Meisoindigo (Synonyms: Methylisoindigotin) |
| Katalog-Nr.GC14312 |
Meisoindigo (Dian III), ein Derivat von Indirubin, stoppt den Zellzyklus in der G0/G1-Phase und induziert die Apoptose in Zellen der primÄren akuten myeloischen LeukÄmie (AML). Meisoindigo weist eine hohe AntitumoraktivitÄt auf.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 97207-47-1
Sample solution is provided at 25 µL, 10mM.
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Meisoindigo is a potential agent for acute myeloid leukemia [1].
Meisoindigo is a synthetic modification of indirubin. It has been used for chronic myeloid leukemia in China with less toxicity. In the in vitro assay, it can inhibit synthesis of DNA and RNA and the assembly of microtubules. It is also reported to have efficacy in acute myeloid leukemia. In the AML cell lines, HL-60, NB4 and U937, meisoindigo induces apoptosis in both caspase-dependent and -independent pathways. The effect induced by meisoindigo is likely mediated through the intrinsic mitochondrial pathway. Meisoindigo also induces cell cycle arrest with more cells in sub-G1 and G0/G1 phases and fewer cells in the S phase. Besides, meisoindigo is found to induce differentiation in HL-60 and NB4 cell lines. The expression of hTERT can be down-regulated by meisoindigo, which can enhance the anti-leukemic activity of chemotherapeutic agents. Moreover, meisoindigo shows a moderate anti-tumor efficacy in NOD/SCID mice injected with AML cells [1].
References:
[1] Lee CC, Lin CP, Lee YL, Wang GC, Cheng YC, Liu HE. Meisoindigo is a promising agent with in vitro and in vivo activity against human acute myeloid leukemia. Leuk Lymphoma. 2010 May;51(5):897-905.
| Cas No. | 97207-47-1 | SDF | |
| Überlieferungen | Methylisoindigotin | ||
| Chemical Name | (3E)-1-methyl-3-(2-oxo-1H-indol-3-ylidene)indol-2-one | ||
| Canonical SMILES | CN1C2=CC=CC=C2C(=C3C4=CC=CC=C4NC3=O)C1=O | ||
| Formula | C17H12N2O2 | M.Wt | 276.29 |
| Löslichkeit | DMF: 16 mg/ml,DMF:PBS (pH 7.2) (1:4): 0.20 mg/ml,DMSO: 1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.6194 mL | 18.0969 mL | 36.1939 mL |
| 5 mM | 723.9 μL | 3.6194 mL | 7.2388 mL |
| 10 mM | 361.9 μL | 1.8097 mL | 3.6194 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 39 reference(s) in Google Scholar.)
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