Deoxycholic acid sodium salt (Sodium deoxycholate) |
| Katalog-Nr.GC33768 |
DesoxycholsÄure-Natriumsalz (Natriumdesoxycholat) (Natriumdesoxycholat), eine GallensÄure, ist ein Nebenprodukt des Darmstoffwechsels, das den G-Protein-gekoppelten GallensÄurerezeptor TGR5 aktiviert.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 302-95-4
Sample solution is provided at 25 µL, 10mM.
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Deoxycholic acid sodium salt (Sodium deoxycholate) is a bile acid that is a byproduct of intestinal metabolism and can activate the G protein-coupled bile acid receptor TGR5[1].
Deoxycholic acid sodium salt (Sodium deoxycholate) (100μM) induced the gastric cancer cell line MGC803 to produce cells resistant to acidified bile acids and enhanced its survival and proliferation activity under bile acid stress[1]. Deoxycholic acid sodium salt (Sodium deoxycholate) (50μM; 5min) inhibited PTPase activity in hepatocytes, thereby activating EGFR and MAPK pathways[2]. Deoxycholic acid sodium salt (Sodium deoxycholate) (50-150μM) significantly inhibited wound closure of cultured epithelial monolayer cells and attenuated cell migration in the Boyden chamber assay[3]. Deoxycholic acid sodium salt (Sodium deoxycholate) (0-300μM; 15h) reduced the gene expression of multiple pathways related to Caco-2 cell junctions (tight junctions, focal adhesions, gap junctions and adherens junction pathways) and increased permeability in a human intestinal barrier model [4]. Deoxycholic acid sodium salt (Sodium deoxycholate) (0-200μM; 24h) dose-dependently induced NLRP3 inflammasome activation and production of the highly proinflammatory cytokine IL-1β in macrophages [5].
Enema of Deoxycholic acid sodium salt (Sodium deoxycholate) (4mM in 1ml PBS; 7 days) resulted in a significant decrease in body weight and shortened colon length in mice, and a significant increase in fecal blood scores and MPO activity [5]. Deoxycholic acid sodium salt (Sodium deoxycholate) (20-300mg/kg; 10h) increased the nuclear damage induced by 1,2-dimethylhydrazine (DMH) in colonocytes in C57BL/6J mice [6].
References:
[1].Wang X, Sun L, Wang X, et al. Acidified bile acids enhance tumor progression and telomerase activity of gastric cancer in mice dependent on c‐Myc expression[J]. Cancer Medicine, 2017, 6(4): 788-797.
[2].Qiao L, Studer E, Leach K, et al. Deoxycholic acid (DCA) causes ligand-independent activation of epidermal growth factor receptor (EGFR) and FAS receptor in primary hepatocytes: inhibition of EGFR/mitogen-activated protein kinase-signaling module enhances DCA-induced apoptosis[J]. Molecular biology of the cell, 2001, 12(9): 2629-2645.
[3]. Mroz M S, Lajczak N K, Goggins B J, et al. The bile acids, deoxycholic acid and ursodeoxycholic acid, regulate colonic epithelial wound healing[J]. American Journal of Physiology-Gastrointestinal and Liver Physiology, 2018, 314(3): G378-G387.
[4]. Zeng H, Safratowich B D, Cheng W H, et al. Deoxycholic acid modulates cell-junction gene expression and increases intestinal barrier dysfunction[J]. Molecules, 2022, 27(3): 723.
[5]. Zhao S, Gong Z, Zhou J, et al. Deoxycholic acid triggers NLRP3 inflammasome activation and aggravates DSS-induced colitis in mice[J]. Frontiers in Immunology, 2016, 7: 536.
[6]. Suzuki K, Bruce W R. Increase by deoxycholic acid of the colonic nuclear damage induced by known carcinogens in C57BL/6J mice[J]. Journal of the National Cancer Institute, 1986, 76(6): 1129-1132.
| Cell experiment [1]: | |
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Cell lines |
SW480 and LoV2 cell |
|
Preparation Method |
Serum-starved cells were incubated with Deoxycholic acid sodium salt (Sodium deoxycholate) (5-100μM) for 30-120min. |
|
Reaction Conditions |
5-100μM; 30-120min |
|
Applications |
Low concentrations of Deoxycholic acid sodium salt (Sodium deoxycholate) (5-50μM) significantly increase tyrosine phosphorylation of β-catenin, induce urokinase-type plasminogen activator, uPAR, and cyclin D1 expression and enhance colon cancer cell proliferation and invasiveness. |
| Animal experiment [2]: | |
|
Animal models |
Acute colitis modle |
|
Preparation Method |
Acute colitis was induced in C57BL/6 mice using 2.5% DSS dissolved in drinking water with free access to water for 7 consecutive days. Animals in the DSS-treated group were randomly divided into two groups and received PBS, 4mM Deoxycholic acid sodium salt (Sodium deoxycholate) (in PBS, 0.1ml) enema for 7 consecutive days starting from the first day of DSS treatment. |
|
Dosage form |
4mM in 0.1ml PBS; 7 days; enema. |
|
Applications |
Addition of 4 mM Deoxycholic acid sodium salt (Sodium deoxycholate) enema resulted in a significant decrease in body weight and shortened colon length, and a significant increase in fecal blood scores and MPO activity in mice. |
|
References: |
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| Cas No. | 302-95-4 | SDF | |
| Canonical SMILES | C[C@@]1([C@@]2([H])[C@H](C)CCC([O-])=O)[C@](CC2)([H])[C@@](CC[C@@]3([H])[C@@]4(CC[C@@H](O)C3)C)([H])[C@]4([H])C[C@@H]1O.[Na+] | ||
| Formula | C24H39NaO4 | M.Wt | 414.55 |
| Löslichkeit | DMSO : 6 mg/mL (14.47 mM) | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4123 mL | 12.0613 mL | 24.1225 mL |
| 5 mM | 0.4825 mL | 2.4123 mL | 4.8245 mL |
| 10 mM | 0.2412 mL | 1.2061 mL | 2.4123 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 40 reference(s) in Google Scholar.)
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