Cisplatin (Synonyms: CDDP) |
Katalog-Nr.GC11908 |
Cisplatin ist eines der besten und ersten metallbasierten Chemotherapeutika, das für eine breite Palette von soliden Krebsarten wie Hoden-, Eierstock-, Blasen-, Lungen-, Gebärmutterhals-, Kopf- und Halskrebs, Magenkrebs und einigen anderen Krebsarten verwendet wird.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 15663-27-1
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1]: | |
Cell lines |
H69 SCLC cells |
Reaction Conditions |
H69 SCLC cells were treated with100 ng/ml cisplatin, to produce the H69-CP or 200 ng/ml cisplatin to obtain the H69CIS200 cells. These doses are below an IC50 for cisplatin and are within the range achieved in the clinical use of cisplatin. |
Applications |
The cells were 2- to 4-fold resistant to cisplatin, which could be used to further study the resistance mechanism. |
Animal experiment [2]: | |
Animal models |
Adult male Wistar rats, weighing 160-200 g |
Preparation Method |
The rats were kept at 25 °C on a 12/12 h light/dark cycle, in single plastic cages with bedding, with access to standard rat food and water ad libitum. Rats were randomly assigned to one of three groups: 1) Control group, who received no intervention and maintained a regular diet; 2) Gentamicin group, who were administered 100 mg/kg BW IP gentamicin daily for 7 days; 3) Cisplatin group, who were administered 1.5 mg/kg BW IP cisplatin twice a week for 3 weeks. |
Dosage form |
1.5 mg/kg |
Applications |
Cisplatin and gentamicin could significantly elevate serum levels of creatinine, uric acid, and urea, with cisplatin showing higher elevation. Cisplatin could also significantly decrease the GSH and GPx levels. |
References: [1]. Stordal B, et al. Understanding cisplatin resistance using cellular models. IUBMB Life. 2007 Nov;59(11):696-9. [2]. Abouzed TK, et al. Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats. BMC Vet Res. 2021 Nov 16;17(1):350. |
Cisplatin ist eines der besten und ersten metallbasierten Chemotherapeutika, das für eine Vielzahl von soliden Krebsarten wie Hoden-, Eierstock-, Blasen-, Lungen-, Gebärmutterhals- und Kopf-Hals-Krebs, Magenkrebs und einige andere Krebsarten eingesetzt wird. Studien haben bestätigt, dass Cisplatin seine antikarzinogene Wirkung durch Angriffe an mehreren Stellen ausübt. Cisplatin bindet im Allgemeinen mit genomischer DNA (gDNA) oder mitochondrialer DNA (mtDNA), um DNA-Läsionen zu erzeugen, die Produktion von DNA, mRNA und Proteinen zu blockieren, die DNA-Replikation zu stoppen sowie mehrere Transduktionswege zu aktivieren, was schließlich zur Nekrose oder Apoptose führt.[1]
In vitro- und in vivo-Experimente zeigten, dass Cisplatin eine Zellresistenz induzierte und Ratten, die mit Cisplatin behandelt wurden, erhöhte Kreatinin-, Harnstoff- und Harnsäurewerte aufwiesen. Dieser Effekt war stärker ausgeprägt als bei Ratten, die mit Gentamicin behandelt wurden.[1][2]
References:
[1]. Stordal B, et al. Understanding cisplatin resistance using cellular models. IUBMB Life. 2007 Nov;59(11):696-9.
[2]. Abouzed TK, et al. Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats. BMC Vet Res. 2021 Nov 16;17(1):350.
Cas No. | 15663-27-1 | SDF | |
Überlieferungen | CDDP | ||
Chemical Name | azane;dichloroplatinum(2+) | ||
Canonical SMILES | N.N.Cl[Pt+2]Cl | ||
Formula | Cl2H6N2Pt | M.Wt | 300.05 |
Löslichkeit | 5 mg/mL in DMF (16.66 mM; DMSO can inactivate Cisplatin's activity), 1 mg/mL in Water (3.33 mM; DMSO can inactivate Cisplatin's activity) | Storage | 4°C, protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.3328 mL | 16.6639 mL | 33.3278 mL |
5 mM | 0.6666 mL | 3.3328 mL | 6.6656 mL |
10 mM | 0.3333 mL | 1.6664 mL | 3.3328 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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