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Scrambled 10Panx (Synonyms: Scrambled 10Panx Phe-Ser-Val-Tyr-Trp-Ala-Gln-Ala-Asp-Arg)

Katalog-Nr.GC11058

Panx-1 mimetic inhibitory peptide, blocks pannexin-1 gap junctions

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Scrambled 10Panx Chemische Struktur

Cas No.: 1315378-72-3

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1mg
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

View current batch:

Protocol

Cell experiment [1]:

Cell lines

Cultured human subcutaneous fibroblasts.

Preparation method

Soluble to 0.50 mg/ml in sterile water. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reaction Conditions

100 μM.

Applications

In cultured human subcutaneous fibroblasts, 10panx decreases the bradykinin-induced [Ca2+]i response and attenuates bradykinin (30 μM)-induced ATP release [1]. In oocytes, 10panx inhibits pannexin1 currents. In oocytes expressing pannexin1, 10panx attenuates dye uptake by pannexin1 channels [2].

References:

[1]. Pinheiro AR, Paramos-de-Carvalho D, Certal M, et al. Bradykinin-induced Ca2+ signaling in human subcutaneous fibroblasts involves ATP release via hemichannels leading to P2Y12 receptors activation. Cell Commun Signal, 2013, 11: 70.

[2]. Wang J, Ma M, Locovei S, et al. Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Am J Physiol Cell Physiol. 2007, 293(3): C1112-1119.

Background

Scrambled 10Panx is the scrambled form of 10Panx (WRQAAFVDSY), a mimetic peptide of pannexin 1 that inhibits dye uptake by macrophages without affecting cellular membrane currents. Being structurally similar to anther peptide PanxE1B, 10Panx is three amino acid shorter but exerting an inhibitory effect against pannexin1 currents in oocytes to a similar extent as PanxE1b. 10Panx is also able to block the activation of I2nd, decrease the bradykinin-induced [Ca2+]i response and attenuate bradykinin-induced ATP release from human fibroblasts loaded with quinacrine. However, in several studies, scrambled 10Panx has been proved to be less effective than 10Panx in its authentic form.

Reference

[1].Roger J. Thompson, Michael F. Jackson, Michelle E. Olah, Ravi L. Rungta, Dustin J. Hines, Michael A. Beazely, John F. MacDonald, Brian A. MacVicar. Activation of Pannexin-1 hemichannels augments aberrant bursting in the hippocampus. Science 2008; 322: 1555-1559.
[2].Ana Rita Pinheiro, Diogo Paramos-de-Carvalho, Marianna Certal, Cristina Costa, Maria Teresa Magalhaes-Cardoso, Fatima Ferreirinha, Maria Adelina Costa and Paulo Correia-de-Sa. Bradykinin-induced Ca2+ signaling in human subcutaneous fibroblasts involves ATP release via hemichannels leading to P2Y12 receptors activation. Communication and Signaling 2013, 11:70
[3].Junjie Wang, Meiyun Ma, Silviu Locovei, Robert W. Keane and Gerhard Dahl. Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Am J Physiol Cell Physiol 293: C1112-C1119, 2007

Chemical Properties

Cas No. 1315378-72-3 SDF
Überlieferungen Scrambled 10Panx Phe-Ser-Val-Tyr-Trp-Ala-Gln-Ala-Asp-Arg
Canonical SMILES CC(C)C(C(=O)NC(CC1=CC=C(C=C1)O)C(=O)NC(CC2=CNC3=CC=CC=C32)C(=O)NC(C)C(=O)NC(CCC(=O)N)C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC(CCCN=C(N)N)C(=O)O)NC(=O)C(CO)NC(=O)C(CC4=CC=CC=C4)N
Formula C58H79N15O16 M.Wt 1242.37
Löslichkeit ≥ 31.05mg/mL in DMSO Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 0.8049 mL 4.0246 mL 8.0491 mL
5 mM 0.161 mL 0.8049 mL 1.6098 mL
10 mM 0.0805 mL 0.4025 mL 0.8049 mL
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