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FKBP52 Monoclonal Antibody (Clone Hi52C)

Catalog No.GC49695

For immunochemical analysis of FKBP52

Products are for research use only. Not for human use. We do not sell to patients.

FKBP52 Monoclonal Antibody (Clone Hi52C) Chemical Structure

Cas No.: N/A

Size Price Stock Qty
25 µg
$341.00
In stock
100 µg
$558.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more co-chaperones.1 One class of Hsp90-binding co-chaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TRP co-chaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins.2 FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex.3 The N-terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPlase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TRP repeats involved in protein-protein interactions with Hsp40.4 Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by the glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics.3,5 Also, whereas FKBP51 typically has a role with the progesterone receptor, FKBP52 has been found to be linked to the progesterone, androgen, and glucocorticoid receptors.5

1.Cheung-Flynn, J., Roberts, P.J., Riggs, D.L., et al.C-terminal sequences outside the tetratricopeptide repeat domain of FKBP51 and FKBP52 cause differential binding to Hsp90The Journal of Biological Chemisty278(19)17388-17394(2003) 2.Davies, T.H., Ning, Y.M., and SÁnchez, E.R.A new first step in activation of steroid receptors. Hormone-induced switching of FKBP51 and FKBP52 immunophilinsThe Journal of Biological Chemisty277(7)4597-4600(2002) 3.Wu, B., Li, P., Liu, Y., et al.3D structure of human FK506-binding protein 52: Implications for the assembly of the glucocorticoid receptor/Hsp90/immunophilin hetrocomplexThe Journal of Biological Chemisty101(22)8348-8353(2004) 4.Denny, W.B., Prapapanich, V., Smith, D.F., et al.Structure-function analysis of squirrel monkey FK506-binding protein 51, a potent inhibitor of glucocorticoid receptor activityEndocrinology146(7)3194-3201(2005) 5.Cox, M.B., Riggs, D.L., Hessling, M., et al.FK506-binding protein 52 phosphorylation: A potential mechanism for regulating steroid hormone receptor activityMol. Endocrinol.21(12)2956-2967(2007)

Chemical Properties

Cas No. N/A SDF Download SDF
Canonical SMILES N/A
Formula N/A M.Wt 52
Solubility N/A Storage -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 19.2308 mL 96.1538 mL 192.3077 mL
5 mM 3.8462 mL 19.2308 mL 38.4615 mL
10 mM 1.9231 mL 9.6154 mL 19.2308 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Reviews

Review for FKBP52 Monoclonal Antibody (Clone Hi52C)

Average Rating: 5 ★★★★★ (Based on Reviews and 25 reference(s) in Google Scholar.)

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