E3 ligase Ligand 2 (Synonyms: 4-hydroxy Thalidomide) |
Catalog No.GC32848 |
E3 ligase Ligand 2 (Cereblon ligand 2) is the Thalidomide-based Cereblon ligand used in the recruitment of CRBN protein. E3 ligase Ligand 2 (Cereblon ligand 2) can be connected to the ligand for protein by a linker to form PROTACs.
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Cas No.: 5054-59-1
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
E3 ligase Ligand 2 is a Ligand for E3 Ligase used in PROTAC technology.
Thalidomide may exert anti-angiogenic effect in isolated blood vessels and endothelial cells. E3 ligase Ligand 2 is a putative hydroxylated thalidomide metabolite. E3 ligase Ligand 2 has weak anti-angiogenic activity (14% mean inhibition of blood vessel density at 100 mg). E3 ligase Ligand 2 does not have any anti-proliferative effect against the breast or neuroblastoma cells, but do possess appreciable anti-proliferative activity against the endothelial cells[1].
[1]. Marks MG, et al. Effects of putative hydroxylated thalidomide metabolites on blood vessel density in the chorioallantoic membrane (CAM) assay and on tumor and endothelial cell proliferation. Biol Pharm Bull. 2002 May;25(5):597-604.
Cas No. | 5054-59-1 | SDF | |
Synonyms | 4-hydroxy Thalidomide | ||
Canonical SMILES | OC1=CC=CC(C(N2C3C(NC(CC3)=O)=O)=O)=C1C2=O | ||
Formula | C13H10N2O5 | M.Wt | 274.23 |
Solubility | DMSO : ≥ 36.5 mg/mL (133.10 mM) | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.6466 mL | 18.2329 mL | 36.4657 mL |
5 mM | 0.7293 mL | 3.6466 mL | 7.2931 mL |
10 mM | 0.3647 mL | 1.8233 mL | 3.6466 mL |
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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