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Dihydrorhodamine 123 (DHR 123)

Catalog No.GC30581

Dihydrorhodamine 123 (DHR 123) Chemical Structure

A cellpermeable fluorogenic probe used as an indicator of intracellular peroxynitrite formation

Size Price Stock Qty
1 mg
$45.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & SDS

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Protocol

Cell experiment:

The HL60 cells are incubated at 6×106 cells/mL in Krebs-Ringer buffer at 37°C containing 10 μM Dihydrorhodamine 123 (DHR). The generation of O2- is initiated by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) and the progress of the generation of rhodamine 123 is monitored in 50-μL aliquots (3×105 cells) diluted tenfold before analysis. The uninduced HL60 cells are loaded with 5 μM carboxy SNARF-1 AM acetate (SNARF-AM) in the Na+ medium for 10 min at 37°C and washed by centrifugation and resuspension to remove unhydrolysed SNARF ester[1].

References:

[1]. Lydia M. Henderson et al. Dihydrorhodamine 123: a fluorescent probe for superoxide generation Eur.J.Biochem. 217, 973-980.

Background

Dihydrorhodamine 123 is a fluorescent probe (λex=488 nm, λem=525 nm).

In the presence of 10 μM Dihydrorhodamine 123 (DHR) the stimulation of the neutrophil NADPH oxidase by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) resultes in an increase in the rate of rhodamine generation. The fluorescent intensity of the cells, in the presence of 10 μM Dihydrorhodamine 123, increases with time following the addition of 50 nM PMA. In the presence of 10 μM Dihydrorhodamine 123, induced HL60 cells show a sustained increase in fluorescence following the addition of 50 nM PMA[1].

[1]. Lydia M. Henderson et al. Dihydrorhodamine 123: a fluorescent probe for superoxide generation? Eur.J.Biochem. 217, 973-980.

Chemical Properties

Cas No. 109244-58-8 SDF
Synonyms N/A
Chemical Name N/A
Canonical SMILES O=C(OC)C1=CC=CC=C1C2C3=C(OC4=C2C=CC(N)=C4)C=C(N)C=C3
Formula C21H18N2O3 M.Wt 346.38
Solubility DMSO: 100 mg/mL (288.70 mM) Storage Store at -20°C,unstable in solution, ready to use.
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Average Rating: 5 ★★★★★ (Based on Reviews and 38 reference(s) in Google Scholar.)

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