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Filipin Complex Catalog No.GC18406

A 28-membered ring pentaene macrolide antifungal antibiotic

Size Price Stock Qty
10mM * 1 mL in DMSO
$123.00
In stock
5mg
$85.00
In stock
10mg
$135.00
In stock
50mg
$378.00
In stock

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Sample solution is provided at 25 µL, 10mM.

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Chemical Properties

Cas No. 11078-21-0 SDF Download SDF
Synonyms N/A
Chemical Name N/A
Canonical SMILES N/A
Formula C35H58O11 (for Filipin III) M.Wt 654.8
Solubility DMF: Soluble,DMSO: Soluble,Ethanol: Soluble,Methanol: Soluble Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
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Background

Filipin complex is a neutral polyene originally isolated from S. filipinensis with antifungal activity. It inhibits cell growth as well as mitochondrial terminal electron transport in S. cerevisiae when used at a concentration of 135 ug/ml. Filipin complex binds to various sterols, particularly 24α-methyl cholesterol 24α-ethyl cholesterol, and cholesterol, in aqueous solutions and in fungal cell membranes when used at a concentration of 50 ug/ml, inducing membrane pit formation and leakage of cell contents. It is a fluorescent compound that has been used to label sterols within biological structures for imaging. Filipin complex displays excitation/emission maxima of 338/480 nm, respectively.

References:
[1]. Shaw, P.D., Allam, A.M., and Gottlieb, D. Effect of filipin on the terminal electron transport system of Saccharomyces cerevisiae Biochim. Biophys. Acta 89(1), 33-41 (1964).
[2]. Kitajima, Y., Sekiya, T., and Nozawa, Y. Freeze-fracture ultrastructural alterations induced by filipin, pimaricin, nystatin and amphotericin B in the plasmia membranes of Epidermophyton, Saccharomyces and red complex-induced membrane lesions Biochim Biophys. Acta. 455(2), 452-465 (1976).
[3]. Miller, R.G. Mini Review. The use and abuse of filipin to localize cholesterol in membranes Cell Biol. Int. Rep. 8(7), 519-535 (1984).
[4]. Kleinschmidt, M.G., and Chough, K.S. Effect of filipin on liposomes prepared with different types of steroids Plant Physiol. 49(5), 852-856 (1972).
[5]. Castanho, M.A., Coutinho, A., and Prieto, M.J. Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol J. Biol. Chem. 267(1), 204-209 (1992).
[6]. Sekiya, T., Takenawa, T., and Nozawa, H. Reorganization of membrane cholesterol during membrane fusion in myogenesis in vitro: A study using the filipin-cholesterol complex Cell Struct. Funct. 9(2), 143-155 (1984).
[7]. Bridgman, P.C., and Nakajima, Y. Distribution of filipin-sterol complexes on cultured muscle cells: Cell-substratum contact areas associated with acetylcholine receptor clusters J. Cell Biol. 96(2), 363-372 (1983).