Fluo-3AM (Synonyms: Fluo-3 Acetoxymethyl ester) |
| Catalog No.GC33437 |
Fluo-3AM(Fluo-3-pentaacetoxymethyl ester) is a calcium indicator that exhibit an increase in fluorescence upon binding Ca2+; used to image the spatial dynamics of Ca2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening.
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Cas No.: 121714-22-5
Sample solution is provided at 25 µL, 10mM.
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Fluo-3AM is one of the most commonly used fluorescent probes to detect intracellular calcium ion concentration.After it penetrates the cell membrane and enters the cell, it is cleaved by the esterase in the cell to form Fluo-3, so as to be retained in the cell.Fluo-3 is almost non-fluorescent when it exists in the form of free ligand, but it can produce strong fluorescence when it combines with intracellular calcium ions, with a maximum excitation wavelength of 506nm and a maximum emission wavelength of 526nm. In actual detection, the recommended excitation wavelength is about 488nm and the emission wavelength is 525-530nm. Changes in intracellular calcium concentration can be detected using laser confocal microscopy or flow cytometry[1-4].
References:
[1]. Loughrey CM, MacEachern KE, et,al. Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics. Cell Calcium. 2003 Jul;34(1):1-9. doi: 10.1016/s0143-4160(03)00012-5. PMID: 12767887.
[2]. Ji S, Li S, Zhao X, et,al.Protective role of phenylethanoid glycosides, Torenoside B and Savatiside A, in Alzheimer's disease. Exp Ther Med. 2019 May;17(5):3755-3767. doi: 10.3892/etm.2019.7355. Epub 2019 Mar 7. PMID: 30988761; PMCID: PMC6447766.
[3]. Williams PDE, Verma S, et,al.Adapting techniques for calcium imaging in muscles of adult Brugia malayi. Invert Neurosci. 2020 Aug 16;20(3):12. doi: 10.1007/s10158-020-00247-1. PMID: 32803437; PMCID: PMC7891862.
[4]. Williams PDE, Kashyap SS, et,al.Diethylcarbamazine, TRP channels and Ca2+ signaling in cells of the Ascaris intestine. Sci Rep. 2022 Dec 9;12(1):21317. doi: 10.1038/s41598-022-25648-7. PMID: 36494409; PMCID: PMC9734116.
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