Flavopiridol (Synonyms: Alvocidib, HL 275, HMR 1275, L-868,275)

Cell lines

23 human tumor cells

Preparation method

This compound is soluble in DMSO. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

0.1 ng/mL ~ 10 μg/mL; 6 ~ 18 days

Applications

In 3 prostate cancer cell lines (PRXFI369, PC3MX and LNCaPX) and 1 melanoma cell line (MEXFI341), Flavopiridol potently inhibited colony formation, even at the concentration down to 0.1 ng/mL. At the concentrations between 10 and 100 ng/mL, Flavopiridol significantly inhibited colony formation of human bone marrow cells. For most of the human tumor cells, the inhibitory effect of Flavopiridol on colony formation was first observed at 10 ng/mL.

Animal models

Nude mice bearing PRXFI369 xenografts

Dosage form

10 mg/kg/day; p.o.

Applications

In tumor-bearing nude mice, Flavopiridol caused 12.5% deaths at the maximum tolerated dose (MTD). In nude mice bearing PRXFI369 xenografts, Flavopiridol at MTD resulted in an optimal T/C value of 33% and a growth delay of 30 days. However, at day 28, tumor volume increased slightly to 115%. Flavopiridol at the dose of 15 mg/kg/day was toxic, whereas at the dose of 5 mg/kg/day was inactive.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Drees M, Dengler WA, Roth T, Labonte H, Mayo J, Malspeis L, Grever M, Sausville EA, Fiebig HH. Flavopiridol (L86-8275): selective antitumor activity in vitro and activity in vivo for prostate carcinoma cells. Clin Cancer Res. 1997 Feb;3(2):273-9.