Dihydrotestosterone (Synonyms: stanolone,5α-DHT) |
| Catalog No.GC19064 |
Dihydrotestosterone(DHT) is an endogenous androgenic steroid and hormone that acts as an agonist for androgen receptors.
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Cas No.: 521-18-6
Sample solution is provided at 25 µL, 10mM.
Dihydrotestosterone(DHT) is an endogenous androgenic steroid and hormone that acts as an agonist for androgen receptors.
Dihydrotestosterone regulation of miRNA expression. CD44 expression and cell adhesion to hyaluronic acid (HA) were down-regulated when cells were treated with Dihydrotestosterone or transfection with a miR-328-3p mimic[1]. Altered miRNA levels when luminal breast cancer MCF-7 cancer cancer cells were treated with Dihydrotestosterone [2]. The expression of one critical miRNA, namely let-7a-d, was also found to be up-regulated in Dihydrotestosterone-treated MDA-MB-453 cells [3]. Treatment of MDA-MB-453 cells with Dihydrotestosterone causes down-regulation of miR-125b and miR-100 in association of increased expression of matrix metalloprotease 13, a target of both miRNAs [4]. Dihydrotestosterone treatment enhanced STAT5 phosphorylation and promoted proliferation of all CRPC cells. On immunofluorescence, activation of STAT5 and GR translocating into the nucleus after Dihydrotestosterone treatment [5].
Dihydrotestosterone induced early hair regression, hair miniaturization, hair density loss, and changes in hair morphology in male C57BL/6 mice [6]. The presence of the possible negative regulation of cell proliferation by Dihydrotestosterone. Moreover, cell proliferation related to urethral tube formation was revealed to be Dihydrotestosterone dose dependent [7]. Dihydrotestosterone-treated ovariectomized mice had free access to food (free-feeding), they had increased food intake and higher body weight compared with control animals. These mice also had a significantly greater accumulation of fat in the liver and exhibited increased fasting glucose, impaired glucose tolerance, and resistance to leptin [8]. Promastigotes in the presence of Dihydrotestosterone produced significantly larger lesions in BALB/c earlobes [9].
References:
[1]. Al-Othman N, Hammad H, et,al.Dihydrotestosterone regulates expression of CD44 via miR-328-3p in triple-negative breast cancer cells. Gene. 2018 Oct 30;675:128-135. doi: 10.1016/j.gene.2018.06.094. Epub 2018 Jun 28. PMID: 29964098.
[2]. Nakano K, Miki Y, et,al. Identification of androgen-responsive microRNAs and androgen-related genes in breast cancer. Anticancer Res. 2013 Nov;33(11):4811-9. PMID: 24222117.
[3]. Lyu S, Yu Q, et,al. Androgen receptor decreases CMYC and KRAS expression by upregulating let-7a expression in ER-, PR-, AR+ breast cancer. Int J Oncol. 2014 Jan;44(1):229-37. doi: 10.3892/ijo.2013.2151. Epub 2013 Oct 25. PMID: 24172884.
[4]. Ahram M, Mustafa E, et,al.Differential expression and androgen regulation of microRNAs and metalloprotease 13 in breast cancer cells. Cell Biol Int. 2017 Dec;41(12):1345-1355. doi: 10.1002/cbin.10841. Epub 2017 Sep 5. PMID: 28816390.
[5]. Song C, Kim Y, et,al. Dihydrotestosterone enhances castration-resistant prostate cancer cell proliferation through STAT5 activation via glucocorticoid receptor pathway. Prostate. 2014 Sep;74(12):1240-8. doi: 10.1002/pros.22841. Epub 2014 Jul 7. PMID: 25043756.
[6]. Fu D, Huang J, et,al. Dihydrotestosterone-induced hair regrowth inhibition by activating androgen receptor in C57BL6 mice simulates androgenetic alopecia. Biomed Pharmacother. 2021 May;137:111247. doi: 10.1016/j.biopha.2021.111247. Epub 2021 Jan 29. PMID: 33517191.
[7]. Suzuki H, Matsushita S, et,al. 5α-Dihydrotestosterone negatively regulates cell proliferation of the periurethral ventral mesenchyme during urethral tube formation in the murine male genital tubercle. Andrology. 2017 Jan;5(1):146-152. doi: 10.1111/andr.12241. Epub 2016 Oct 1. PMID: 27696776.
[8]. Kanaya N, Vonderfecht S, et,al. Androgen (dihydrotestosterone)-mediated regulation of food intake and obesity in female mice. J Steroid Biochem Mol Biol. 2013 Nov;138:100-6. doi: 10.1016/j.jsbmb.2013.04.001. Epub 2013 May 7. PMID: 23665441; PMCID: PMC4130703.
[9]. SÁnchez-GarcÍa L, Wilkins-Rodriguez A, et,al. Dihydrotestosterone enhances growth and infectivity of Leishmania Mexicana. Parasite Immunol. 2018 Mar;40(3). doi: 10.1111/pim.12512. Epub 2018 Jan 18. PMID: 29272044.
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