Forsythoside B |
| Catalog No.GN10727 |
Forsythoside B is a phenylethanol glycoside isolated from the leaves of the traditional Chinese medicinal plant Forsythia suspensa. It has multiple biological activities and can be used to treat inflammatory diseases and promote blood circulation
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 81525-13-5
Sample solution is provided at 25 µL, 10mM.
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Forsythoside B is a phenylethanol glycoside isolated from the leaves of the traditional Chinese medicinal plant Forsythia suspensa. It has multiple biological activities and can be used to treat inflammatory diseases and promote blood circulation[1]. Forsythoside B can target pneumolysin to reduce the virulence of Streptococcus pneumoniae[2]. Forsythoside B exhibits effective antibacterial activity against Pseudomonas aeruginosa and Acinetobacter baumannii[3].
In vitro, pretreatment of BV-2 cells with Forsythoside B (1, 2.5µM) for 3h significantly inhibited the LPS-induced increase in intracellular IL-6, TNF-α, iNOs, NO and IL-1β[4]. Pretreatment of RAW 264.7 macrophages with Forsythoside B (40, 80, 160μg/mL) for 1h significantly inhibited the LPS-induced increase in intracellular PGE2, COX-2, nitrite and iNOS[5].
In vivo, Forsythoside B (40mg/kg) was intravenously injected into rats with cecal ligation and puncture (CLP)-induced sepsis, which reduced serum TNF-α, IL-6, HMGB1, triggering receptor expressed on myeloid cells (TREM-1), and endotoxin levels, upregulated IL-10 levels, and reduced myeloperoxidase (MPO) activity in the lungs, liver, and small intestine[6]. Forsythoside B (10, 40mg/kg) was intraperitoneally injected into mice with spinal cord injury (SCI), which significantly reduced the levels of inflammatory factors, inhibited glial scar formation, reduced neuronal death and tissue defects, and promoted motor recovery[7].
References:
[1] Bailly C. Forsythosides as essential components of Forsythia-based traditional Chinese medicines used to treat inflammatory diseases and COVID-19[J]. World Journal of Traditional Chinese Medicine, 2022, 8(1): 1-20.
[2] Wang Z, Sun Y, Gu K, et al. Forsythoside B, the active component of Frosythiae fructuse water extract, alleviates Streptococcus pneumoniae virulence by targeting pneumolysin[J]. Journal of Applied Microbiology, 2024, 135(10): lxae251.
[3] Shi Z, Zhang J, Wang Y, et al. Antibacterial effect and mechanisms of action of forsythoside B, alone and in combination with antibiotics, against Acinetobacter baumannii and Pseudomonas aeruginosa[J]. Phytomedicine, 2024, 135: 156038.
[4] Kong F, Jiang X, Wang R, et al. Forsythoside B attenuates memory impairment and neuroinflammation via inhibition on NF-κB signaling in Alzheimer’s disease[J]. Journal of Neuroinflammation, 2020, 17: 1-15.
[5] Liu J, Li X, Yan F, et al. Protective effect of forsythoside B against lipopolysaccharide-induced acute lung injury by attenuating the TLR4/NF-κB pathway[J]. International Immunopharmacology, 2019, 66: 336-346.
[6] Jiang W L, Zhang S P, Zhu H B. Forsythoside B protects against experimental sepsis by modulating inflammatory factors[J]. Phytotherapy Research, 2012, 26(7): 981-987.
[7] Xia M, Zhang Y, Wu H, et al. Forsythoside B attenuates neuro-inflammation and neuronal apoptosis by inhibition of NF-κB and p38-MAPK signaling pathways through activating Nrf2 post spinal cord injury[J]. International Immunopharmacology, 2022, 111: 109120.
| Cell experiment [1]: | |
Cell lines | BV-2 cells |
Preparation Method | BV-2 cells seeded into 96-well plates were pretreated with 1μM and 2.5μM Forsythoside B or PBS for 3h. Then co-incubated with 1μg/mL LPS dissolved in DMEM for 24h. |
Reaction Conditions | 1, 2.5µM; 3h |
Applications | The LPS-induced enhancements of IL-6, TNF-α, iNOs, NO, and IL-1β in BV-2 cells were strongly suppressed by Forsythoside B pre-incubation. |
| Animal experiment [2]: | |
Animal models | Sprague-Dawley rats |
Preparation Method | Forty caecal ligation and puncture (CLP) rats were divided into four subgroups, with 10 rats in each group: (i) CLP (model group); (ii) Forsythoside B 40mg/kg group; (iii) Imipenem 20mg/kg group; (iv) Imipenem 20mg/kg+Forsythoside B 40mg/kg group. All rats received only two doses of Forsythiaside B, which were intravenously injected (i.v.) at 1h and 13h after CLP, and the small intestine, liver, and lung were collected for biochemical analysis at 20h after CLP. |
Dosage form | 40mg/kg; i.v. |
Forsythoside B can reduce serum TNF-α, IL-6, HMGB1, triggering receptor expressed on myeloid cells (TREM-1) and endotoxin levels, while serum IL-10 levels are upregulated and lung, liver and small intestine myeloperoxidase (MPO) levels are decreased. | |
References: | |
| Cas No. | 81525-13-5 | SDF | |
| Chemical Name | [(2R,3R,4R,5R,6R)-2-[[(2S,3S)-3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]oxymethyl]-6-[2-(3,4-dihydroxyphenyl)ethoxy]-5-hydroxy-4-[(2R,3S,4S,5S,6R)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-3-yl] (E)-3-(3,4-dihydroxyphenyl)prop-2-enoate | ||
| Canonical SMILES | CC1C(C(C(C(O1)OC2C(C(OC(C2OC(=O)C=CC3=CC(=C(C=C3)O)O)COC4C(C(CO4)(CO)O)O)OCCC5=CC(=C(C=C5)O)O)O)O)O)O | ||
| Formula | C34H44O19 | M.Wt | 756.7 |
| Solubility | ≥ 35.6mg/mL in EtOH with ultrasonic; 100 mg/mL in DMSO; 100 mg/mL in Water | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.3215 mL | 6.6076 mL | 13.2153 mL |
| 5 mM | 0.2643 mL | 1.3215 mL | 2.6431 mL |
| 10 mM | 0.1322 mL | 0.6608 mL | 1.3215 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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