Filipin Complex (Synonyms: U-5956) |
| Catalog No.GC18406 |
Filipin complex is a neutral polyene,it has antifungal activity.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 11078-21-0
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Filipin complex is a neutral polyene,it has antifungal activity.As a fluorescent compound, Filipin complex can be used for sterol imaging in labeled biological structures,Excitation/emission values of the complexes were 338/480 nm[1，4] . Filipin III (HY-N6718) is the main component of Filipin complex which is composed of four parts[3] . The interaction of Filipin Complex with steroids was found to be related both to the functional group at the 3-position and the aliphatic chain of the steroid[7] .
Cells were pretreated with 1 and 5 g/mL of Filipin Complex for 1 h and infected with IPNV at a MOI of 1 for 1 h. After 12 h of incubation in the presence of the drug, IPNV VP2/VP3 expression was revealed by immunofluorescence. It shows the effect of Filipin Complex on IPNV infection. No effect on viral infection was observed[2] . The Filipin Complex complex can bind various sterols in aqueous solutions and fungal cell membranes, especially 24α-methyl cholesterol, 24α-ethyl cholesterol, and cholesterol, inducing membrane pit formation and leakage of cell contents[5] . The Filipin Complex complex can inhibit cell growth and mitochondrial terminal electron transport in Saccharomyces cerevisiae[6] .
References:
[1]. Castanho MA, Coutinho A, et,al.Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol. J Biol Chem. 1992 Jan 5;267(1):204-9. PMID: 1730589
[2]. Levican J, Miranda-CÁrdenas C, et,al. Infectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis. Sci Rep. 2017 Jun 8;7(1):3068. doi: 10.1038/s41598-017-03036-w. PMID: 28596575; PMCID: PMC5465193.
[3]. Payero TD, Vicente CM, et,al. Functional analysis of filipin tailoring genes from Streptomyces filipinensis reveals alternative routes in filipin III biosynthesis and yields bioactive derivatives. Microb Cell Fact. 2015 Aug 7;14:114. doi: 10.1186/s12934-015-0307-4. PMID: 26246267; PMCID: PMC4527110.
[4]. KÜhnl A, Musiol A,et,al.Late Endosomal/Lysosomal Cholesterol Accumulation Is a Host Cell-Protective Mechanism Inhibiting Endosomal Escape of Influenza A Virus. mBio. 2018 Jul 24;9(4):e01345-18. doi: 10.1128/mBio.01345-18. PMID: 30042202; PMCID: PMC6058292.
[5]. Kitajima Y, Sekiya T,et,al. Freeze-fracture ultrastructural alterations induced by filipin, pimaricin, nystatin and amphotericin B in the plasmia membranes of Epidermophyton, Saccharomyces and red complex-induced membrane lesions. Biochim Biophys Acta. 1976 Dec 2;455(2):452-65. doi: 10.1016/0005-2736(76)90317-5. PMID: 793632.
[6]. SHAW PD, ALLAM AM,et,al. EFFECT OF FILIPIN ON THE TERMINAL ELECTRON TRANSPORT SYSTEM OF SACCHAROMYCES CEREVISIAE. Biochim Biophys Acta. 1964 Jul 8;89:33-41. doi: 10.1016/0926-6569(64)90098-7. PMID: 14213010.
[7]. Kleinschmidt MG, Chough KS. Effect of filipin on liposomes prepared with different types of steroids. Plant Physiol. 1972 May;49(5):852-6. doi: 10.1104/pp.49.5.852. PMID: 16658060; PMCID: PMC366064.
Average Rating: 5 (Based on Reviews and 5 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *