben bestätigt, dass Cisplatin seine antikarzinogene Wirkung durch Angriffe an mehreren Stellen ausübt. Cisplatin bindet im Allgemeinen mit genomischer DNA (gDNA) oder mitochondrialer DNA (mtDNA), um DNA-Läsionen zu erzeugen, die Produktion von DNA, mRNA und Proteinen zu blockieren, die DNA-Replikation zu stoppen sowie mehrere Transduktionswege zu aktivieren, was schließlich zur Nekrose oder Apoptose führt.[1]

In vitro- und in vivo-Experimente zeigten, dass Cisplatin eine Zellresistenz induzierte und Ratten, die mit Cisplatin behandelt wurden, erhöhte Kreatinin-, Harnstoff- und Harnsäurewerte aufwiesen. Dieser Effekt war stärker ausgeprägt als bei Ratten, die mit Gentamicin behandelt wurden.[1][2]

References:
[1]. Stordal B, et al. Understanding cisplatin resistance using cellular models. IUBMB Life. 2007 Nov;59(11):696-9.
[2]. Abouzed TK, et al. Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats. BMC Vet Res. 2021 Nov 16;17(1):350.

Chemical Properties

Cas No. 15663-27-1 SDF
Überlieferungen CDDP
Chemical Name azane;dichloroplatinum(2+)
Canonical SMILES N.N.Cl[Pt+2]Cl
Formula Cl2H6N2Pt M.Wt 300.05
Löslichkeit 5 mg/mL in DMF (16.66 mM; DMSO can inactivate Cisplatin's activity), 1 mg/mL in Water (3.33 mM; DMSO can inactivate Cisplatin's activity) Storage 4°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 3.3328 mL 16.6639 mL 33.3278 mL
5 mM 0.6666 mL 3.3328 mL 6.6656 mL
10 mM 0.3333 mL 1.6664 mL 3.3328 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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