Accueil>>Signaling Pathways>> Neuroscience>>(±)-10-hydroxy-12(Z)-Octadecenoic Acid-d5

(±)-10-hydroxy-12(Z)-Octadecenoic Acid-d5 (Synonyms: 10-hydroxy-cis-12-Octadecenoic Acid-d5)

Catalog No.GC52421

An internal standard for the quantification of (±)-10-hydroxy-12(Z)-octadecenoic acid

Products are for research use only. Not for human use. We do not sell to patients.

(±)-10-hydroxy-12(Z)-Octadecenoic Acid-d5 Chemical Structure

Taille Prix Stock Qté
25 μg
216,00 $US
En stock
50 μg
410,00 $US
En stock
100 μg
734,00 $US
En stock

Tel:(909) 407-4943 Email: sales@glpbio.com

Avis des clients

Based on customer reviews.

  • GlpBio Citations

    GlpBio Citations
  • Bioactive Compounds Premium Provider

    Bioactive Compounds Premium Provider

Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

View current batch:

Background

(±)-10-hydroxy-12(Z)-Octadecenoic acid-d5 is intended for use as an internal standard for the quantification of (±)-10-hydroxy-12(Z)-octadecenoic acid by GC- or LC-MS. (±)-10-hydroxy-12(Z)-Octadecenoic acid is an oxylipin and a metabolite of linoleic acid .1 It is formed from linoleic acid by conjugated linoleic acid-hydrase (CLA-HY) as an intermediate in CLA biosynthesis and can also be produced from linoleic acid by gut microbiota. (±)-10-hydroxy-12(Z)-Octadecenoic acid (30 µM) inhibits LPS-induced nitric oxide (NO) production, ERK phosphorylation, and increases in inducible NO synthase (iNOS) levels in BV-2 microglia cells.2 It reduces TNF-α, NO2, and IL-10 levels in LPS-stimulated and -unstimulated isolated dendritic bone marrow cells when used at a concentration of 100 µM.3 (±)-10-hydroxy-12(Z)-Octadecenoic acid (100 µM) decreases LPS-induced maturation of dendritic cells in isolated mouse bone marrow cells.

1.Kishino, S., Takeuchi, M., Park, S.-B., et al.Polyunsaturated fatty acid saturation by gut lactic acid bacteria affecting host lipid compositionProc. Natl. Acad. Sci. USA110(44)17808-17813(2013) 2.Ikeguchi, S., Izumi, Y., Kitamura, N., et al.Inhibitory effect of the gut microbial linoleic acid metabolites, 10-oxo-trans-11-octadecenoic acid and 10-hydroxy-cis-12-octadecenoic acid, on BV-2 microglial cell activationJ. Pharmacol. Sci.138(1)9-15(2018) 3.Bergamo, P., Luongo, D., Miyamoto, J., et al.Immunomodulatory activity of a gut microbial metabolite of dietary linoleic acid, 10-hydroxy-cis-12-octadecenoic acid, associated with improved antioxidant/detoxifying defencesJ. Funct. Foods11192-202(2014)

Chemical Properties

Cas No. SDF Download SDF
Synonymes 10-hydroxy-cis-12-Octadecenoic Acid-d5
Canonical SMILES OC(C/C=C\CCCC([2H])([2H])C([2H])([2H])[2H])CCCCCCCCC(O)=O
Formula C18H29D5O3 M.Wt 303.5
Solubility Ethanol: 1 mg/mL Storage -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 3.2949 mL 16.4745 mL 32.9489 mL
5 mM 0.659 mL 3.2949 mL 6.5898 mL
10 mM 0.3295 mL 1.6474 mL 3.2949 mL
  • Molarity Calculator

  • Dilution Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

Calculate

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Avis

Review for (±)-10-hydroxy-12(Z)-Octadecenoic Acid-d5

Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%
Review for (±)-10-hydroxy-12(Z)-Octadecenoic Acid-d5

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.