Cyclo(RGDyK)

Name: Cyclo(RGDyK)
Brand: GlpBio
SKU: GC13923
Availability: InStock
Rating: 5 (22 reviews)

Katalog-Nr.GC13923

Cyclo(RGDyK) ist ein potenter und selektiver Inhibitor des αVβ3-Integrins mit einer IC50 von 20 nM.

Products are for research use only. Not for human use. We do not sell to patients.

Cyclo(RGDyK) Chemische Struktur

Cas No.: 250612-42-1

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10mM (in 1mL DMSO)	156,00 $	Auf Lager
5mg	102,00 $	Auf Lager
10mg	167,00 $	Auf Lager
25mg	334,00 $	Auf Lager
50mg	547,00 $	Auf Lager
100mg	825,00 $	Auf Lager

Tel：(909) 407-4943 Email: sales@glpbio.com

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Basiert auf Kundenrezensionen.

Sample solution is provided at 25 µL, 10mM.

Product has been cited by 2 publications

Description Protocol Chemical Properties Product Documents

Product Documents

Quality Control & SDS

View current batch:

Purity: >99.50%

Protocol

Cell experiment [1]:
Cell lines	B16-F10 cells and HUVEC
Preparation Method	Dil-loaded micellar formulations with different Cyclo(RGDyK) densities (0%, 10%, 20%) were incubated with B16-F10 cells or HUVEC in six-well plates for 3 h at 37°C at a final concentration of 0.5 ug/mL or 0.1 ug/mL Dil diluted in culture media, respectively. And the cells incubated with medium were used as negative controls. For the competition experiments, free Cyclo(RGDyK) (0.8 mM for B16-F10 and 1.6 µM for HUVEC) was pre-incubated with cells for 1 h, followed by continued co-incubation with Cyclo(RGDyK)-Dil-PM with 20% cRGDyK for 3 h. Then, cells were washed, trypsinized, and neutralized. After centrifugation at 1200 rpm for 5 min, cells were resuspended in PBS, followed by filtra
Reaction Conditions	0.8 mM for B16-F10 and 1.6 µM for HUVEC Cyclo(RGDyK) for 1 h
Applications	PEG-b-PLGA micelles without or with Cyclo(RGDyK) conjugation loaded with paclitaxel (PTX) or DiI were prepared and characterized. Drug-loaded micelles were stable in solution, with small diameters (
Animal experiment [2]:
Animal models	C57BL/6 mice
Preparation Method	Forty 3-month old female C57BL/6 mic were randomly allocated into four groups: (1) Sham-operated (Sham); (2) ovariectomized; (3) Ovx plus 8-week downhill running exercise (Ex); (4) Ovx plus exercise and received twice weekly injection of Cyclo(RGDyK) protein (a putative anti-irisin receptor agents) (ExRg).
Dosage form	2.5 mg/kg Cyclo(RGDyK) twice a week(tail vein injection)
Applications	Cyclo(RGDyK) administration weakened the exercise-related improvement of vBMD, BV/TV, and ALP intensity in bone.
References: [1]. Yin J, Li Z, et,al. Cyclic RGDyK conjugation facilitates intracellular drug delivery of polymeric micelles to integrin-overexpressing tumor cells and neovasculature. J Drug Target. 2011 Jan;19(1):25-36. doi: 10.3109/10611861003663531. Epub 2010 Mar 16. PMID: 20233083. [2]. Zhao R, Zhou Y, et,al. Irisin Regulating Skeletal Response to Endurance Exercise in Ovariectomized Mice by Promoting Akt/β-Catenin Pathway. Front Physiol. 2021 Mar 25;12:639066. doi: 10.3389/fphys.2021.639066. PMID: 33841178; PMCID: PMC8027323.

Cyclo(RGDyK) ist ein potenter und selektiver αVβ3-Integrin-Inhibitor mit einer IC50 von 20 nM^[1].

PEG-b-PLGA-Mizellen ohne oder mit Cyclo(RGDyK)-Konjugation, beladen mit Paclitaxel (PTX) oder DiI, wurden hergestellt und charakterisiert. Die medikamentenbeladenen Mizellen waren in Lösung stabil und hatten kleine Durchmesser (^[5]).

Ein neuartiges Medikamentenabgabesystem, das aus Cyclo(RGDyK)-modifizierten Fe3O4-Nanopartikeln mit hoher DOX-Last (R-DMP) besteht und magnetische Ausrichtung, Integrin alpha(v)beta3-Ausrichtung und hohe Arzneimittelladungseigenschaften kombiniert, wurde durch chemische Kopplung von Doxorubicin und Peptid Cyclo(RGDyK)) an den synthetischen Dualfunktionsmagnetnanopartikeln (DMP) unter Verwendung eines mehrfachen Kreuzverlinkers Poly-L-Glutaminsäure entwickelt. D-DMP zeigt eine verbesserte Aufnahme durch Tumorzellen, die Integrin alpha(v)beta3 exprimieren, und zeigt eine stärkere Zytotoxizität gegenüber Krebszellen^[2].

Die Verabreichung von Cyclo(RGDyK) schwächte die durch Bewegung verbesserte vBMD-, BV/TV- und ALP-Intensität im Knochen ab^[4]. Durch Blockieren des Irisin-Rezeptors (αV/β5) könnte Cyclo(RGDyK) irisininduzierte Signale reduzieren. Wenn die Irisin-Signalwege blockiert waren, wurden einige osteoblastogene Gene verringert, was zur durch Cyclo(RGDyK) verursachten Verringerung der osteogenen Differenzierung beitragen könnte^[3].

References:
[1]: Haubner R, Wester HJ, et,al. Glycosylated RGD-containing peptides: tracer for tumor targeting and angiogenesis imaging with improved biokinetics. J Nucl Med. 2001 Feb;42(2):326-36. PMID: 11216533.
[2]: Guo L, Ding W, et,al. The C(RgdyK)-conjugated Fe3O4 nanoparticles with high drug load for dual-targeting integrin alpha(v)beta3-expressing cancer cells. J Nanosci Nanotechnol. 2014 Jul;14(7):4858-64. doi: 10.1166/jnn.2014.8691. PMID: 24757954.
[3]: Kim H, Wrann CD, et,al. Irisin Mediates Effects on Bone and Fat via αV Integrin Receptors. Cell. 2018 Dec 13;175(7):1756-1768.e17. doi: 10.1016/j.cell.2018.10.025. Erratum in: Cell. 2019 Jul 11;178(2):507-508. PMID: 30550785; PMCID: PMC6298040.
[4]:Zhao R, Zhou Y, et,al. Irisin Regulating Skeletal Response to Endurance Exercise in Ovariectomized Mice by Promoting Akt/β-Catenin Pathway. Front Physiol. 2021 Mar 25;12:639066. doi: 10.3389/fphys.2021.639066. PMID: 33841178; PMCID: PMC8027323.
[5]:Yin J, Li Z, et,al. Cyclic RGDyK conjugation facilitates intracellular drug delivery of polymeric micelles to integrin-overexpressing tumor cells and neovasculature. J Drug Target. 2011 Jan;19(1):25-36. doi: 10.3109/10611861003663531. Epub 2010 Mar 16. PMID: 20233083.

Cas No.	250612-42-1	SDF
Chemical Name	2,2,2-trifluoroacetic acid compound with 2-((1Z,2S,3Z,5R,6Z,8S,9Z,11S,12Z)-8-(4-aminobutyl)-11-(3-guanidinopropyl)-3,6,9,12,15-pentahydroxy-5-(4-hydroxybenzyl)-1,4,7,10,13-pentaazacyclopentadeca-3,6,9,12,15-pentaen-2-yl)acetic acid (2:1)
Canonical SMILES	NCCCC[C@@]1([H])/C(O)=N/[C@@](/C(O)=N/C/C(O)=N/[C@@](/C(O)=N/[C@](/C(O)=N/1)([H])CC2=CC=C(O)C=C2)([H])CC(O)=O)([H])CCCNC(N)=N.FC(F)(F)C(O)=O.FC(F)(F)C(O)=O
Formula	C₃₁H₄₃F₆N₉O₁₂	M.Wt	847.72
Löslichkeit	DMSO : 100mg/mL; Water : 100mg/mL	Storage	Store at -20°C
General tips	Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition	Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
		1 mg	5 mg	10 mg
	1 mM	1.1796 mL	5.8982 mL	11.7963 mL
	5 mM	0.2359 mL	1.1796 mL	2.3593 mL
	10 mM	0.118 mL	0.5898 mL	1.1796 mL

Molaritätsrechner
Verdünnung-Rechner

Berechnen

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 22 reference(s) in Google Scholar.)

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Cyclo(RGDyK)

Kundenbewertungen

GlpBio Citations

Bioactive Compounds Premium Provider

Product has been cited by 2 publications

Product Documents

Quality Control & SDS

Protocol

Complete Stock Solution Preparation Table

Molaritätsrechner

Verdünnung-Rechner

In vivo Formulation Calculator (Clear solution)

Bewertungen

Cyclo(RGDyK)

Kundenbewertungen

GlpBio Citations

Bioactive Compounds Premium Provider

Product has been cited by 2 publications

Product Documents

Quality Control & SDS

Protocol

Complete Stock Solution Preparation Table

Molaritätsrechner

Verdünnung-Rechner

In vivo Formulation Calculator (Clear solution)

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