|Filipin Complex Catalog No.GC18406|
Sample solution is provided at 25 µL, 10mM.
|Cas No.||11078-21-0||SDF||Download SDF|
|Formula||C35H58O11 (for Filipin III)||M.Wt||654.8|
|Solubility||Soluble in DMSO||Storage||Store at -20°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
Filipin complex is a neutral polyene originally isolated from S. filipinensis with antifungal activity. It inhibits cell growth as well as mitochondrial terminal electron transport in S. cerevisiae when used at a concentration of 135 ug/ml. Filipin complex binds to various sterols, particularly 24α-methyl cholesterol 24α-ethyl cholesterol, and cholesterol, in aqueous solutions and in fungal cell membranes when used at a concentration of 50 ug/ml, inducing membrane pit formation and leakage of cell contents. It is a fluorescent compound that has been used to label sterols within biological structures for imaging. Filipin complex displays excitation/emission maxima of 338/480 nm, respectively.
. Shaw, P.D., Allam, A.M., and Gottlieb, D. Effect of filipin on the terminal electron transport system of Saccharomyces cerevisiae Biochim. Biophys. Acta 89(1), 33-41 (1964).
. Kitajima, Y., Sekiya, T., and Nozawa, Y. Freeze-fracture ultrastructural alterations induced by filipin, pimaricin, nystatin and amphotericin B in the plasmia membranes of Epidermophyton, Saccharomyces and red complex-induced membrane lesions Biochim Biophys. Acta. 455(2), 452-465 (1976).
. Miller, R.G. Mini Review. The use and abuse of filipin to localize cholesterol in membranes Cell Biol. Int. Rep. 8(7), 519-535 (1984).
. Kleinschmidt, M.G., and Chough, K.S. Effect of filipin on liposomes prepared with different types of steroids Plant Physiol. 49(5), 852-856 (1972).
. Castanho, M.A., Coutinho, A., and Prieto, M.J. Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol J. Biol. Chem. 267(1), 204-209 (1992).
. Sekiya, T., Takenawa, T., and Nozawa, H. Reorganization of membrane cholesterol during membrane fusion in myogenesis in vitro: A study using the filipin-cholesterol complex Cell Struct. Funct. 9(2), 143-155 (1984).
. Bridgman, P.C., and Nakajima, Y. Distribution of filipin-sterol complexes on cultured muscle cells: Cell-substratum contact areas associated with acetylcholine receptor clusters J. Cell Biol. 96(2), 363-372 (1983).