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(S)-3'-hydroxy Blebbistatin (Synonyms: (-)-3'-hydroxy Blebbistatin, meta-hydroxy-Blebbistatin, m-hydroxy-Blebbistatin)

Katalog-Nr.GC41484

(S)-3'-hydroxy Blebbistatin is a more stable and less phototoxic form of (-)-blebbistatin, which is a selective cell-permeable inhibitor of non-muscle myosin II ATPases.

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(S)-3'-hydroxy Blebbistatin Chemische Struktur

Cas No.: 2097136-42-8

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

(S)-3'-hydroxy Blebbistatin is a more stable and less phototoxic form of (-)-blebbistatin , which is a selective cell-permeable inhibitor of non-muscle myosin II ATPases. (-)-Blebbistatin rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC50s = 0.5-5 µM), while poorly inhibiting smooth muscle myosin (IC50 = 80 µM). Through these effects, it blocks apoptosis-related bleb formation, directed cell migration, and cytokinesis in vertebrate cells. However, prolonged exposure to blue light (450-490 nm) results in degradation of blebbistatin to an inactive product via cytotoxic intermediates, which may be problematic for its use in fluorescent live cell imaging applications. The addition of a 3'-hydroxy group decreases the inherent fluorescence while retaining the activity of (-)-blebbistatin. (S)-3'-hydroxy-Blebbistatin has the same stereochemistry as the active (-)-blebbistatin enantiomer.

Chemical Properties

Cas No. 2097136-42-8 SDF
Überlieferungen (-)-3'-hydroxy Blebbistatin, meta-hydroxy-Blebbistatin, m-hydroxy-Blebbistatin
Canonical SMILES O=C1[C@@]2(O)C(N(C3=CC=CC(O)=C3)CC2)=NC4=C1C=C(C)C=C4
Formula C18H16N2O3 M.Wt 308.3
Löslichkeit DMF: 20 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 12.5 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 3.2436 mL 16.218 mL 32.4359 mL
5 mM 0.6487 mL 3.2436 mL 6.4872 mL
10 mM 0.3244 mL 1.6218 mL 3.2436 mL
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**Bei der Herstellung von Stammlösungen ist immer das chargenspezifische Molekulargewicht von

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