Deoxycholic acid (Cholanoic Acid) (Synonyms: Cholanoic Acid, DCA, NSC 8797) |
| Katalog-Nr.GC33762 |
Deoxycholic acid (Cholanoic Acid) is a bile acid and a byproduct of intestinal metabolism that can activate the G protein-coupled bile acid receptor TGR5.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 83-44-3
Sample solution is provided at 25 µL, 10mM.
Deoxycholic acid (Cholanoic Acid) is a bile acid and a byproduct of intestinal metabolism that can activate the G protein-coupled bile acid receptor TGR5[1]. Deoxycholic acid can induce tyrosine phosphorylation and activate the receptor tyrosine kinase epidermal growth factor receptor in a ligand-independent manner[2]. Deoxycholic acid is the first drug that can be used to reduce submental fat[3].
In vitro, Deoxycholic acid (0-1000µM) treatment of AR42J cells for 24h inhibited cell proliferation in a dose- and time-dependent manner, induced cell apoptosis and necrosis, and significantly increased the activities of transcription factor 2 (ATF2), interferon-stimulated response element (ISRE), NKX-2.5, androgen receptor (AR), p53, and hypoxia-inducible factor-1 (HIF-1), and reduced the activities of peroxisome proliferator-activated receptor (PPAR) and activator protein 1 (AP1)[4]. Treatment of colon cancer cells (SW480 and LoVo) with Deoxycholic acid (5, 50µM) for 30min significantly increased tyrosine phosphorylation of β-catenin, leading to the loss of E-cadherin binding to β-catenin in the cells[5]. Treatment of gastric cancer cell MGC803 cells with Deoxycholic acid (100µM) for 2h enhanced cell survival and proliferation activity under bile acid stress[6].
In vivo, Deoxycholic acid added to drinking water at a concentration of 0.2% for 12 weeks in Apcmin/+ mice increased the incidence of intestinal tumors, increased intestinal permeability and induced low-grade inflammation[7].
References:
[1] Truong J K, Dawson P A. Bile acid metabolism[J]. Biochemistry of Lipids, Lipoproteins and Membranes, 2021: 395-428.
[2] Jean-Louis S, Akare S, Ali M A, et al. Deoxycholic acid induces intracellular signaling through membrane perturbations[J]. Journal of Biological Chemistry, 2006, 281(21): 14948-14960.
[3] Farina G A, Cherubini K, de Figueiredo M A Z, et al. Deoxycholic acid in the submental fat reduction: A review of properties, adverse effects, and complications[J]. Journal of Cosmetic Dermatology, 2020, 19(10): 2497-2504.
[4] Zhang G, Zhang J, Shang D, et al. Deoxycholic acid inhibited proliferation and induced apoptosis and necrosis by regulating the activity of transcription factors in rat pancreatic acinar cell line AR42J[J]. In Vitro Cellular & Developmental Biology-Animal, 2015, 51: 851-856.
[5] Pai R, Tarnawski A S, Tran T. Deoxycholic acid activates β-catenin signaling pathway and increases colon cell cancer growth and invasiveness[J]. Molecular biology of the cell, 2004, 15(5): 2156-2163.
[6] Wang X, Sun L, Wang X, et al. Acidified bile acids enhance tumor progression and telomerase activity of gastric cancer in mice dependent on c‐Myc expression[J]. Cancer Medicine, 2017, 6(4): 788-797.
[7] Liu L, Dong W, Wang S, et al. Deoxycholic acid disrupts the intestinal mucosal barrier and promotes intestinal tumorigenesis[J]. Food & function, 2018, 9(11): 5588-5597.
| Cell experiment [1]: | |
Cell lines | AR42J cells |
Preparation Method | 2×104 cells in the logarithmic growth phase were seeded in each well of a 96-well plate. The medium was changed on the second day, and the fused cells were maintained for 24 h with medium containing different concentrations of Deoxycholic acid (0-1000µM). Then, 10μL MTT in PBSwith a concentration of 5g/L was added into each well for 4 h at 37°C. |
Reaction Conditions | 0-1000µM; 24h |
Applications | Deoxycholic acid inhibited AR42J cells proliferation in a dose- and time-dependent manner. |
| Animal experiment [2]: | |
Animal models | Apcmin/+ mice |
Preparation Method | Four-week-old female Apcmin/+ mice were obtained and randomly divided into 2 groups (n=10): a control group (receiving sterile water) and a Deoxycholic acid group (0.2% Deoxycholic acid in sterile water). After 12 weeks, the mice were sacrificed and intestinal tissues were obtained. |
Dosage form | Add 0.2% Deoxycholic acid to drinking water |
Applications | Deoxycholic acid increased the multiplicity of intestinal tumors and promoted tumorigenesis in mice. Deoxycholic acid increased intestinal permeability and induced low grade inflammation in mice. |
References: [1]Zhang G, Zhang J, Shang D, et al. Deoxycholic acid inhibited proliferation and induced apoptosis and necrosis by regulating the activity of transcription factors in rat pancreatic acinar cell line AR42J[J]. In Vitro Cellular & Developmental Biology-Animal, 2015, 51: 851-856. [2]Liu L, Dong W, Wang S, et al. Deoxycholic acid disrupts the intestinal mucosal barrier and promotes intestinal tumorigenesis[J]. Food & function, 2018, 9(11): 5588-5597. | |
| Cas No. | 83-44-3 | SDF | |
| Überlieferungen | Cholanoic Acid, DCA, NSC 8797 | ||
| Canonical SMILES | C[C@@]1([C@@]2([H])[C@H](C)CCC(O)=O)[C@](CC2)([H])[C@@](CC[C@@]3([H])[C@@]4(CC[C@@H](O)C3)C)([H])[C@]4([H])C[C@@H]1O | ||
| Formula | C24H40O4 | M.Wt | 392.57 |
| Löslichkeit | DMSO : ≥ 100 mg/mL (254.73 mM) | Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.5473 mL | 12.7366 mL | 25.4732 mL |
| 5 mM | 0.5095 mL | 2.5473 mL | 5.0946 mL |
| 10 mM | 0.2547 mL | 1.2737 mL | 2.5473 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 31 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *